The glossary is an alphabetical list of acronyms and terms related to our products.
The transfer or transplantation of cells, with or without dilution, from one culture vessel to another. It is understood that any time cells are transferred from one vessel to another, a certain portion of the cells may be lost and therefore, dilution of cells, whether deliberate or not, may occur. This term is synonymous with the term 'subculture'.
The number of times the cells in the culture have been subcultured or passed. In descriptions of this process, the ratio or dilution of the cells should be stated so that the relative cultural age gap can be ascertained. This term is not synonymous with population doubling.
Phosphate Buffered Saline(calcium and magnesium free)
Phosphate buffered saline
Penicillin Control and Immunology Section, Food andDrug Administration, Washington DC, USA
Collection of Mycoplasmas of Dr D G ff Edwards, formerly at Burroughs Wellcome Research Laboratories, Beckenham, Kent UK
The expressed characteristics of a cell or cell culture. This includes the morphology, markers, products secreted and all other physical attributes.
Public Health Laboratory Service, England and Wales , now subsumed within the Health Protection Agency
A description of the number of chromosome sets included in a cell. eg. 2n - diploid/normal cells, 3n - triploid, 4n - tetraploid, etc.
Phorbol Myristate Acetate
The total number of population doublings of a cell line or strain since its initiation in vitro . A formula to use for the calculation of 'population doublings' in a single passage is: number of population doublings = Log10(N/N0) X 3.33 where: N= number of cells in the growth vessel at the end of a period of growth N0= numberof cells plated in the growth vessel. It is best to use the number of viable cells or number of attached cells for this determination. Population doublings level is synonymous with 'cell generation time'.
Personal Protective Equipment
The way in which ECACC prioritises its cell line stocks. Price code A reflects those cell lines ordered most often and so the banks are larger and more cryogenic storage space is assigned to them. Other price codes consist of cell lines that are ordered less often and so stocks are smaller and more labour intensive.
A culture started from cells, tissues or organs taken directly from organisms. A primary culture may be regarded as such until it is successfully subcultured for the first time, when it becomes a 'cell line'.
Refers to an increase in viable cell numbers in a population or when a single cell doubles.
Propagating strain for staphylococcal bacteriophages
This describes the condition where the number of chromosomes in a cell is diploid but, as a result of chromosomal rearrangements, the karyotype is abnormal and linkage relationships may be disrupted.
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