|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||AtT20|
|Other Collection No.:||ATCC CCL 89|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: AtT20 (ECACC 87021902)|
|Keywords:||Mouse LAF1 pituitary gland tumour|
|Cell Line Description:||Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.|
|Tissue of Origin:||pituitary|
|CellType:||Small round cells growing as large aggregates|
|Growth Mode:||Aggregates in suspension|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||This cell line loses viability when frozen and hence the expected viable cell count when resuscitated from frozen stock may be only 40 -50%. To sub-culture allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding density of approximately 3 x 105/ml is recommended. 5% CO2; 37°C. Proliferation is enhanced by incubation on a shaking platform.|
|Culture Medium:||Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.|
|Depositor:||Obtained from ATCC|
|References:||FURTH J, GADSEN EL, UPTON AC 1953 ACTH secreting transplantable pituitary tumors. Proc Soc Exp Biol Med. 84(1):253-4 PMID: 13121002.|
|Additional Bibliography:||BUONASSISI V, SATO G, COHEN AI.1962 Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci U S A. 48:1184-90 PMID: 13874682.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Please confirm your country of origin from the list below.