Culture Collections

General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: VCaP

Catalogue No.: 06020201
Cell Line Name: VCaP
Keywords: Human Prostate Cancer Metastasis
Cell Line Description: This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
Species: Human
Tissue: Prostate metastasis
Morphology: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19

Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
Culture Medium: DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Refer to reference (Korenchuk et al., 2001)
Receptors: Androgen receptor
Depositor: Professor Kenneth Pienta, Departments of Urology and Internal Medicine, University fo Michigan Medical Center, The University of Michigan, Ann Arbor, Michigan, USA
Originator: Yes
Country: USA
References: Korenchuk et al., (2001) VCaP, a cell-based model system of human prostate cancer. In Vivo 15, 163-8.
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: No
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)

  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

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