Culture Collections

Culture Collections news

Read about our new products, collaborations, upcoming events and find out about latest scientific developments and announcements. We welcome your feedback and comments - please email culturecollections@phe.gov.uk

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Using cells in virology small news

Using cell lines in Virology

The development and production of vaccines requires large quantities of viruses or bacteria. Bacteria are easily cultured, providing they have a suitable growth medium and conditions. However, viruses infect living cells in order to replicate; resulting in difficulties for laboratories.

 

July 2019

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S.adjame small newsRare Salmonella serotype deposited into the NCTC

Earlier this year, scientists from Public Health England’s Gastrointestinal Bacteria Reference Unit and collaborators described the first known outbreak of Salmonella Adjame which occurred in 2017 in London, UK. The strains are now deposited in our collection as NCTC 14246NCTC 14247 and NCTC 14248.

 

July 2019

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NCPV 20 Small News

20 years serving the virology community 

The National Collection of Pathogenic Viruses was established in 1999, with funding from the Wellcome Trust, for the benefit of developing and testing of vaccines and antiviral compounds. Our remit is to identify, preserve and provide relevant pathogenic viruses for the scientific community. 

 

July 2019

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Exploring the pathology of less prominent speciesC. Diff Small News

There are approximately 200 validly described species in the genus Clostridium. Among them are multiple human and animal pathogens. Naturally, the genus also contains species whose pathogenic potential and clinical significance remains understudied. 

 

July 2019

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U2OS Capture Small News

U-2 OS Cell Line Profile

U-2 OS is one of the earliest described cancer derived cell lines obtained from a moderately differentiated bone. Recently, the cell line has shown itself to be useful as a successful transfection host. This combined with absence of any detectable adenovirus infection makes the cell line a useful expression platform in virology.

 

July 2019

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EBiSC2

EBISC survey 

The European Bank for induced pluripotent Stem Cells (EBiSC) is carrying out a survey to better understand users’ iPSC needs. If you use or intend to use iPSCs, help us understand your needs by completing the survey. 

 

 

July 2019 

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UK NEQAS

Importance of UK NEQAS External Quality Assurance (EQA)

UK NEQAS is an independent non-profit organisation with nearly 50 years’ experience in the provision of EQA to clinical laboratories. EQA is an educational tool that enables participants to examine, monitor and improve their own laboratory performance. 

 

May 2019

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N. gonorrhoeae

Increasingly antimicrobial resistant Neisseria gonorrhoeae added to the NCTC & World Health Organisation reference strain panel

The first globally reported Neisseria gonorrhoeae strain to exhibit high-level ceftriaxone and azithromycin resistance has recently been made available in our collection as NCTC 14208.

 

May 2019

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silver

NCTC and the science of small silverware

Microorganisms are used to to understand the properties of microscopic particles. It is clear that scientists are using bacterial pathogens from the National Collection of Type Cultures (NCTC) to test nanoparticle applications in fighting infectious diseases. This was demonstrated after NCTC curators reviewed up to 100 studies published in 2018.

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May 2019

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Figure 3. Theoretical pH ranges for different culture media in increasing CO2 levels in a 37°C humidified incubator. CO2 Concentration and pH Control in the Cell Culture Laboratory

Most cell culture laboratories will have a humidified Carbon Dioxide (CO2) incubator set to 5%, however it is not always obvious why our cultures need this amount of CO2, nor what the acceptable tolerances are for the ranges in concentration. 

 

 April 2019

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EBiSC2

EBiSC2

The European Bank for induced pluripotent Stem Cells launches a second project phase to become a self-sustainable repository for human induced pluripotent stem cells (hiPSC), extend the existing cell catalogue, and offer additional hiPSC-related services. 

 

 

 April 2019

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SEM cell line

ECACC: A Dynamic Culture Collection for Disease Research

Twenty new cell lines are now available to researchers which include numerous cancer cell lines relating to colorectal, breast and gastric cancers, treatment resistant cancer cell lines, immortalised mouse embryonic fibroblast cell lines and hybridomas.

 

 February 2019

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Zika­ imageHow culture collections can assist responses to emerging disease

Diagnosis of viral infections is often achieved by testing clinical samples using molecular methods such as PCR and sequencing, or serology. 

 

 

 January 2019

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New NCPV viruses in 2018Adenovirus

In 2018, eight new viral strains from five families were made available through the NCPV online catalogue.  

 

 

 

January 2019

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BacteriophageNCTC bacteriophage collection and repository

The UK Standards for Microbiology Investigations (UK SMI) have issued a new virology document, V53: Screening and monitoring for hepatitis E infection. 

 

 

January 2019

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UKSMI V53 HEV document newsUK SMI: New HEV screening document 

The UK Standards for Microbiology Investigations (UK SMI) have issued a new virology document, V53: Screening and monitoring for hepatitis E infection. 

 

 

January 2019

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Ian Freshney

Dr. R. Ian Freshney (1938-2019): A tribute

Dr. R Ian Freshney passed away on 6 January 2019. He was a distinguished scientist and will be much missed by the cell culture community.

 

 

January 2019

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Streptococcus ICONStreptococcus pneumoniae: New deep sequenced reference genome available A new detailed reference genome accompanied with its own novel genome browser (produced using a combination of state-of-the-art sequencing and bioinformatics technologies) for the NCTC 7466 descendent strain NCTC 14078 / D39V Streptococcus pneumoniae has recently been published in Nucleic Acids Research.

 

November 2018

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NCTC in China ICONNCTC's partners in Beijing It’s always exciting for NCTC microbiologists to visit other Culture Collections and a recent opportunity to drop in on the China Center of Industrial Culture Collection (CICC)was no exception.

 

 

 

November 2018

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NCTC in Nigeria ICONNCTC: the national collection supporting global health NCTC are proud to support the partnership between the PHE International Health Regulation (IHR​) Strengthening project and Nigeria’s Centre for Disease Control (NCDC​).

 

 

 

November 2018

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DNA ICON

The Next Generation Sequencing (NGS) service at Public Health England have sequenced over 100,000 microbial genomes. NCTC E.coli K12 DNA is the control organism of choice.

 

 

 

November 2018

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Herpes Simplex ICONHerpes simplex virus type 1 panel A team at the National Reference Laboratory at Public Health England isolated a series of HSV-1 viruses from immunocompromised patients undergoing antiviral therapy, and phenotyped them for antiviral susceptibility.

 

 

November 2018

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Flask and card ICON

Advances in molecular pathology: Cell line authentication Advances in Molecular Pathology, a new Elsevier journal, was launched earlier this month. This first issues contains four articles on cell line missidentification.

 

 

November 2018

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M Robertson ICONDr. Muriel Robertson FRS (1883-1973) for the new £50 design The National Collection of Type Cultures has nominated Dr. Muriel Robertson to feature on the Bank of England’s £50 note

 

 

 

November 2018

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Ebola ICON

 

PHE-MOHS Ebola Biobank The PHE-MOHS Ebola Biobank holds approximately 9955 residual clinical samples collected during the West Africa Ebola outbreak.  The biobank is an open resource and is available to bona fide scientists undertaking health related research to inform public health strategy relating to Ebola.      

September 2018

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Artist in residence ICON

NCTC welcomes Anna Dumitriu as artist in residence  Pioneering BioArtist Anna Dumitriu has joined NCTC as artist in residence and will be exploring the extensive collection to develop artworks inspired by and incorporating NCTC's historic microbes

 

 

August 2018

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NCTC 3000 Sanger ICON

NCTC in the media: Scientists map genetic codes of 3000 dangerous bacteria Professor Julian Parkhill’s group at the Wellcome Sanger Institute (WSI) and Pacific Biosciences featured together with the NCTC team in multiple media articles in June 2018 after we reached the important milestone of having sequenced DNA from 3000 NCTC bacteria.

 

August 2018

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Klesiella pneumoniae ICON

Research in focus: Hyper virulent Klebsiella pneumoniae Hyper virulent Klebsiella pneumoniae is an emergent pathogen requiring harmonised surveillance between clinical microbiology laboratories. Further study into its epidemiology and its ability to acquire and retain antimicrobial resistance characteristics is of significant importance.

 

August 2018

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Buffalo ICON

Buffalo Pox In 2005, pustular lesions were noticed on the foot of a burns patient in Karachi, Pakistan. Similar lesions subsequently appeared on at least 19 other patients in the city’s burns units. As part of the investigation into the infection, biopsies were sent to Public Health England Porton for specialist testing.

 

August 2018

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Antimicrobial resistance - ICON

NCTC 10455 – a reference control for a bla-FRI variant Healthcare settings are responding to the emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) by introducing routine rectal screening for those patients at risk of CPE carriage.

 

 

August 2018

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Measles ICONThe measles virus is highly contagious and infects millions of individuals worldwide. It causes serious disease in over 10 million children and accounts for about 120,000 deaths each year.

 

 

July 2018

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Abstract staircase ICON

Calling all Technology Transfer Offices New and modified cell lines generated by universities and not for profit organisations are key in enabling ECACC to expand and keep its cell line collections relevant to the current needs of life science research. Working closely together has provided many benefits for both partners 

 

July 2018

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Chris Morris ICONMemoirs of ECACC Chris Morris was a longstanding member of the ECACC team who retired in 2017. In this piece he reminisces on his time in ECACC, the path of his career and the people he has worked with along the way.

 

 

 

July 2018

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TS Webinar - Growing Pains ICONFree webinar - Growing Pains: Cell Culture Challenges and Best Practices This webinar covered several topics including standardisation of training, steps for improving reproducibility of culture setup and maintenance, the importance of authenticating cell lines and the challenges posed by novel cell culture systems such as iPSC, organoids and complex 3D cell culture.

 

 July 2018

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ECACC Workshop ICONECACC's Advanced Cell Culture Workshop: An overview The objectives of the workshop were to provide a forum where industry and academic experts could present on developing models and systems that recapitulate human biology in the lab, increasing the physiological relevance of experiments whilst reducing reliance on animal models.

 

July 2018

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Bovine Intestinal Organoid ICONApplying good cell culture practice to novel systems The emergence of induced pluripotent stem cells (iPSC), organoids and complex 3D cell culture offer huge potential for increasingly physiologically relevant in vitro models but also offer a novel set of challenges for the implementation of good cell culture practice.

 

July 2018

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Roland Nardone, PhD ICON

We were sorry to learn that Dr Roland Nardone passed away on 20th June 2018. Roland had a passionate belief that science must be reproducible and became a leading pioneer in alerting the biomedical scientific community to the widespread global problem of misidentified cell lines in life science research. He devoted himself to promoting and resolving this issue from 2005.

 

 

June 2018

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NCPV 60246 Schmallenberg virus particles, TEM - ICON

Arborvirus new leaflet download Several emerging and re-emerging neglected diseases and zoonoses (infections that can pass between animals and humans) are caused by arboviruses, with vaccines available for only a few of these infections.

 

 

June 2018

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Adenovirus ICON

Three Species D Adenovirus Isolated from Patients with AIDS Whole Genome Sequenced

Three species D adenoviruses isolated from the faeces of AIDS patients in the UK, have been whole genome sequenced and are now available from NCPV.

 

 

May 2018

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Locked genome ICONNCPV 500: An Electronic Resource for Mining Viral Genomes

NCPV 500 is collaboration between Public Health England and the Wellcome Trust Sanger Institute (WTSI) to produce 500 viral genomes from PHE’s National Collection of Pathogenic Viruses (NCPV) using the Illumina sequencing platform. 

 

May 2018

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Bacteriophage ICONThe rise and rise of bacteriophages

Read our update on NCTC 3000 the collaborative bacterial Whole Genome Sequencing (WGS) project between Public Health England (PHE), Pacific BioSciences (PacBio) and the Wellcome Trust Sanger Institute (WTSI).

 

 

May 2018

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NCTC 3000 Update ICON

An Update on NCTC3000: A Type Culture Reference Genome Project

Read our update on NCTC 3000 the collaborative bacterial Whole Genome Sequencing (WGS) project between Public Health England (PHE), Pacific BioSciences (PacBio) and the Wellcome Trust Sanger Institute (WTSI).

 

May 2018 

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Proteomics ICONThe Role of Proteomics in the National Collection of Type Cultures

Proteomics studies proteins in protein complexes, organelles or organisms and represents a challenging and complex discipline of biology. NCTC is fortunate to have two proteomics scientists dedicated to applying proteomic technologies to NCTC strains.

 

May 2018

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Gut anatomical ICONIn vitro Modelling of the Gut Brain Axis and Microbiome for Neurodegenerative Diseases 

The human microbiome is a complicated ecosystem. The gut microbiome is essential to healthy neuronal development and it has been shown that germ-free upbringing of mice induces significant permanent neurodevelopmental deficit.

 

May 2018

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ECACC STEM Alvetex ICON

Advanced cell culture systems for investigating microbe-host interactions

 

3D cell culture technology can assist in the study of microbe-host interactions in a setting which better represents in vivo physiological conditions.

 

April 2018

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ECACC Workshop 2018 - Advanced Cell Culture ICONECACC one day workshop - Advanced Cell Culture: A Practical Approach

Register now for your space at our one day workshop in London. 

 

 

April 2018

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NCTC AMR ICON

Antibiotic resistance - a significant challenge to public health microbiology

Read our ResearchGate post on how NCTC bacterial strains can be used to better understand antibiotic resistance and enhance antibiotic research.

 

 

March 2018

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NCPV Usutu virus ICON

Usutu virus: A new addition to the NCPV catalogue

Usutu is an old world flavivirus of increasing notoriety. The virus was first isolated in South Africa in 1959 and was named after the Usutu River in Swaziland. The typical Usutu life cycle involves both mosquitos and birds, and infection within mammals remains uncommon.

 

March 2018

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NCPF Aspergillus fumigatus ICON

The Aspergillus fumigatus genome-wide knockout collection

On the 1st January 2018 a team led by Dr Mike Bromley at the University of Manchester embarked on a Wellcome Trust funded project named COFUN that aims to generate knockout mutants for all of the coding genes in the human pathogen Aspergillus fumigatus.

January 2018

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NCPF research 2017 ICON

Breaking the mould (and the yeasts) in biomedical research – NCPF in 2017

Candida and Aspergillus are the most prevalent fungal infections in hospitals but other species cannot be neglected. A research group in Spain have described a PCR-assay to help obtain a more rapid and accurate identification of Saprochaete capitata.

 

January 2018

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NCPF - Candida - ICON

NCPF in Research: Update on the emerging pathogen Candida auris in the UK

After its first description in 2009, C. auris had rapidly emerged as an important cause of nosocomial infections across several continents, with large outbreaks in hospitals in South Africa, India, parts of North and South America, Spain and the UK.

 

January 2018

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NCTC Anaerobes for research ICONAnaerobes for research

Anaerobes are often perceived as less important in clinical microbiology compared to their aerobic counterparts. The complexities of infections caused by anaerobes are still not fully understood 

 

 

January 2018

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NCTC Authenticating Anaerobes ICONAuthenticating anaerobes

Within NCTC, great care is taken when authenticating all bacteria within the collection. Fastidious anaerobes pose a unique challenge.

 

 

January 2018

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NCTC ICON

Blood, sweat and sequencing – NCTC in 2017

A collaborative study by researchers in Thailand and the UK identified a novel antibacterial peptide derived from Crocodylus siamensis haemoglobin that causes bacterial death. 

  

 

January 2018

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NCPV Citations 2017 ICON

From Antivirals to Zika: NCPV in 2017

A study by researchers based in the UK and US used an NCPV strain of human rhinovirus (HRV strain 1B) to demonstrate a potential role for cathelicins as part of a therapeutic strategy for rhinovirus infection. 

  

 

January 2018

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NCPV nucleic acid ICONViral Nucleic Acids  

Viruses contain nucleic acid as RNA or DNA. These molecules possess all the genetic information of the virus. Research involving the use of viral nucleic acid products, are becoming increasing popular.

 

 

January 2018

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NCPV Chikungunya ICON

Chikungunya virus 

Chikungunya virus causes a painful, crippling disease, and is transmitted by infected mosquitoes. The name is derived from a native language of Tanzania, and refers to the stooped, contorted appearance of sufferers with severe joint pain.

 

 

January 2018

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ECACC Word on the street - ICON100 tiny steps - ECACC in research 2017

Microwaves have been used with some success as cancer therapy; scientists in Japan used an ECACC human breast cancer cell line to provide evidence to support the use normothermic microwave irradiation in clinical cancer treatment; just one example of the role of ECACC cell lines in improving our understanding of non-communicable diseases.

 

January 2018

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Pipetting - ICONECACC Training Course

There are still a limited amount of spaces on our Fundamentals of Cell Culture: Key Principles and Practice training course in March. Register now to secure your space.

 

 

January 2018

 

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UKBRCN ICONUK Biological Resource Centre Network

The UKBRCN is a network of UK biological collections including PHE's four Culture Collections. The group meets annually and aims provide support to collections and their users on topics such as the Nagoya Protocol, and also aims to foster active collaborative links within the biological resource community.

December 2017

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NCPV PhD ICONAre you starting a PhD in microbiology?

Get your research off to the best start. Don’t lose precious time trouble-shooting reagents. By obtaining biological materials from the Culture Collections of Public Health England, you can move straight to addressing the exciting questions. 

 

December 2017

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ECACC Nucleic Acid ICONVariety and applications of Nucleic Acid products

Genetic researchers are now learning more about how individual genes work and what they do and study the role that variations in genes play in disease. ECACC provides nucleic acids in a range of  formats from its many hundreds of human cell lines to help researchers with their studies.

 

November 2017

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ECACC CEF cryopreservation ICON

Optimisation of cryo-preservation of chick embryo fibroblast cells 

Chick embryo fibroblasts (CEF) are a useful cellular reagent in virology as they support the growth of many human and animal pathogenic viruses. This study has helped ECACC establish reproducible cell banks of CEF that can be used directly for virus propagation and titration without the need for reverting to primary tissue preparation. 

November 2017

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NCTC Shigella record ICONNCTC and Antimicrobial Medicine: Past, present and future

The 18th of November marked European Antibiotic Awareness Day. NCTC has a proud history of supporting the development of antimicrobial medicine and continues to provide biological materials used in the harmonized measurement of antimicrobial resistance (AMR) and bio-resources for modern AMR research.

November 2017

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ECACC media ICON

Growth media: not all culture media is the same

In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition, the culture environment should be stable in terms of pH and temperature. Over the last 30 years various defined basal media types have been developed and are now available commercially.

November 2017

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Nagoya World Map ICON

Implementation of the Nagoya Protocol: an update

Life science research and microbiological diagnostics rely on access to global genetic resources.  In 2014, the Nagoya Protocol came into force. It aims to provide a framework for legal clarity to be achieved for providers and users of genetic resources regarding fair access and sharing of any benefits arising from use.

November 2017

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ECACC celebrating 10yrs of HiPSC ICON

Celebrating Ten Years of Human Induced Pluripotent Stem Cells

2017 marks the 10th anniversary of the first human induced pluripotent stem cells (HiPSC) and the year of ECACC’s global launch of its collections of over 1,000 HiPSC cell lines (HipSci and EBiSC).

 

November 2017

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NCTC Shigella record ICON

NCTC: We can’t be experts in everything – so can you contribute?

From Abiotrophia adiacens to Zymomonas mobilis, NCTC  microbiologists work with nearly 6000 bacterial strains from more than 900 distinct species in at least 83 different families. The methods used to authenticate the strains ranges from simple microscopy to whole genome sequencing.

 

November 2017

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EBiSC neuron ICON

Webinar - iPSCs: the future of disease research. The development of human iPSC technology offers researchers the ability to more accurately generate physiologically relevant models of disease and normal tissues in the laboratory. Advances in iPSC generation have allowed laboratories to make their own cell lines; however, researchers rarely have the resources needed to establish stocks, undertake quality control and share their own de novo iPSC cell lines.

Read more...

October 2017

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NCTC 100th IBMS placement student ICON

In August 2017 Eleanor Hill, became the 100th placement student within Public Health (PHE) to complete the Registration Training Portfolio for the IBMS Certificate of Competence. Eleanor, Biomedical Science undergraduate from De Montfort University, spent a year in the NCTC laboratory gaining experience in microbiological techniques and working in a professional setting in addition to working towards the Portfolio.

Read more...

October 2017

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NCTC strain equivalence ICON

Thousands of living species are newly described every year and in the microbial world the tree of life is expanding dramatically due to the identification of previously unknown lineages through genome analysis.  For bacteria, the first isolate of a confirmed new species, the type strain, must be deposited within two internationally recognised culture collections

Read more...

October 2017

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NCTC picture logo ICONNew species of bacteria are constantly being discovered and described. These new species may differ only slightly or much more significantly from those already described and can be isolated from a variety of sources, including from clinical specimens.

Read more...

 

 

October 2017

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Book and Pen ICON

A recent study published by Horbach and Halffmann (2017) reports on the number of ‘contaminated’ papers in scientific literature in which misidentified cell lines are used. The number of articles referring to misidentified cell lines is still increasing and a ‘fair and reasonable notifications system’ is therefore required to raise awareness of this issue.

Read more...

October 2017

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Crowd - B&W ICON

Informed consent - ensuring clinical samples are obtained ethically Researchers working with biological samples taken from both animals and humans need to know that the material they are working with has been obtained legally, ethically and with due consideration for the wellbeing, privacy and dignity of the donor.

Read more...

October 2017

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EBiSC: New panel of heart disease-specific iPSC ICON

Current therapeutic strategies for a variety of inherited cardiac conditions are mostly symptomatic. Cardiac myocytes (CMs) derived from disease-specific iPSCs represent a novel in vitro system with an unprecedented potential for generating new mechanistic insight into diseases pathophysiology and enabling enhanced assessment of drug efficacy and safety.

Read more...

October 2017

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NCTC 3000 strains ICON

NCTC 3000 is a collaborative Whole Genome Sequencing (WGS) project that was established in 2013 between Public Health England (PHE), the Wellcome Trust Sanger Institute (WTSI) and Pacific Biosciences (PacBio). Search the new list of sequenced NCTC strains to find genomes that interest you.

 

Read more...

September 2017

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Pipetting - ICON

Webinar: Cell Line Solutions for Assay Development and Bioproduction Cultivating and managing productive cell cultures is of critical importance to the biopharmaceutical industry. Recent discoveries using stem cells, advances in cell based therapeutics, and the rapid expansion of the biosimilar market has investigators searching for technologies that will improve their research workflows.

 

Read more...

September 2017

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NCPF in research: MALDI ToF - ICON

NCPF in research: MALDI-ToF mass spectrometry for the identification of eumycetoma agents and related fungi The PHE Mycology Reference Laboratory (MRL) has undertaken studies relating to fungi that cause black grain mycetoma. Black grain eumycetoma is a chronic fungal infection and many different fungal species are known to cause it including Madurella species, Falciformispora species and Trematosphaeria grisea.

 

Read more...

August 2017

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NCTC Corynebacterium ICON

NCTC is reviewing the toxigenic status of the Corynebacterium strains it curates. Provision of authenticated reference cultures along with comprehensive phenotypic data is an essential part of supporting public health efforts, research and promoting safety in the scientific community.

 

 

Read more...

August 2017

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NCTC animals vegetables minerals ICON

A first glance through the 2017 publications citing NCTC bacteria reveals a fascinating insight into the reference strains’ continued importance in emerging microbial developments. Recent focus on microbiomes has changed ideas about the role of microorganisms in human and animal health and disease, and antimicrobial resistance remains a global challenge. 

 

Read more...

August 2017

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Ice hockey black ICONNCPV has recenetly added a number of Mumps virus strains of differing genotypes to the collection. Earlier this year a Canadian news channel reported that five Canadian ice hockey players had been side-lined due to mumps. One player’s jaw was so swollen that he assumed it was broken. In the UK mumps is a notifiable infection and high profile outbreaks are often associated with festivals and certain high risk populations.

Read more...

August 2017

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Annotated electrophrenogram ICONECACC has recently launched a new service for human cell line authentication by STR profiling. The process is simple and quick; cells are spotted onto an FTA card and sent for analysis by post at ambient temperature. To help researchers understand the STR profile and report, ECCAC have created several resources on STR profile interpretation.

 

Read more...

26 July 2017

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Spectral karyotyping of human lymphoma cell line ICONDepositing a cell line with ECACC: the strengths of quality control, storage and distribution Cell lines are increasingly critical tools for life science research and as concerns over scientific reproducibility grow, it is essential that they are of known origin, and they are valid in terms of identity, freedom from microbial contamination and function. ECACC maintains secure master and working banks of cryopreserved stocks of the cell lines in its collection.

Read more...

27 June 2017

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NCTC heater-cooler ICON

NCTC is working to provide authenticated reference strains of Mycobacterium chimaera. The type strain is now available as NCTC 13781.Until fairly recently M.chimaera was a low profile microorganism in the sphere of human infection. However, this changed in 2014 when six cases of severe infection were reported in cardiac surgery patients in Zurich. 

 

Read more...

27 June 2017

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NCTC in research Mycobacterium ICON

Many researchers use NCTC strains during the development, evaluation and validation of laboratory methods.  A team at Queen’s University, Belfast, UK, used NCTC Mycobacteria strains to evaluate an assay for the rapid detection of Mycobacterium avium subsp. paratuberculosis (MAP) in milk. MAP causes Johne’s disease (JD), a chronic enteric wasting disease that affects domestic ruminants. 

 

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27 June 2017

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Tubes - ICON

Making cell lines more physiologically relevant for toxicology testing: how sugar may not help the medicine go down Thanks to their accessibility, ease of use and suitability for high throughput analysis, cancer derived human cell lines are useful laboratory tools for the screening of the effectiveness and toxicology of potential new drugs.  

 

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22 June 2017

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Gorilla in London Zoo - ICON

Conservation through cell culture ECACC currently supplies  a number of cell lines which were established from species listed under the Convention on International Trade in Endangered Species of Wild Flora and Fauna - CITES. Scientists can be reassured that the use of these cell lines means that no additional animals have been taken from the wild, or from captive bred colonies to provide biological samples for research use.

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22 June 2017

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Stefan - ICON

Upcoming webinar with Stefan Przyborski More sophisticated models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays.  Methods that are relatively straightforward to use and that recreate the organized structure of real tissues will become valuable research tools for use in discovery, validation studies, and modeling disease. 

 

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06 June 2017

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NCTC: HG3 organisms (Mycobacterium tuberculosis) ICON

Hazard Group 3 bacteria in the NCTC collection In the UK, biological agents are classified into four Hazard Groups (HG1-4). Similar categorisations of biological agents are also seen in other parts of the world. The majority of bacteria within the NCTC collection fall within the HG2 category, however the collection also holds over 500 strains of HG3 bacteria.

 

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26 May 2017

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Nicola 1 - ICON

ECACC: Supporting life science research and technology transfer ECACC has worked closely with UK Research Councils, charities and universities’ Technology Transfer offices to increase the range and relevance of the cell lines available from ECACC for life science research. Working closely with a single contact within a research establishment enables us to fast-track new cell lines into ECACC, making them available for the wider research community as quickly as possible.  

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25 May 2017

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iPSC enhanced - ICON

10 Years of Human Induced Pluripotent Stem Cells: From Theory to Accessible Research Tools In 2006 Professor Shinya Yamanaka first presented the work of his team showing that just four transcription factors were required to transform a skin cell into a pluripotent stem cell. This technology is now called reprogramming and the cells generated from its use are termed induced pluripotent stem (iPS) cells.

 

Read more...

25 May 2017

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NCPF Colin Campbell ICONExophiala campbellii: a new fungal species. The Mycology Reference Laboratory recently undertook a study of Exophiala species contained within the NCPF collection to see they could distinguish between them using MALDI-ToF technology. Read Dr Elizabeth Johnson’s account of how this investigation led to the identification of a new species.

 

 

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24 May 2017

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Andy at cabinet - ICONCell culture is a critical tool for biological science research. For over 30 years ECACC has been instrumental in the education of cell culture scientists through its regular and internationally renowned cell culture training courses and it is estimated that over this time around a thousand delegates have received direct training at the hands of ECACC staff. 

 

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15 May 2017

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NCPF Emarellia ICONNCPF in research: A new species responsible for black grain mycetoma has been identified. Phylogenetic analyses of 31 isolates that were historically classified as Madurella greisea has given rise to the identification of a new species. The clinical isolates from two international collections were all associated with cases of black-grain mycetoma infection in humans.

 

Read more...

03 May 2017

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Tubes ICON

Cell culture: useful websites and publications. There is a growing need for standardisation to enable reproducibility within life science research. As our reliance on in vitro methodologies increases, it is important that researchers have access to resources that help guide workflow and experimental design, and provide basic and up to date guidance to help overcome common pitfalls and challenges encountered in cell culture.

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19 April 2017

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FTA2 ICON

STR profiling using FTA cards for human cell line authentication. ECACC can now offer an STR profiling cell line authentication service where cells are simply spotted onto a special (FTA) card and sent for profiling at ambient temperature. This negates the need to extract DNA from cell pellets and removes the cold chain distribution required for sending cell pellets on dry ice.

 

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19 April 2017

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EBiSC neuron ICON

EBiSC to develop next generation Alzheimer's disease iPSC lines. Gene editing in induced pluripotent stem cells (iPS cells) has within the last couple of years emerged as a valuable tool. One of the most powerful applications is the generation of isogenic lines for patient specific disease modeling.

 

 

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19 April 2017

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NCTC Dynamic Collection ICONNCTC must endure as a dynamic collection of bacteria, accessible and significant to the biomedical scientific community. With this objective in mind we have recently added five new strains that can be used as positive controls for detecting beta-lactamase genes.

 

 

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19 April 2017

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NCTC Murray ICONMurray, microbes, and me - The life of the microbiologist and clinician Professor Everitt Murray is the focus of a recent article in The Microbiology Society’s publication Microbial Genomics. The article by Kate Baker charts Murray’s career and involvement with NCTC.

 

 

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07 April 2017 

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Microbiologist 3D - ICON

3D culture systems are used in many biological science disciplines including cell biology, immunology, tissue engineering and cancer and stem cell researchThis technology can assist in the study of virus-host interactions in a setting which better represents in vivo physiological conditions; tissue-like constructs which are similar to their equivalents in vivo are present allowing for more in depth, accurate and relevant investigations to occur.

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29 March 2017 

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NCTC lyophilisation ICON

Lyophilisation, also known as freeze-drying, is a preservation method achieved through dehydration. The process was developed initially in 1906 and the technique currently applied by NCTC is an adaptation of the method used during World War II to preserve and transport blood serum. 

 

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15 March 2017 

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Microscope - ICONSetting up an inverted phase contrast microscope for tissue culture Often microscope users are unaware of how to set up an inverted microscope and either suffer from low quality images or adopt a trial an error approach to try to improve results.  Understanding how to set up the microscope properly can save time and greatly increase the quality of images and data generated from cell cultures

 

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13 March 2017 

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IWD2017 - ICONInternational Women's Day 2017 Historically women have experienced greater barriers to the same opportunities afforded to men and hostility from the scientific establishment (among others), breeding a range of problems. For the first time in its history the majority of the National Collection of Type Cultures’ (NCTC) senior scientists and curators are women. We pay tribute to the women who shaped and contributed to one of the world’s oldest working bacterial collections as it exists today.

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08 March 2017 

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NCPV Brochure - ICONYou can now learn about the authenticated viral strains and services provided by the National Collection of Pathogenic Viruses (NCPV) in our new brochure. The brochure helps virologists and quality control scientists understand the range of viral strains relevant to human health that are available from NCPV, their applications and the benefits of depositing their own viral strains in the collection. 

 

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02 March 2017 

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Fleming ICONApproximately one fifth of the bacteria in NCTC are cultures of type strains, which are the strains on which the description of a species is based.  A bacterial species invariably includes multiple strains that are of common origin and show more similarity to each other than to any other strain. We could consider the type strains to be the most important strains in NCTC because they are the points of reference that other strains must be compared with when determining which species they belong to.

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15 February 2017

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Salmonella XLD ICONSalmonella infection is a major global cause of foodborne disease and is frequently transmitted as a result of the consumption of contaminated foods of animal origin; contamination in animal feed is known to be a contributing factor. Rapid methods for identifying Salmonella-free samples are being developed which have the potential to reduce the turnaround time for negative results from three to two days.

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15 February 2017

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Reproducibility - ICON

Reproducibility in pre-clinical life science research. Reproducibility forms the foundation of meaningful scientific research and is an issue which is causing increasing concern. The causes are complex and include lack of validation of key biological reagents and reference material (including cell lines), poor study design, inadequate data analysis and reporting and a lack of robust laboratory protocols. 

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14 February 2017

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ECACC CHOK1 Flask - ICON

CHO cell lines expressing Fcγ receptors – an in vitro tool for antibody based drug discovery. New CHO cell line variants, expressing different classes of Fc gamma receptors (FcγRs) have been deposited exclusively into the ECACC General Collection by Cambridge Enterprise Limited, UK. The variant lines were developed in the laboratory of Dr. Mike Clark at Cambridge University and include five cell lines that express three different human FcγRs including allotypes of FcγRIIa and FcγRIIIa.

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14 February 2017

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ECACC Word on the street - ICON

ECACC cell lines in research: 2016

A review of 80 scientific publications citing the use of ECACC cell lines, published on line during 2016, emphasises the value of cell lines as tools in our quest to understand and combat human diseases. The use of 88 different ECACC cell lines was cited by authors affiliated to organisations in 36 countries, with significant focus on the pathogenesis and treatment of human cancers.  

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13 February 2017

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Aedes mosquito on skin - ICONYellow fever virus is an arbovirus of the Flaviviridae family, pathogenic to humans, and transmitted by mosquitoes (Aedes and Haemogogus species). It is endemic in tropical areas of Africa and South America and although vaccination is available, take-up is low in some endemic areas. In 2015/2016 a large outbreak occurred in Angola and the Democratic Republic of Congo, and in Brazil this year, 47 cases have been confirmed and there have been 45 deaths.

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26 January 2017

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Zika­_CDC_Cynthia Goldsmith ICONNCPV’s global impact in 2016. During 2016 NCPV was at the forefront of the global response to the outbreak of Zika virus that has affected people in up to 70 countries across South America, the Caribbean and the Western Pacific Region since October 2013. We supplied live Zika virus strains or RNA from NCPV Zika strains to 50 different groups of scientists in 16 countries. NCPV also made publically available the first Zika virus strain with extensive genome sequence data isolated from a semen sample

  

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25 January 2017

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NCTC Public Engagement in 2016 ICONHannah McGregor, Quality Control Supervisor at NCTC, tells us about her public engagement activities in 2016.

I am very lucky to work for NCTC - not only because I work with some extraordinary and historical bacteria on a daily basis, but I get the opportunity to share my passion for microbiology with members of the public and potential future scientists.

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19 January 2017

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NCTC New bacterial strains ICONNCTC: New bacterial strains in 2016. We made a record number of bacterial strains available from the NCTC online catalogue in 2016. In total, 98 strains from 20 families were added , a quarter of which were Type strains. Many were included in the NCTC_3000 whole genome sequencing project under taken with the Sanger Institute and PacBio.

 

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19 January 2017

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FBS Screening - ICONECACC offers several thousand cell lines to researchers; the majority of these require serum for growth, which presents the problem of deciding on a suitable supply. Many laboratories batch test sera by setting up multiple cultures, and then performing cell counts at fixed time points. This is time consuming and requires  duplicate counts at every time point to provide sufficient accuracy. At ECACC we have developed an automated method using an IncuCyte live cells analysis system.  

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19 January 2017

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NCPF M. romeroi - ICONNCPF in Research: Phaeohyphomycosis cyst associated with M. romeroi infection. Typically these phaeohyphomycocis infections are caused by fungal species such as Exophiala, Alternaria, Cladophialophora, Phialophora or Curvularia/Bipolaris species. Medicopsis species has also been implicated.  This case study reports the occurrence of an infection at an intravenus cannula site.

  

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11 January 2017

 

CO2 Concentration and pH Control in the Cell Culture Laboratory

Most cell culture laboratories will have a humidified Carbon Dioxide (CO2) incubator set to 5%, however it is not always obvious why our cultures need this amount of CO2, nor what the acceptable tolerances are for the ranges in concentration. 

 

July 2019

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