Culture Collections

Antimicrobial Susceptibility Controls

 

Antimicrobial Susceptibility PlatePublic Health England's National Collection of Type Cultures (NCTC) offers a wide range of strains that can be used to control antimicrobial susceptibility testing to:

  • monitor test performance and quality of the materials used
  • confirm that the in-use method will detect resistance

NCTC strains are verified by Public Health England's Antimicrobial Resistance and Healthcare Associated Infections Reference Unit (AMRHAI) and are used in diagnostic testing laboratories worldwide.

The strains listed are specifically recommended by one or more of the following:

  • the European Committee on Antimicrobial Susceptibility Testing (EUCAST)
  • Clinical and Laboratory Standards Institute (CLSI)
  • United Kingdom Standards for Microbiology Investigations (formerly National Standard Methods)
  • British Society of Antimicrobial Chemotherapy (BSAC)

 

Download the Antimicrobial Susceptibility Testing Control Strains leaflet. You may also be interested in Antimicrobial Resistance Reference Strains

 

 

European Committee on Antimicrobial Susceptibility Testing (EUCAST)

Recommended Strains for Routine and Extended Internal Quality Control (Version 7.0)

Organism

NCTC® Strain Reference

Routine or extended internal QC

Characteristics

Other collection number

Campylobacter jejuni

 NCTC 11351

Routine internal QC

 - 

ATCC 33560; CCUG 11284; CIP 702; DSM 4688

Enterococcus faecalis

 NCTC 12697

Routine internal QC

 -  

ATCC 29212; CECT 795; CIP 103214; CCUG 9997; DSM 2570; WDCM 00087

Enterococcus faecalis

 

 NCTC 13379

Extended internal QC

High-level gentamicin resistant (HLGR)

ATCC 51299; CCUG 34289; CIP 104676; DSM 12956; WDCM 00085; WDCM 00152

Escherichia coli

 

 NCTC 12241

Routine internal QC

 -  

ATCC 25922; CECT 434; CIP 76.24; CCUG 17620; DSM 1103; NCIMB 12210; WDCM 00013

Escherichia coli

 

 NCTC 11954

Routine internal QC 

TEM-1 β-lactamase (Non-ESBL) producer 

ATCC 35218; CECT 943; CIP 102181; CCUG 30600; DSM 5923

Escherichia coli

 

 NCTC 13846

Routine internal QC

MCR-1 positive

 - 

Haemophilus influenzae

 NCTC 12975

Routine internal QC

 -  

ATCC 49766; CCUG 29539; CIP 103570; DSM 11970

Haemophilus influenzae

 

 NCTC 12699

Extended internal QC

 -  

ATCC 49247; CCUG 26214; CIP 104604; DSM 9999

Klebsiella pneumoniae 

 

 NCTC 13368

Routine and extended internal QC 

SHV-18 ESBL producing 

ATCC 700603; CECT 7787; CCUG 45421

Pseudomonas aeruginosa

 

 NCTC 12903

Routine internal QC

 - 

CCUG 67181; WDCM 00212

ATCC 27853; CECT 108; CIP 76.110; CCUG 17619; DSM 1117; WDCM 00025

Staphylococcus aureus

 

 NCTC 12493

Routine internal QC 

Methicillin resistant (MRSA), mecA positive 

CCUG 67181; WDCM 00212

Staphylococcus aureus

 

 NCTC 12973

Routine internal QC 

Weak β-lactamase positive, mecA negative

ATCC 29213; CECT 794; CIP 103429; CCUG 15915; DSM 2569; JCM 2874; WDCM 00131

Streptococcus pneumoniae

 NCTC 12977

Routine internal QC

Penicillin intermediate (altered penicillin binding protein)

ATCC 49619; CCUG 33638; CIP 104340; DSM11967


 

Clinical and Laboratory Standards Institute (CLSI)

Recommended performance standards for antimicrobial susceptibility testing (M100-S24)

ReferenceCharacteristOrganism

NCTC® Strain   Reference

Characteristics

Other collection number

Bacteroides fragilis

NCTC 9343

β-lactamase positive

ATCC 25285; CN 6300;

DSM 2151

Bacteroides thetaiotaomicron

NCTC 13706

β-lactamase positive

 -

Enterococcus faecalis

NCTC 12697

 -

ATCC 29212; WDCM 00087

Enterococcus faecalis

NCTC 13379

Resistant to vancomycin (VanB) and high-level aminoglycosides

ATCC 51299; CIP 10467; WDCM 00085; WDCM   00152

Enterococcus faecalis

NCTC 13705

 -

WDCM 00210

Escherichia coli

NCTC 12241

β-lactamase negative

ATCC 25922; DSM 1103; NCIMB 12210; WDCM   00013

Escherichia coli

NCTC 11954

Contains plasmid-encoded TEM-1 β-lactamase (Non-ESBL) producer

ATCC 35218

Haemophilus influenzae

NCTC 12699

BLNAR (β-lactamase negative, ampicillin resistant)

ATCC 49247

Haemophilus influenzae

NCTC 12975

Ampicillin susceptible

ATCC 49766; CIP 103570

Haemophilus influenzae

NCTC 13377

 -

ATCC 10211; CIP 103708

Helicobacter pylori

NCTC 11637

 -

ATCC 43504; CCUG 17874; DSM 4867; LMG 7539;

Klebsiella pneumoniae

NCTC 13368

SHV-18 ESBL producing

ATCC 700603; CCUG 45421; LMG 20218

Neisseria gonorrhoeae

NCTC 12700

CMRNG (Chromosome-mediated resistant Neisseria gonorrhoeae)

ATCC 49226

Pseudomonas aeruginosa

NCTC 12903

Contains inducible AmpC β-lactamase

ATCC 27853; WDCM 00025

Staphylococcus aureus

NCTC 12981

β-lactamase negative, mecA negative,   little value in MIC testing due to its extreme susceptibility to most drugs

ATCC 25923; CIP 76.25; DSM 1104; JCM 2413; WDCM 00034

Staphylococcus aureus

NCTC 12973

Weak β-lactamase positive, mecA negative

ATCC 29213; CIP 103429; DSM 2569; JCM   2874; WDCM 00131

Staphylococcus aureus

NCTC 13373

Oxacillin resistant, mecA negative

ATCC 43300; WDCM 00211

Streptococcus pneumoniae

NCTC 12977

Penicillin intermediate (altered penicillin binding protein)

ATCC 49619; CIP 104340

 

UK Standard for Microbiology Investigations (UKSMI)

Recommended in B59: Enterobacteriaceae producing Extended Spectrum b-lactamases

Organism

NCTC® Strain Reference

Characteristics

Other collection number

Escherichia coli

NCTC 13353

CTX-M-15 (cefotaxime)

 -

Escherichia coli

NCTC 13351

TEM-3 (Broad Spectrum)

 -

Escherichia coli

NCTC 13352

TEM-10 (ceftazidimase)

 -

Escherichia coli

NCTC 10418

Negative control

ATCC 10536; CN 6455; DSM 682; NCIB 8879;

Klebsiella pneumoniae

NCTC 13368

SHV-18 ESBL producing

ATCC 700603; CCUG 45421; LMG 20218

 

 

Recommended in B60: Detection of bacteria with carbapenem-hydrolysing b-lactamases (carbapenemases)

Organism

NCTC® Strain Reference

Characteristics

Other collection number

Class A Carbapenemase

Klebsiella pneumoniae

NCTC 13438  

Member of the international ST258 clone producing KPC-3 carbapenemase

-

Class B Carbapenemases (Metallo-β-lactamases)

Escherichia coli

NCTC 13476  

IMP-type (unsequenced)

CCUG 68729

Klebsiella pneumoniae

NCTC 13439  

VIM-1; QnrS1 (outbreak   strain)

-

Klebsiella pneumoniae

NCTC 13440  

VIM-1; QnrS1 (sporadic)

-

Klebsiella pneumoniae

NCTC 13443  

New Delhi Metallo-β-lactamase   (NDM-1)

CCUG 68728

Pseudomonas aeruginosa

NCTC 13437  

VIM-10; VEB-1

-

Class D Carbapenemases (OXA   carbapenemases)

Acinetobacter baumannii

NCTC 13301  

OXA-23 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13302  

OXA-25 (OXA-24/40-like) (also   with OXA-51-like)

-

Acinetobacter baumannii

NCTC 13303  

OXA-26 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13304  

OXA-27 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13305  

(A 15) OXA-58 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13421  

OXA-23 Clone 2 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13424  

OXA-23 Clone 1 (also with   OXA-51-like)

-

Acinetobacter baumannii

NCTC 13420  

SE Clone OXA-51-like

-

Acinetobacter baumannii

NCTC 13422  

NW Clone OXA-51-like

-

Acinetobacter baumannii

NCTC 13423  

T strain (UK3) OXA-51-like

-

Klebsiella pneumoniae

NCTC 13442  

Sequence type 353 with OXA-48  

CCUG 68727

Note: Either E. coli NCTC 10418 or ATCC 25922 should be used as a negative control in confirmation tests

 

World Health Organisation (WHO)

Neisseria gonorrhoeae panel

Organism

NCTC® Strain Reference

WHO Designation

Characteristics

Other collection number

Neisseria   gonorrhoeae

NCTC 13477

WHO F

Fully susceptible isolate

CCUG 57595

Neisseria   gonorrhoeae

NCTC 13478

WHO G

Resistant to; ciprofloxacin (Low-level), penicillin (intermediate) tetracycline (TRNG).

CCUG 57596

Neisseria   gonorrhoeae

NCTC 13479

WHO K

Resistant to; cefixime (low-level),   ciprofloxacin (high-level), penicillin G, tetracycline.

CCUG 57597

Neisseria   gonorrhoeae

NCTC 13480

WHO L

Resistant to; azithromycin (intermediate), penicillin G, ceftriaxone (low-level), ciprofloxacin (high-level), tetracycline

CCUG 57598

Neisseria   gonorrhoeae

NCTC 13481

WHO M

Resistant to; ciprofloxacin, penicillin G (penicillinase-producing), tetracycline

CCUG 57599

Neisseria   gonorrhoeae

NCTC 13482

WHO N

Resistant to; penicillin G (penicillinase-producing), ciprofloxacin, tetracycline (TRNG)

CCUG 57600

Neisseria   gonorrhoeae

NCTC 13483

WHO O

Resistant to; penicillin G (penicillinase-producing), spectinomycin, tetracycline

CCUG 57601

Neisseria   gonorrhoeae

NCTC 13484

WHO P

Resistant to; azithromycin, penicillin G (intermediate), tetracycline (intermediate).

CCUG 57602

Neisseria   gonorrhoeae

NCTC 13817

WHO U

Resistant to; azithromycin, ciprofloxacin (high-level), penicillin G (intermediate), tetracycline (intermediate).

 

Neisseria   gonorrhoeae

NCTC 13818

WHO V

Resistant to; azithromycin (high-level), ciprofloxacin (high-level), penicillin G   (penicillinase-producing), tetracycline.

 

Neisseria   gonorrhoeae

NCTC 13819

WHO W

Resistant to; cefixime (low-level),   ciprofloxacin (high-level), penicillin G, tetracycline.

 

Neisseria   gonorrhoeae

NCTC 13820

WHO X

Resistant to; azithromycin (intermediate). Cefixime (high-level), ceftriaxone (high-level), ciprofloxacin (high-level), penicillin G.

 

Neisseria   gonorrhoeae

NCTC 13821

WHO Y

Resistant to; azithromycin, cefixime (high-level), ceftriaxone (high-level) and ciprofloxacin   (high-level), penicillin G (intermediate), tetracycline.

 

Neisseria   gonorrhoeae

NCTC 13822

WHO Z

Resistant to; azithromycin, ceftriaxone (low-level), cefixime (high-level) and ciprofloxacin (high-level), penicillin G, tetracycline.

 

 

 

British Society for Antimicrobial Chemotherapy (BSAC)

Method for Antimicrobial Susceptibility Testing (Version 14 January 2015)

Control strains with a resistance mechanism

Organism

NCTC® Strain Reference

Characteristics

Other collection number

Bacteroides fragilis

NCTC 9343

Susceptible

ATCC 25285; CN 6300;

DSM 2151

Bacteroides thetaiotaomicron

NCTC 13706

Susceptible

 -

Clostridium perfringens

NCTC 8359

Susceptible

ATCC 12915; CN 3335

Enterococcus faecalis

NCTC 12697

Susceptible

ATCC 29212; WDCM 00087

Escherichia coli

NCTC 12241

Susceptible (β-lactamase negative)

ATCC 25922; DSM 1103; NCIMB 12210; WDCM 00013

NCTC 10418

Susceptible

ATCC 10536; CN 6455; DSM 682; NCIB 8879;

Escherichia coli

NCTC 11560

TEM-1 ß-lactamase producer

 -

Haemophilus influenzae

NCTC 11931

Susceptible

 -

Haemophilus influenzae

NCTC 12699

Resistant to ß-lactams (ß-lactamase-negative)

ATCC 49247

Neisseria gonorrhoeae

NCTC 12700

Low level chromosome mediated resistant to penicillin

ATCC 49226

Pasteurellamultocida

NCTC 8489

Susceptible

 -

Pseudomonas aeruginosa

NCTC 12903

Susceptible (contains inducible AmpC β-lactamase)

ATCC 27853; WDCM 00025

NCTC 10662

Susceptible

ATCC 25668; CCUG 1423; DSM 46358; WDCM 00114

Staphylococcus aureus

NCTC 12981

Susceptible (β-lactamase negative)

ATCC 25923; CIP 76.25; DSM 1104; JCM 2413; WDCM 00034

NCTC 6571

Susceptible

ATCC 9144; CN 6457; NCIB 6571; NRRL-B314; WDCM 00035

Staphylococcus aureus

NCTC 12493

MecA positive, methicillin resistant

WDCM 00212

Streptococcus pneumoniae

NCTC 12977

Low level resistant to penicillin (altered penicillin binding protein)

ATCC 49619; CIP 104340

 

 

 

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