|Cell Line Name:||DH82|
|Cell Line Description:||Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.|
|Subculture Routine:||Cells are semi-adherent, i.e. some cells grows in suspension, whilst most attach to the surface and may flatten. Attached cells should be removed with 0.02% EDTA. Split sub-confluent cultures (70-80%) i.e. seeding at 2-4x10,000 cells/cm²; 5% CO2; 37°C.|
|Culture Medium:||EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).|
|Depositor:||Obtained from ATCC|
|References:||Wellman ML, Krakowka S, Jacobs RM, Kociba GJ 1988 A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev Biol.24(3):223-9. PMID: 3350786.|
|Additional Bibliography:||Gröne A, Fonfara S, Baumgärtner W. 2002 Cell type-dependent cytokine expression after canine distemper virus infection. Viral Immunol. 15(3):493-505. PMID: 12479398.|
|Patents:||This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 5,192,679|
|Research Council Deposit:||No|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
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