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CHO-K1-AC-free

CHO-K1-AC-free

Catalogue No.

13080801

Cell Line Name

CHO-K1-AC-free

Cell Line Description

This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, EX-CELL® ACF CHO medium (Sigma C5467)

Characteristics

Tissue of Origin

Ovary

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Subculture Routine

A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen, this can take 3-4 weeks. Use of Ultra Low Attachment flasks will help maintain the culture in suspension. Once established the culture should be split approximately every 4-5 days, maintaining a cell density between 3-9x105 cells/ml.  Recommended Growth conditions: 5% CO2; 37°C

Culture Medium

EX-CELL® ACF CHO medium (Sigma C5467) + 8mM Glutamine. When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.

Growth Mode

Aggregates in suspension

Additional Info

Depositor

Dr. E. Penn, Culture Collections, Public Health England, Porton Down, Salisbury, SP4 0JG, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None Specified By Depositor

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1-AC-free (ECACC 13080801).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.