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General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: A2780cis

Catalogue No.: 93112517
Cell Line Name: A2780cis
Keywords: Human ovarian carcinoma
Cell Line Description: This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (ECACC catalogue no. 93112519) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media every 2-3 passages. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (ECACC catalogue no. 93112520), has been isolated from the same parental line A2780.
Species: Human
Tissue: ovary
Morphology: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,13
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16

Subculture Routine: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 1x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Culture Medium: RPMI 1640 + 2mM Glutamine + 1µm cisplatinum + 10% Foetal Bovine Serum (FBS) (cisplatinum only necessary every 2-3 passages).
Karyotype: Modal no. 46
Depositor: Dr T H Ward, Cell Culture Unit, Patterson Laboratories, Christie Hospital, Manchester
Originator: Yes
Country: UK
References: Cancer Res 1987;47:414; Cancer Res 1988;48:5713
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: No
Release Conditions: No

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats Price
  • Frozen
    In Stock
    £475.00
  • Growing
    In Stock
    £629.50
  • DNA-5µg (100ng/µl)
    In Stock
    £115.00
  • RNA-5µg (100ng/µl)
    In Stock
    £175.00
  • cDNA-20µl
    In Stock
    £145.00
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