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Bacteria Collection: Moraxella (branhamella) catarrhalis

NCTC Number: NCTC 11020
Current Name: Moraxella (branhamella) catarrhalis
Original Strain Reference: ATCC 25238
Other Collection No: ATCC 25238; N-8; NELL
Previous Catalogue Name: Branhamella catarrhalis
Type Strain: Yes
Family: Moraxellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, aerobic
Conditions for growth on liquid media: blood agar,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247838
16S rRNA Gene Sequence: >gb|X74903|NCTC 11020 T|M.catarrhalis gene for 16S rRNA.| aggcttaacacatgc... >gb|AJ247218|ATCC 25238|Moraxella catarrhalis 16S rRNA gene, partial.| catgcaagtcgaacg... >gb|AF005185|ATCC 25238T|Moraxella catarrhalis 16S ribosomal RNA gene, partial sequence.| ctggcggcaggctta... >gb|U10876|ATCC 25238|Moraxella catarrhalis ATCC 25238 16S rRNA gene.| aaaaaatgtcatggc...
23S rRNA Gene Sequence: >gb|AJ228758|ATCC 25238|Moraxella (Branhamella) catarrhalis 23S rRNA gene, partial.| gatacttgtttatga...
Extended Bibliography: showhide Show bibliography
Ref #: 15528
Author(s): Enright,M.C.;Carter,P.E.;MacLean,I.A.;McKenzie,H.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationships between some members of the genera Neisseria, Acinetobacter, Moraxella, and Kingella based on partial 16S ribosomal DNA sequence analysis
Volume: 44
Page(s): 387-91
Year: 1994
Keyword(s): GENBANK/X74893 GENBANK/X74894 GENBANK/X74895 GENBANK/X74896 GENBANK/X74897 GENBANK/X74898 GENBANK/X74899 GENBANK/X74900 GENBANK/X74901 GENBANK/X74902 GENBANK/X74903 Acinetobacter/classification/genetics Base Sequence DNA Primers/genetics DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Kingella/classification/genetics Molecular Sequence Data Moraxella/classification/genetics Neisseria/classification/genetics Neisseriaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: We obtained 16S ribosomal DNA (rDNA) sequence data for strains belonging to 11 species of Proteobacteria, including the type strains of Kingella kingae, Neisseria lactamica, Neisseria meningitidis, Moraxella lacunata subsp. lacunata, [Neisseria] ovis, Moraxella catarrhalis, Moraxella osloensis, [Moraxella] phenylpyruvica, and Acinetobacter lwoffii, as well as strains of Neisseria subflava and Acinetobacter calcoaceticus. The data in a distance matrix constructed by comparing the sequences supported the proposal that the genera Acinetobacter and Moraxella and [N.] ovis should be excluded from the family Neisseriaceae. Our results are consistent with hybridization data which suggest that these excluded taxa should be part of a new family, the Moraxellaceae. The strains that we studied can be divided into the following five groups: (i) M. lacunata subsp. lacunata, [N.] ovis, and M. catarrhalis; (ii) M. osloensis; (iii) [M.] phenylpyruvica; (iv) A. calcoaceticus and A. lwoffii; and (v) N. meningitidis, N. subflava, N. lactamica, and K. kingae. We agree with the previous proposal that [N.] ovis should be renamed Moraxella ovis, as this organism is closely related to Moraxella species and not to Neisseria species. The generically misnamed taxon [M.] phenylpyruvica belongs to the proposed family Moraxellaceae, but it is sufficiently different to warrant exclusion from the genus Moraxella. Further work needs to be done to investigate genetically similar species, such as Psychrobacter immobilis, before the true generic position of this organism can be determined. Automated 16S rDNA sequencing with the PCR allows workers to accurately determine phylogenetic relationships between groups of organisms.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 7520730
Ref #: 58671
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 9542079
Ref #: 13137
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity Support, Non-U.S. Gov't
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 98203182
Ref #: 11987
Author(s): Enright,M.C.;Carter,P.E.;MacLean,I.A.;McKenzie,H.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationships between some members of the genera Neisseria, Acinetobacter, Moraxella, and Kingella based on partial 16S ribosomal DNA sequence analysis
Volume: 44
Page(s): 387-91
Year: 1994
Keyword(s): GENBANK/X74893 GENBANK/X74894 GENBANK/X74895 GENBANK/X74896 GENBANK/X74897 GENBANK/X74898 GENBANK/X74899 GENBANK/X74900 GENBANK/X74901 GENBANK/X74902 GENBANK/X74903 Acinetobacter/classification/genetics Base Sequence Comparative Study DNA Primers/genetics DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Kingella/classification/genetics Molecular Sequence Data Moraxella/classification/genetics Neisseria/classification/genetics Neisseriaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't
Remarks: We obtained 16S ribosomal DNA (rDNA) sequence data for strains belonging to 11 species of Proteobacteria, including the type strains of Kingella kingae, Neisseria lactamica, Neisseria meningitidis, Moraxella lacunata subsp. lacunata, [Neisseria] ovis, Moraxella catarrhalis, Moraxella osloensis, [Moraxella] phenylpyruvica, and Acinetobacter lwoffii, as well as strains of Neisseria subflava and Acinetobacter calcoaceticus. The data in a distance matrix constructed by comparing the sequences supported the proposal that the genera Acinetobacter and Moraxella and [N.] ovis should be excluded from the family Neisseriaceae. Our results are consistent with hybridization data which suggest that these excluded taxa should be part of a new family, the Moraxellaceae. The strains that we studied can be divided into the following five groups: (i) M. lacunata subsp. lacunata, [N.] ovis, and M. catarrhalis; (ii) M. osloensis; (iii) [M.] phenylpyruvica; (iv) A. calcoaceticus and A. lwoffii; and (v) N. meningitidis, N. subflava, N. lactamica, and K. kingae. We agree with the previous proposal that [N.] ovis should be renamed Moraxella ovis, as this organism is closely related to Moraxella species and not to Neisseria species. The generically misnamed taxon [M.] phenylpyruvica belongs to the proposed family Moraxellaceae, but it is sufficiently different to warrant exclusion from the genus Moraxella. Further work needs to be done to investigate genetically similar species, such as Psychrobacter immobilis, before the true generic position of this organism can be determined. Automated 16S rDNA sequencing with the PCR allows workers to accurately determine phylogenetic relationships between groups of organisms.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 94347600
Data: (N-8, Ne11) Type strain / NCTC Identification in 1975 / ATCC in 1972 / B. W. Catlin
Accession Date: 01/01/1975
History: ATCC IN 1972-CATLIN B W -UNIV OF ROCHESTER
Authority: (Frosch and Kolle 1896) Bovre 1979
Depositor: NCTC
Taxonomy: TaxLink: S1915 (Branhamella catarrhalis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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