Culture Collections

General Cell Collection Detail

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General Cell Collection: DH82

Catalogue No.: 94062922
Cell Line Name: DH82
Keywords: Canine Monocyte-macrophage
Cell Line Description: Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.
Species: Dog
Tissue: macrophage
Morphology: Macrophage
Growth Mode: Suspension
Subculture Routine: Cells are semi-adherent, i.e. some cells grows in suspension, whilst most attach to the surface and may flatten. Attached cells should be removed with 0.02% EDTA. Split sub-confluent cultures (70-80%)  i.e. seeding at 2-4x10,000 cells/cm²; 5% CO2; 37°C.  
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Karyotype: Not specified
Receptors: FC-gamma receptors
Depositor: Obtained from ATCC
Originator: No
Country: USA
References: Wellman ML, Krakowka S, Jacobs RM, Kociba GJ 1988 A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev Biol.24(3):223-9. PMID: 3350786.
Additional Bibliography: Gröne A, Fonfara S, Baumgärtner W. 2002 Cell type-dependent cytokine expression after canine distemper virus infection. Viral Immunol. 15(3):493-505. PMID: 12479398.
Patents: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 5,192,679
Research Council Deposit: No
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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