|Cell Line Name:||JU77|
|Keywords:||Human malignant mesothelioma|
|Cell Line Description:||This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140|
|Morphology:||Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"|
|Subculture Routine:||Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.|
|Culture Medium:||RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS|
|Depositor:||Richard Lake - University of Western Australia, Australia|
|References:||Manning LS, Whitaker D, Murch AR, Garlepp MJ, Davis MR, Musk AW, Robinson BW (1991) Int J Cancer Jan 21;47(2):285-90|
|Research Council Deposit:||No|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.