Culture Collections

General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: H69

Catalogue No.: 91091802
Cell Line Name: H69
Keywords: Human Caucasian lung small cell carcinoma
Cell Line Description: This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.
Species: Human
Tissue: lung
Growth Mode: Aggregates in suspension
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Subculture Routine: Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO2; 37°C.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Aneuploid
Depositor: Dr R I Freshney, CRC Department of Medical Oncology, University of Glasgow
Originator: No
Country: UK
References: Carney DN, Gazdar AF, Bepler G, Guccion JG, Marangos PJ, Moody TW, Zweig MH, Minna JD. 1985 Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res.45(6):2913-23 PMID: 2985257.
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: No
Release Conditions: No

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats Price
  • Frozen
    Re-stocking *
    £360.00
  • Growing
    Re-stocking *
    £514.50
  • DNA-5µg (100ng/µl)
    Re-stocking *
    £115.00
  • RNA-5µg (100ng/µl)
    Re-stocking *
    £175.00
  • cDNA-20µl
    Re-stocking *
    £145.00
Quantity
*Re-stocking: contact for availability






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