Culture Collections

General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: NCI-H69/LX4

Catalogue No.: 96042329
Cell Line Name: NCI-H69/LX4
Keywords: Human Caucasian small cell lung carcinoma, drug-resistant
Cell Line Description: NCI-H69/LX4 has been established by exposure of the parent line, H69 (ECACC catalogue no. 91091802) to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.
Species: Human
Tissue: Lung
Growth Mode: Aggregates in suspension
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Subculture Routine: Split sub-confluent cultures (70-80%) by repeated pipetting of aggregates or scraping; 5% CO2; 37°C
Culture Medium: RPMI + 2mM Glutamine + 0.4µg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Depositor: Dr P Twentyman / Dr K Wright, MRC Clinical Oncology Unit, Cambridge, UK
Originator: Yes
Country: UK
References: Br J Cancer 1992;65:335; Br J Cancer 1986;53:529
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: Yes
Release Conditions: No

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)

  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through your local distributor.

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