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CHP-134

CHP-134

Catalogue No.

06122002

Cell Line Name

CHP-134

Cell Line Description

A neuroblastoma cell line established from a tumour of the left adrenal area of an 18-month-old male patient. The patient was previously treated with vincristine, cyclophosphamide, dimethyltriazenoimidazole carboxamide, adriamycin, and irradiation. The cells grow as adherent colonies consisting of neuroblast-like cells forming dense mounds. Individual cells radiate from the mounds which reach confluency after routine passaging for several months. This cell line has been reported to have tyrosine hydroxylase activity.

Characteristics

Products

Neural enzymes: choline acetyl-transferase, acetylcholinesterase, tyrosine hydroxylase

Tissue of Origin

Adrenal (neuroblastoma)

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 9,13
D5S818: 11,12
D7S820: 9
D13S317: 8,11
D16S539: 11,13
TH01: 9,9.3
TPOX: 8
vWA: 18,19

Karyotype

Not specified

Disease

Neuroblastoma

Culture Conditions

Cell Type

Neuronal

Subculture Routine

Split confluent cultures 1:2 to 1:10 every 2-3 days, seeding at 2-4x10,000 cells/cm². Use gentle agitation to dislodge cells, trypsin/EDTA should not be necessary as cells only adhere loosely; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr Peter White, Department of Pediatrics, University of Pennsylvania and Civic Centre Blvd. Philadelphia PA 19104-4318 USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Schlesinger et al., (1976) Establishment and Characterization of Human Neuroblastoma Cell Lines. Cancer Research, Sept. vol 36 (9 pt1) 3094-3100

Bibliography

Not Available

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: CHP-134 (ECACC 06122002).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.