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UK Health Security Agency

DMS 79

DMS 79

Catalogue No.

95062824

Cell Line Name

DMS 79

Cell Line Description

DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, beta-endorphin, 17-beta-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.

Characteristics

Tissue of Origin

Lung

DNA profile (STR Profile)

Amelogenin: X, Y
CSF1PO: 10
D5S818: 10
D7S820: 9,11
D13S317: 11
D16S539: 12
TH01: 8
TPOX: 8
vWA: 18

Karyotype

Aneuploid

Applications

Human lung tumour model, carcinogenesis studies.

Disease

Small Cell Cancer

Culture Conditions

Cell Type

Carcinoma

Subculture Routine

Maintain cultures between 3 - 9 x 100,000 cells/ml; 5% CO₂; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore be done by dilution. During acclimation to your laboratory , it is wise to count DMS 79 cells when cultures are split as there is apparent sensitivity to cell number.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Aggregates in suspension

Additional Info

Depositor

Prof OS Pettengill and Prof. GD Sorenson, Dartmouth Medical School, Lebanon, NH

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer 1980;45:905

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: DMS 79 (ECACC 95062824).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.