Culture Collections

New Cell Lines

We have an active programme to acquire new cell lines for our catalogue. On this page we list recent additions.  You can also find these lines within the extensive Cell Line and Hybridoma collection listings.

 

 

HipSci Panels

April 2017

For new users of our iPSC resource, we have composed two panels of lines from the HipSci iPSC collection that we would recommend. These lines have been selected based on their high pluritest scores, low CNV’s, comprehensively characterised assay data, and their ability to differentiate.

 

These lines are also available to order as individual lines.

 

Click here to view the panels

 

Nd ES

January 2017

A mouse embryonic stem cell (ES) line (Nd) containing a novel fluorescent reporter (VNP) to investigate the temporal dynamics of Nanog expression in mouse and to dissect the lineage potential at different Nanog expression levels. This novel reporter ES cell line allows the accurate monitoring the dynamic expression of the transcription factor Nanog in the pluripotent state. The cell line will be a valuable tool to obtain quantitative measurements of global gene expression in pluripotent mouse ES cells in different states, allowing a detailed molecular mapping of the pluripotent state.

Catalogue number Cell line name Keywords
16010601 Nd ES mouse embryonic stem cells, Nanog expression, pluripotency

 

Anti-oestrogen resistant cell lines

January 2017

Ximbio (Cancer Research Technology, UK) has announced the availability of anti-oestrogen resistant breast cancer cell lines from the research of Dr Anne Lykkesfeldt at the Danish Cancer Society. These cell lines have been developed, characterised and published for a number of subtypes of the parental human breast cancer cell lines MCF-7 and T47D, which demonstrate resistance to both first-line (tamoxifen or aromatase inhibitor) and second-line (fulvestrant) hormone-dependent breast cancer treatments. Both parental cell lines, MCF-7 and T47D, are dependent on oestrogen for growth and as a result the oestrogen receptor (ER) has become a key target for clinical therapies. The anti-oestrogen resistant cell lines have the potential to be utilised as novel models for, understanding molecular mechanisms of drug-resistance in cancer, identifying biomarkers of therapeutic response and developing new anti-cancer drugs.

 

ECACC General Cell Collection
8 items found, displaying all items.
Catalogue No. Cell Line Name Keywords
16022512 T47D/S2 Human, Breast, Cancer, Oestrogen receptor
16022509 MCF7/TAMR-7 Breast cancer, tamoxifen-resistant
16022513 T47D/TR-1 Breast carcinoma, tamoxifen resistant, oestrogen receptor
16022523 MCF7/ExeR-4 Breast cancer, Exemestane resistance
16022524 MCF7/LetR-1 Breast cancer, Letrozole resistant
16022519 MCF7/AnaR-4 Breast cancer, MCF7, Anastrozole resistance
16022501 MCF7/S0.5 Human, Breast, Cancer, Oestrogen receptor, MCF7
16022506 MCF7/182R-6 Breast cancer, fulvestrant resistant


 

CHO lines

A number of new CHO cell line variants, developed in the laboratory of Dr Mike Clark at Cambridge University have been deposited with the ECACC General Collection, by Cambridge Enterprise. These CHO cell lines express different allotypes of Fc gamma RIIA receptors on the cell surface and are useful for the investigating the binding of recombinant antibodies to human Fc receptors. Cell lines expressing human and macaque Fc receptors have been developed since these are both useful to pre-clinical research. The cell lines may be used for the measurement, by flow cytometry, of monomeric IgG binding or complexed  IgG binding to receptors. They are also used for studying the association of Fc receptor bearing cell lines with red blood cells coated with IgG. They are used in assays that include the target cell of the IgG, comparing natural IgG constant regions, mutated IgG constant regions and those with altered carbohydrate profiles. The cell lines may also be used as a release assay for batches of clinical grade antibodies since binding will be sensitive to aggregates or changes in carbohydrate composition.

Catalogue number Cell line name Keywords
15042901  CHO-K1.Cl6 CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158V, allotype, human IgG , IgG binding
15042902  CHO-K1.Cl7 CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158F, allotype, human IgG , IgG binding
15042903  CHO-K1.Cl-0204 CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131R, allotype 
15042905  CHO-K1.Cl-0205 CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131H, allotype, human IgG , IgG binding
15042907  CHO-K1.Cl-0206 CHO-K1, FcγRIIb , Fc gamma receptor 2b
15042908  CHO-K1.Cl-0235 CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Pan troglodytes, chimpanzee
15042909  CHO-K1.Cl-0236 / CHO-K1.Cl-0239 CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Cercocebus torquatus, red-crowned mangabey, papio anubis, olive baboon
15042910  CHO-K1.Cl-0237 CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca fascicularis (Cynomolgus macaque)
15042911  CHO-K1.Cl-0238 CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca mulatta (Rhesus macaque)
15042912  CHO-K1.Cl-0270 CHO-K1, non-human primate FcγRIIb , Fc gamma receptor 2b, Macaca mulatta, Rhesus macaque, Macaca fascicularis, Cynomolgus macaque

 

 

L929/A (an Adriamycin-resistant cell line)

This adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue no. 85011425) to increasing concentrations of adriamycin in vitro. L929/A cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Catalogue No. Cell line name Keywords
14112101 L929/A Mouse C3H/An connective tissue Adriamycin resistant

 

 

 

A Reporter Cell Line for Use in Autophagy Studies

A stable cell line expressing EGFP-tagged LC3 (rat) in background of HEK293 cell line. This reporter cell line can be used to monitor induction of autophagy after amino acid starvation or rapamycin treatment as well as autophagosome formation. Also, this cell line provides a useful tool for the study of autophagy using GFP-LC3 as a standard assay read out by both immunofluorescence and biochemical methods.

Catalogue No. Cell line name Keywords
14050801 HEK293A GFP-LC3 Human embryonic kidney, autophagy, HEK293, EGFP-rat LC3, reporter cell line, ATG8, microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)

 

 

HeLa-Mitotrap ‘Knock-sideways’ Cell Lines for Investigating Protein Function

Two new HeLa-Mitotrap cell lines, which can be used to determine the function of a protein, by rapidly inactivating the protein of interest through rapamycin induced rerouting to the mitochondria, a ‘knock sideways’ approach, are now available from ECACC. This method of protein inactivation, which relocates proteins away from their site of action rather than destroying them, is more rapid than knockout or knock down strategies.

 

 

Catalogue No.

Cell line name

                     
Keywords
15042201 HeLa-Mitrotrap Human cervix carcinoma, knocksideways, rapid protein inactivation by rerouting to mitochondria for functional studies, genetically modified
15042203 HeLa-Mitrotrap Ap1g1-FKBP Human cervix carcinoma, knocksideways, rapid protein inactivation by rerouting to mitochondria for functional studies, adaptor protein (Ap1g1), genetically modified

 

 

ULK cell lines for autophagy research

A series of mouse embryonic fibroblasts with different capability for  uncoordinated (Unc)-51-like kinase 1 and 2 (ULK1 and ULK2) activity, which are known to play a critical role during the activation of autophagy, are now available from PHE’s European Collection of Authenticated Cell Cultures (ECACC).  Autophagy is the mechanism by which a cell degrades unnecessary or dysfunctional cellular components and has been implicated in several medical scenarios such as cancer, neurodegeneration and immunity related disorders.  The cell lines were deposited by Dr Sharon Tooze of Cancer Research UK.

 

Catalogue No.

Cell line name

ULK 1 and 2 status        

Immortalisation method             

14050808

ULK1/2 WT MEF (SIM)

Wild type

Spontaneously immortalised

14050804

ULK1/2 WT MEF (SV40)

Wild type

SV40-immortalised

14050806

ULK1 KO MEF (SIM)

ULK1 knock-out

Spontaneously immortalised

14050807

ULK1 KO MEF (SV40)

ULK1 knock-out

SV40-immortalised

14050810

ULK2 KO MEF (SIM)

ULK2 knock-out

Spontaneously immortalised

14050805

ULK2 KO MEF (SV40)

ULK2 knock-out

SV40-immortalised

14050803

MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)

Double knock-out

Spontaneously immortalised

14050802

MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40)

Double knock-out

SV40-immortalised

 

 

 

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