|Cell Line Name:||VCaP|
|Keywords:||Human Prostate Cancer Metastasis|
|Cell Line Description:||This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.|
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.|
|Culture Medium:||DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Karyotype:||Refer to reference (Korenchuk et al., 2001)|
|Depositor:||Professor Kenneth Pienta, Departments of Urology and Internal Medicine, University fo Michigan Medical Center, The University of Michigan, Ann Arbor, Michigan, USA|
|References:||Korenchuk et al., (2001) VCaP, a cell-based model system of human prostate cancer. In Vivo 15, 163-8.|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
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