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GPNT

GPNT

Catalogue No.

09061601

Cell Line Name

GPNT

Cell Line Description

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

General Info

Species

Rat

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I

Products

GPNT cells express tight junction proteins

Tissue of Origin

Brain

Morphology

Fusiform

Applications

GPNT is a cell model for cerebral endothelium used to study the blood-brain barrier.

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 10hrs.

Culture Medium

Hams F10 + 10% Heat-inactivated FCS + 2mM Glutamine + 2ng/ml basic fibroblast growth factor + 80µg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10µg/cm² from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to ammonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

Growth Mode

Adherent

Additional Info

Depositor

Professor John Greenwood, University College London Institute of Opthalmology, University College London, Bath Street London ECIV 9EL.

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Greenwood et al., (1996) SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology 71: 51-63. PMID: 8982103.

Bibliography

Regina et al., (1999) Dexamethasone regulation of P-glycoprotein activity in an immortalized rat brain endothelial cell line, GPNT. Journal of Neurochemistry 73: 1954-1963. PMID:10537053.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: GPNT (ECACC 09061601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.