Culture Collections

Mycoplasma testing

We can test your cell lines, cell culture media and reagents to determine if mycoplasma is present. Many researchers choose to use our service to ensure they are working with mycoplasma free cell cultures.  The effects of mycoplasma contamination on cell lines can be wide ranging and are often underestimated. These include:

  • Alteration of growth rate
  • Induction of morphological changes
  • Chromosomal aberrations
  • Altered cell metabolism
  • Decreased viability upon resuscitation of frozen ampoule.

All laboratories carrying out cell culture should test for mycoplasma contamination on a regular basis. We recommend that a combination of methods is used to determine whether a sample is free of mycoplasma.  This is because the different methods have different levels of detection sensitivity and some strains of mycoplasma do not grow in vitro. These can be detected by DNA stain and PCR.

 

Mycoplasma

 

 

 

 

 

 

 

 

Electron micrograph of Mycoplasma infected cell line


Three different mycoplasma detection methods are routinely used by our scientists at the European Collection of Authenticated Cell Cultures (ECACC), namely:

 

  PCR more information
  Indirect Hoechst Stain more information
  Culture Isolation more information

 

Mycoplasma detection methods and prices:

 

Name of Test

Catalogue Number

Price

Sensitivity

Time Taken

Species Range

Meets FDA points to consider in   characterisation of cell lines used to produce biologicals (1993)

Mycoplasma Detection by PCR

ECSR33

£72.50

Low/   Medium

1 day

Majority

No

Mycoplasma Detection by Indirect Hoechst Stain

ECSR60

£125

Medium

2-3 days

All

Yes

Mycoplasma Detection by Culture Isolation

ECSR61

£175

High

3-4 weeks

Majority

Yes

All three Mycoplasma Detection methods:   Mycoplasma Detection by PCR, Indirect   Hoechst Stain and Culture Isolation

ECSR39

£300

High

3-4 weeks

All

See above for each test

Sample preparation from frozen ampoule 

 ECSR30

 £75

 N/A

 

N/A

 

 N/A

 

N/A

 

 

 

 

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.