|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||H69|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: H69 (ECACC 91091802)|
|Keywords:||Human Caucasian lung small cell carcinoma|
|Cell Line Description:||This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.|
|Tissue of Origin:||lung|
|Growth Mode:||Aggregates in suspension|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Maintain cultures between 6x10,000 to 5 x100,000 cells/ml; 5% CO2; 37°C.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr R I Freshney, CRC Department of Medical Oncology, University of Glasgow|
|References:||Carney DN, Gazdar AF, Bepler G, Guccion JG, Marangos PJ, Moody TW, Zweig MH, Minna JD. 1985 Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res.45(6):2913-23 PMID: 2985257.|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Please confirm your country of origin from the list below.