|Cell Line Name:||THP 1|
|Keywords:||Human monocytic leukaemia|
|Cell Line Description:||
Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.
Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line.
Please note: We routinely resuscitate and culture this cell line with success, however, some laboratories have found this cell line difficult to establish following resuscitation from a frozen ampoule. Our Quality Control (QC) Department strongly recommend that this line is supplied as a Growing culture whenever possible e.g. to locations within or near the European Union. We routinely resuscitate and maintain this cell line growing in our laboratory to enable us to supply growing cultures quickly. If however, you still wish to receive the cell line Frozen we can supply but our normal replacement Policy will not apply should you encounter resuscitation difficulties.
We recommend, if you are in the European Union (where we can deliver growing cultures), you select a Growing culture.
|Subculture Routine:||If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr J Clarke, AVRI, Pirbright|
|References:||Int J Cancer 1980;26:171; Cancer Res 1982;42:1530; J Immunol 1983;131:1882|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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