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UK Health Security Agency

COLO 201

COLO 201

Catalogue No.

87091201

Cell Line Name

COLO 201

Cell Line Description

COLO 201, COLO 205 (ECACC Catalogue 87091208) and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Characteristics

Products

Carcinoembryonic antigen (CEA) less than produced by COLO 205

Tissue of Origin

Colon

Morphology

Clumps of spherical cells in suspension

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 10,13
D7S820: 9,10
D13S317: 10
D16S539: 12,13
TH01: 8,9
TPOX: 11
vWA: 15

Karyotype

Triploid modal no. 71-78

Disease

Adenocarcinoma

Culture Conditions

Subculture Routine

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x10⁴ cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Semple TU, Quinn LA, Woods LK, Moore GE 1978.Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38(5):1345-55 PMID: 565251.

Bibliography

Trainer DL et al., 1988 Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int J Cancer. 41(2):287-96 PMID: 3338874.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 201 (ECACC 87091201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.