|Cell Line Name:||AtT20|
|Keywords:||Mouse LAF1 pituitary gland tumour|
|Cell Line Description:||Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.|
|Morphology:||Small round cells growing as large aggregates|
|Growth Mode:||Aggregates in suspension|
|Subculture Routine:||Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding density of approximately 3 x 105/ml is recommended. 5% CO2; 37°C. Proliferation is enhanced by incubation on a shaking platform.|
|Culture Medium:||Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.|
|Products:||Adrenocorticotropic hormone (ACTH)|
|Depositor:||Obtained from ATCC|
|References:||FURTH J, GADSEN EL, UPTON AC 1953 ACTH secreting transplantable pituitary tumors. Proc Soc Exp Biol Med. 84(1):253-4 PMID: 13121002.|
|Additional Bibliography:||BUONASSISI V, SATO G, COHEN AI.1962 Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci U S A. 48:1184-90 PMID: 13874682.|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
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