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UK Health Security Agency

NCI-H510A

NCI-H510A

Catalogue No.

96020944

Cell Line Name

NCI-H510A

Cell Line Description

The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar amd associates in 1982 from an adrenal metastasis in an adult, 56-year-old, male Caucasian patient. The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. The line does not exhibit any gross structural DNA abnormalities. The cells express elevated levels of four biochemical markers of small cell lung carcinoma (SCLC) i.e. neuron specific enolase, the brain isoenzyme creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. This cell line is tumourigenic in nude mice (the cells form transplantable tumours with typical small cell carcinoma histology).

Characteristics

Tissue of Origin

Lung (adrenal metastasis)

Karyotype

Hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes common to all cells. These include t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+.

Disease

Small Cell Cancer

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. This cell line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks. Adherent cells can be detached using 0.05% trypsin or trypsin/EDTA. However, retain suspension cells separately prior to using trypsin on the adherent cells.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent/aggregates in suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Takahashi et al., 1989. P53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494. Carney et al.,1985.Establishment and identification of small cell lung cancer cell lines having classic and variant features. Can Res 45:2913

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: NCI-H510A (ECACC 96020944).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.