|Cell Line Name:||MDCK II|
|Keywords:||Canine Cocker Spaniel Kidney|
|Cell Line Description:||MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.|
|Subculture Routine:||Split sub-confluent cultures (70-80%) every 2-3 days; seeding at 1-3 x 10,000cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C,|
|Culture Medium:||MEM + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.|
|Depositor:||Professor Gerrit van Meer, Dept. of Membrane Enzymology, Centre for Biomembranes and Lipid Enzymology, H.R Kruytgebouw N607, Padualaan 8, 3584 CH Utrecht, The Netherlands.|
|References:||Hansson, G.C.,Simons, K and Van Meer G (1986) EMBO 5: 483-489, Louvard D (1980) PNAS 77; 4132-4136|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
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