Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Acholeplasma laidlawii

NCTC Number: NCTC 10116
Current Name: Acholeplasma laidlawii
Original Strain Reference: PG 8
Other Collection No: ATCC 23206; PG 8; SEWAGE A
Previous Catalogue Name: Acholeplasma laidlawii
Type Strain: Yes
Family: Acholeplasmataceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1295523
16S rRNA Gene Sequence: >gb|AF294996|ATCC 23206|Acholeplasma laidlawii 16S-23S ribosomal RNA intergenic spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| ctttctaaggagaaa... >gb|U14905|PG8 ATCC 23206|Acholeplasma laidlawii PG8 ATCC 23206 16S ribosomal RNA gene,partial sequence.| agagtttgatcctgg... >gb|AY740437|ATCC 23206|Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| cgcccgtcaaaccac... >gb|AY740436|ATCC 23206|Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| cgcccgtcaaaccac...
23S rRNA Gene Sequence: >gb|AF294996|ATCC 23206|Acholeplasma laidlawii 16S-23S ribosomal RNA intergenic spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| ctttctaaggagaaa... >gb|AY740437|ATCC 23206|Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| cgcccgtcaaaccac... >gb|AY740436|ATCC 23206|Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| cgcccgtcaaaccac...
Bibliography: EDWARD D G FF & FREUNDT E A 1970 J GEN MICROBIOL 62 1; EDWARD D G FF &
Extended Bibliography: showhide Show bibliography
Ref #: 14473
Author(s): Artiushin,S.;Duvall,M.;Minion,F.C.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of mycoplasma strain ISM1499 and its assignment to the Acholeplasma oculi strain cluster
Volume: 45
Page(s): 104-9
Year: 1995
Keyword(s): GENBANK/M23932 GENBANK/U14904 GENBANK/U14905 GENBANK/U14906 Acholeplasma/*classification/genetics Base Composition Base Sequence Molecular Sequence Data Mycoplasma/*classification/genetics Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/chemistry/genetics Sequence Homology, Nucleic Acid
Remarks: A mycoplasma strain designated ISM1499 was used to develop a mycoplasma genetic system (G. G. Mahairas and F. C. Minion, J. Bacteriol. 171:1775-1780, 1989; G. G. Mahairas, C. Jian, and F. C. Minion, Gene 93:61-65, 1990), but phenotypic inconsistencies led to the conclusion that this organism had been classified incorrectly as a member of the species Mycoplasma pulmonis. Studies were initiated to determine the proper taxonomic position of ISM1499, and on the basis of the results of our genetic analysis, this strain was assigned to the Acholeplasma oculi strain cluster. The base composition of strain ISM1499 was identical to the base composition of A. oculi 19L, but not to the base composition of Acholeplasma laidlawii PG8 (28.3 and 30.7 mol% G+C, respectively). The taxonomic position of ISM1499 was examined by performing a parsimony analysis with 16S ribosomal DNA sequence data, and the results were compared with previous phylogenetic reconstructions. Our results indicated that ISM1499 is more closely related phylogenetically to A. oculi 19L than to A. laidlawii PG8 and JA1. Heterogeneity in the 16S ribosomal DNA sequences of A. oculi 19L and ISM1499 and in the 16S ribosomal DNA sequences of A. laidlawii PG8 and JA1 may indicate that unusual dissimilarities occur in the 16S ribosomal DNA sequences of members of the genus Acholeplasma.
URL: 7857790
Ref #: 14410
Author(s): Kong,F.;James,G.;Gordon,S.;Zelynski,A.;Gilbert,G.L.
Journal: Appl Environ Microbiol
Title: Species-specific PCR for identification of common contaminant mollicutes in cell culture
Volume: 67
Page(s): 3195-200
Year: 2001
Keyword(s): GENBANK/AF294989 GENBANK/AF294990 GENBANK/AF294991 GENBANK/AF294992 GENBANK/AF294993 GENBANK/AF294994 GENBANK/AF294995 GENBANK/AF294996 Cell Line/*microbiology DNA Primers DNA, Ribosomal Spacer/genetics Humans Molecular Sequence Data Mollicutes/*classification/genetics/*isolation & purification Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sensitivity and Specificity Sequence Analysis, DNA Species Specificity
Remarks: Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and Acholeplasma laidlawii are the members of the class Mollicutes most commonly found in contaminated cell cultures. Previous studies have shown that the published PCR primer pairs designed to detect mollicutes in cell cultures are not entirely specific. The 16S rRNA gene, the 16S-23S rRNA intergenic spacer region, and the 5' end of the 23S rRNA gene, as a whole, are promising targets for design of mollicute species-specific primer pairs. We analyzed the 16S rRNA genes, the 16S-23S rRNA intergenic spacer regions, and the 5' end of the 23S rRNA genes of these mollicutes and developed PCR methods for species identification based on these regions. Using high melting temperatures, we developed a rapid-cycle PCR for detection and identification of contaminant mollicutes. Previously published, putative mollicute-specific primers amplified DNA from 73 contaminated cell lines, but the presence of mollicutes was confirmed by species-specific PCR in only 60. Sequences of the remaining 13 amplicons were identified as those of gram-positive bacterial species. Species-specific PCR primers are needed to confirm the presence of mollicutes in specimens and for identification, if required.
URL: 11425741
Ref #: 95481
Author(s): Kim,K.S.;Ko,K.S.;Chang,M.W.;Hahn,T.W.;Hong,S.K.;Kook,Y.H.
Journal: FEMS Microbiol Lett
Title: Use of rpoB sequences for phylogenetic study of Mycoplasma species
Volume: 226
Page(s): 299-305
Year: 2003
Keyword(s): GENBANK/AY191418 GENBANK/AY191419 GENBANK/AY191420 GENBANK/AY191421 GENBANK/AY191422 GENBANK/AY191423 GENBANK/AY191424 GENBANK/AY191425 GENBANK/AY191426 GENBANK/AY191427 GENBANK/AY191428 GENBANK/AY191429 GENBANK/AY191430 GENBANK/AY191431 GENBANK/AY191432 GENBANK/AY191433 GENBANK/AY191434 GENBANK/AY191435 GENBANK/AY191436 GENBANK/AY191437 GENBANK/AY191438 GENBANK/AY191439 GENBANK/AY191440 GENBANK/AY191441 GENBANK/AY191442 GENBANK/AY191443 Amino Acid Sequence DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*chemistry/*genetics Genes, Bacterial Molecular Sequence Data Mycoplasma/classification/*genetics *Phylogeny RNA, Ribosomal, 16S/genetics Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Ureaplasma/genetics
Remarks: rpoB sequences encoding the beta-subunit of RNA polymerase were determined in 26 Mycoplasma species for phylogenetic study. The portion of rpoB DNA used in this study showed a high degree of variation in terms of size and sequence among species. The rpoB phylogenies inferred from amino acid and nucleotide sequences were used to divide the mycoplasmas into two groups, a 'pneumoniae group' and a 'hominis group', which was consistent with the result from 16S rDNA sequence analysis. However, phylogenetic relationships within these groups differed in the two gene trees, which were supported by the incongruence length difference (ILD) test. This indicates that multiple gene sequences must be applied to infer accurate phylogenetic relationships among the mycoplasmas. The rpoB sequence, and especially the deduced amino acid sequence, offers a good alternative marker.
URL: 14553926
Ref #: 95435
Author(s): Volokhov,D.V.;George,J.;Liu,S.X.;Ikonomi,P.;Anderson,C.;Chizhikov,V.
Journal: Appl Microbiol Biotechnol
Title: Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing
Volume: 71
Page(s): 680-98
Year: 2006
Keyword(s): GENBANK/AY729927 GENBANK/AY729928 GENBANK/AY729929 GENBANK/AY729930 GENBANK/AY729931 GENBANK/AY729932 GENBANK/AY729933 GENBANK/AY729934 GENBANK/AY729935 GENBANK/AY729936 GENBANK/AY736029 GENBANK/AY736030 GENBANK/AY736031 GENBANK/AY736032 GENBANK/AY736033 GENBANK/AY737010 GENBANK/AY737011 GENBANK/AY737012 GENBANK/AY737013 GENBANK/AY738726 GENBANK/AY738727 GENBANK/AY738728 GENBANK/AY738737 GENBANK/AY738738 GENBANK/AY738739 GENBANK/AY740425 GENBANK/AY740426 GENBANK/AY740427 GENBANK/AY740428 GENBANK/AY740429 GENBANK/AY740430 GENBANK/AY740431 GENBANK/AY740432 GENBANK/AY740433 GENBANK/AY740434 GENBANK/AY740435 GENBANK/AY740436 GENBANK/AY740437 GENBANK/AY741671 GENBANK/AY741672 GENBANK/AY741673 GENBANK/AY741674 GENBANK/AY741675 GENBANK/AY744936 GENBANK/AY744937 GENBANK/AY744938 GENBANK/AY744939 GENBANK/AY744940 GENBANK/AY744941 GENBANK/AY744942 GENBANK/AY753215 GENBANK/AY753216 GENBANK/AY755603 GENBANK/AY755604 GENBANK/AY755605 GENBANK/AY755606 GENBANK/AY757364 GENBANK/AY762638 GENBANK/AY762639 GENBANK/AY762640 GENBANK/AY762641 GENBANK/AY762642 GENBANK/AY762643 GENBANK/AY762644 GENBANK/AY765210 GENBANK/AY765211 GENBANK/AY765212 GENBANK/AY765213 GENBANK/AY765214 GENBANK/AY765215 GENBANK/AY765216 GENBANK/AY766086 GENBANK/AY766087 GENBANK/AY766088 GENBANK/AY766089 GENBANK/AY766090 GENBANK/AY766091 GENBANK/AY766092 GENBANK/AY768810 GENBANK/AY770622 GENBANK/AY770623 GENBANK/AY770624 GENBANK/AY770625 GENBANK/AY770626 GENBANK/AY772214 GENBANK/AY772215 GENBANK/AY772216 GENBANK/AY772217 GENBANK/AY772218 GENBANK/AY772219 GENBANK/AY772220 GENBANK/AY772221 GENBANK/AY779742 GENBANK/AY779743 GENBANK/AY779744 GENBANK/AY779745 GENBANK/AY779746 GENBANK/AY779747 GENBANK/AY779748 GENBANK/AY779749 GENBANK/AY780795 GENBANK/AY780796 GENBANK/AY780797 GENBANK/AY780798 GENBANK/AY780799 GENBANK/AY780800 GENBANK/AY780801 GENBANK/AY780802 GENBANK/AY780803 GENBANK/AY781780 GENBANK/AY781781 GENBANK/AY781782 GENBANK/AY785378 GENBANK/AY785379 GENBANK/AY785380 GENBANK/AY785381 GENBANK/AY785382 GENBANK/AY786572 GENBANK/AY786573 GENBANK/AY786574 GENBANK/AY796061 GENBANK/AY796062 GENBANK/AY796063 GENBANK/AY796064 GENBANK/AY800341 GENBANK/AY800342 GENBANK/AY800343 GENBANK/AY800344 GENBANK/AY800345 GENBANK/AY800346 GENBANK/AY800347 GENBANK/AY816337 GENBANK/AY816338 GENBANK/AY816339 GENBANK/AY816340 GENBANK/AY816341 GENBANK/AY816342 GENBANK/AY816343 GENBANK/AY816344 GENBANK/AY816345 GENBANK/AY816346 GENBANK/AY816347 GENBANK/AY816348 GENBANK/AY816349 GENBANK/AY816350 GENBANK/AY973559 GENBANK/AY973560 GENBANK/AY973561 GENBANK/AY973562 GENBANK/AY973563 GENBANK/AY973564 GENBANK/AY973565 GENBANK/AY973566 GENBANK/AY973567 GENBANK/AY973568 GENBANK/AY973569 GENBANK/AY973570 GENBANK/AY973571 GENBANK/AY973572 GENBANK/AY973573 GENBANK/AY973574 GENBANK/AY973575 GENBANK/AY974058 GENBANK/AY974059 GENBANK/AY974060 GENBANK/AY974061 GENBANK/AY974062 GENBANK/AY974063 GENBANK/AY974064 GENBANK/AY974065 GENBANK/AY974066 GENBANK/AY974067 GENBANK/AY974068 GENBANK/AY974069 GENBANK/DQ004899 GENBANK/DQ004900 GENBANK/DQ004901 GENBANK/DQ004902 GENBANK/DQ004903 GENBANK/DQ004904 GENBANK/DQ004905 GENBANK/DQ004906 GENBANK/DQ004907 GENBANK/DQ004908 GENBANK/DQ004909 GENBANK/DQ004910 GENBANK/DQ004911 GENBANK/DQ004912 GENBANK/DQ004913 GENBANK/DQ004914 GENBANK/DQ004915 GENBANK/DQ004916 GENBANK/DQ004917 GENBANK/DQ004918 GENBANK/DQ004919 GENBANK/DQ004920 GENBANK/DQ004921 GENBANK/DQ004922 GENBANK/DQ004923 GENBANK/DQ004924 GENBANK/DQ004925 GENBANK/DQ004926 GENBANK/DQ004927 GENBANK/DQ004928 GENBANK/DQ004929 GENBANK/DQ004930 GENBANK/DQ004931 GENBANK/DQ004932 GENBANK/DQ004933 GENBANK/DQ004934 GENBANK/DQ004935 GENBANK/DQ004936 GENBANK/DQ004937 GENBANK/DQ004938 GENBANK/DQ004939 GENBANK/DQ004940 GENBANK/DQ004941 GENBANK/DQ004942 GENBANK/DQ004943 GENBANK/DQ004944 Animals Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal Spacer/*analysis Humans Molecular Sequence Data Mollicutes/*classification/genetics Oligonucleotide Array Sequence Analysis/*methods Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sensitivity and Specificity Sequence Analysis, DNA/*methods Species Specificity
Remarks: We have completed sequencing the 16S-23S rRNA intergenic transcribed spacer (ITS) region of most known Mycoplasma , Acholeplasma , Ureaplasma , Mesoplasma , and Spiroplasma species. Analysis of the sequence data revealed a significant interspecies variability and low intraspecies polymorphism of the ITS region among Mollicutes . This finding enabled the application of a combined polymerase chain reaction-microarray technology for identifying Mollicutes species. The microarray included individual species-specific oligonucleotide probes for characterizing human Mollicutes species and other species known to be common cell line contaminants. Evaluation of the microarray was conducted using multiple, previously characterized, Mollicutes species. The microarray analysis of the samples used demonstrated a highly specific assay, which is capable of rapid and accurate discrimination among Mollicutes species.
URL: 16470366
Ref #: 11949
Author(s): Artiushin,S.;Duvall,M.;Minion,F.C.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of mycoplasma strain ISM1499 and its assignment to the Acholeplasma oculi strain cluster
Volume: 45
Page(s): 104-9
Year: 1995
Keyword(s): GENBANK/M23932 GENBANK/U14904 GENBANK/U14905 GENBANK/U14906 Acholeplasma/*classification/genetics Base Composition Base Sequence Molecular Sequence Data Mycoplasma/*classification/genetics Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/chemistry/genetics Sequence Homology, Nucleic Acid Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S.
Remarks: A mycoplasma strain designated ISM1499 was used to develop a mycoplasma genetic system (G. G. Mahairas and F. C. Minion, J. Bacteriol. 171:1775-1780, 1989; G. G. Mahairas, C. Jian, and F. C. Minion, Gene 93:61-65, 1990), but phenotypic inconsistencies led to the conclusion that this organism had been classified incorrectly as a member of the species Mycoplasma pulmonis. Studies were initiated to determine the proper taxonomic position of ISM1499, and on the basis of the results of our genetic analysis, this strain was assigned to the Acholeplasma oculi strain cluster. The base composition of strain ISM1499 was identical to the base composition of A. oculi 19L, but not to the base composition of Acholeplasma laidlawii PG8 (28.3 and 30.7 mol% G+C, respectively). The taxonomic position of ISM1499 was examined by performing a parsimony analysis with 16S ribosomal DNA sequence data, and the results were compared with previous phylogenetic reconstructions. Our results indicated that ISM1499 is more closely related phylogenetically to A. oculi 19L than to A. laidlawii PG8 and JA1. Heterogeneity in the 16S ribosomal DNA sequences of A. oculi 19L and ISM1499 and in the 16S ribosomal DNA sequences of A. laidlawii PG8 and JA1 may indicate that unusual dissimilarities occur in the 16S ribosomal DNA sequences of members of the genus Acholeplasma.
URL: 95161233
Ref #: 11947
Author(s): Kong,F.;James,G.;Gordon,S.;Zelynski,A.;Gilbert,G.L.
Journal: Appl Environ Microbiol
Title: Species-specific PCR for identification of common contaminant mollicutes in cell culture
Volume: 67
Page(s): 3195-200
Year: 2001
Keyword(s): GENBANK/AF294989 GENBANK/AF294990 GENBANK/AF294991 GENBANK/AF294992 GENBANK/AF294993 GENBANK/AF294994 GENBANK/AF294995 GENBANK/AF294996 Cell Line/*microbiology DNA Primers DNA, Ribosomal Spacer/genetics Human Molecular Sequence Data Mollicutes/*classification/genetics/*isolation & purification Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sensitivity and Specificity Sequence Analysis, DNA Species Specificity
Remarks: Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and Acholeplasma laidlawii are the members of the class Mollicutes most commonly found in contaminated cell cultures. Previous studies have shown that the published PCR primer pairs designed to detect mollicutes in cell cultures are not entirely specific. The 16S rRNA gene, the 16S-23S rRNA intergenic spacer region, and the 5' end of the 23S rRNA gene, as a whole, are promising targets for design of mollicute species-specific primer pairs. We analyzed the 16S rRNA genes, the 16S-23S rRNA intergenic spacer regions, and the 5' end of the 23S rRNA genes of these mollicutes and developed PCR methods for species identification based on these regions. Using high melting temperatures, we developed a rapid-cycle PCR for detection and identification of contaminant mollicutes. Previously published, putative mollicute-specific primers amplified DNA from 73 contaminated cell lines, but the presence of mollicutes was confirmed by species-specific PCR in only 60. Sequences of the remaining 13 amplicons were identified as those of gram-positive bacterial species. Species-specific PCR primers are needed to confirm the presence of mollicutes in specimens and for identification, if required.
URL: 21318714
Data: (Sewage. A, ATCC 23206) Type strain / MRL Colindale in 1967/ D.G. ff. Edward, Beckenham / Sabine, A.B. (1941) Bact. Rev. 5, 1 / Edward, D.G. ff. & Freundt, E.A. (1956) J. gen. Microbiol. 14, 197 / Edward, D.G. ff. & Freundt, E. A. (1970) J. gen. Microbiol. 62, 1
Accession Date: 01/01/1967
History: RECEIVED FROM EDWARD D G FF,BECKENHAM
Authority: (Freundt 1955) Edward and Freundt 1970
Depositor: MRL-PHLS COLINDALE
Taxonomy: TaxLink: S43 (Acholeplasma laidlawii (Freundt 1955) Edward and Freundt 1970) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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