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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Legionella pneumophila

NCTC Number: NCTC 11192
Current Name: Legionella pneumophila
Original Strain Reference: Philadelphia 1
Other Collection No: ATCC 33152; CCUG 9568; CIP 103854; DMZ 7513; JCM 7571; PHILADELPHIA 1; WDCM 00107
Previous Catalogue Name: Legionella pneumophila
Type Strain: Yes
Family: Legionellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide
Isolated From: human, lung
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS579195
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|AF362986|NCTC 11192|L123|Legionella pneumophila isolate L123 16S-23S internal transcribedspacer, partial sequence; and tRNA-Ile gene, complete sequence.| catagaaaggcacag... >gb|AF362985|NCTC 11192|L123|Legionella pneumophila isolate L123 16S-23S internal transcribedspacer, partial sequence; and tRNA-Ala gene, complete sequence.| catagaaaggcacag... >gb|AF000655|PHILADELPHIA-1| ATCC33152|Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenicregion with tRNA-Ala-like sequence.| gcgrmagratcccyt... >gb|M36023|PHILADELPHIA 1|Legionella pneumophila 16S rRNA sequence.| aactnaagagtnnna... >gb|AY298787|ATCC 33152|Legionella pneumophila 16S ribosomal RNA gene, partial sequence;16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, completesequence; and 23S ribosomal RNA gene, partial sequence.| tgtacacaccgcccg...
23S rRNA Gene Sequence: >gb|AF362986|NCTC 11192|L123|Legionella pneumophila isolate L123 16S-23S internal transcribedspacer, partial sequence; and tRNA-Ile gene, complete sequence.| catagaaaggcacag... >gb|AF362985|NCTC 11192|L123|Legionella pneumophila isolate L123 16S-23S internal transcribedspacer, partial sequence; and tRNA-Ala gene, complete sequence.| catagaaaggcacag... >gb|Z30431|PHILADELPHIA-1|ATCC 33152|L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNAand 5S ribosomal RNA.| gcctccctcaagatg... >gb|AF000655|PHILADELPHIA-1| ATCC33152|Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenicregion with tRNA-Ala-like sequence.| gcgrmagratcccyt... >gb|Z24705|PHILADELPHIA-1|ATCC 33152|L.pneumophila 23S and 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|AY298787|ATCC 33152|Legionella pneumophila 16S ribosomal RNA gene, partial sequence;16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, completesequence; and 23S ribosomal RNA gene, partial sequence.| tgtacacaccgcccg... >gb|Z24705|PHILADELPHIA-1|ATCC 33152|L.pneumophila 23S and 5S rRNA genes, partial CDS's.| gcctccctcaagatg...
Extended Bibliography: showhide Show bibliography
Ref #: 46109
Author(s): Fry,N.K.;Warwick,S.;Saunders,N.A.;Embley,T.M.
Journal: J Gen Microbiol
Title: The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae
Volume: 137
Page(s): 1215-22
Year: 1991
Keyword(s): GENBANK/M36023 GENBANK/M36024 GENBANK/M36025 GENBANK/M36026 GENBANK/M36027 GENBANK/M36028 GENBANK/M36029 GENBANK/M36030 GENBANK/M36031 GENBANK/M36032 Base Sequence Legionella/*genetics Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Species Specificity
Remarks: The 16S ribosomal RNA sequences of Legionella pneumophila, L. erythra, L. hackeliae, L. spiritensis, L. longbeachae, L. bozemanii (Fluoribacter bozemanae) and L. micdadei (Tatlockia micdadei) were determined using reverse transcriptase. The sequences were compared with published sequences for Gram-negative bacteria and phylogenetic trees were constructed. The data confirm previous work which showed that the family Legionellaceae forms a monophyletic subgroup within the gamma subdivision of the Proteobacteria. The data show that all of the legionellae studied are highly related (greater than 95%) on the basis of 16S rRNA sequences and do not support the division of the family Legionellaceae into three genera.
URL: 1713950
Ref #: 95520
Author(s): Riffard,S.;Lo Presti,F.;Normand,P.;Forey,F.;Reyrolle,M.;Etienne,J.;Vandenesch,F.
Journal: Int J Syst Bacteriol
Title: Species identification of Legionella via intergenic 16S-23S ribosomal spacer PCR analysis
Volume: 48 Pt 3
Page(s): 723-30
Year: 1998
Keyword(s): GENBANK/AF000654 GENBANK/AF000655 GENBANK/AF000656 Base Sequence Legionella/*classification/genetics Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: Species identification of Legionella in routine laboratory testing is hampered by the lack of highly discriminatory phenotypic tests. Amplification polymorphism of the intergenic 16S-23S spacer regions (ISR) has been previously developed for identification of species within the Legionellaceae [Hookey, J.V., Birtles, R.J. & Saunders, N.A. (1995). J Clin Microbiol 33, 2377-2381], but it did not provide enough resolution to distinguish all members of the bluish-white autofluorescent species and the red autofluorescent group of the Legionellaceae. By choosing new primers that target regions 4 (positions 1521-1541 of Escherichia coli 16S rRNA gene) and 6 (positions 114-132 of E.coli 23S rRNA gene) within the rDNA operon close to the 16S-23S intergenic spacer, 34 profiles were determined among the 79 type and reference strains representing 42 species that were tested. Analysis of the RFLP generated after Hinfl restriction digestion of the PCR products further improved the method, allowing complete discrimination among the species and subspecies of Legionella tested. Twenty-three well-identified strains from unrelated origins belonging to seven species gave amplification patterns identical to that of their type strain. The technique was also tested on 80 field isolates that could not be unequivocally assigned to groups by phenotypic methods. Seventy-two per cent (58/80) of these isolates had a profile identical to that of a type strain, while 27% (22/80) may correspond to new taxa since their ISR-PCR profiles did not match any of the known profiles.
URL: 9734026
Ref #: 54192
Author(s): Ko,K.S.;Lee,H.K.;Park,M.Y.;Lee,K.H.;Yun,Y.J.;Woo,S.Y.;Miyamoto,H.;Kook,Y.H.
Journal: J Clin Microbiol
Title: Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species
Volume: 40
Page(s): 2653-8
Year: 2002
Keyword(s): Bacterial Proteins/genetics Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA-Directed RNA Polymerases/*genetics *Genes, Bacterial Humans Immunophilins/genetics Legionella/classification/*enzymology/*genetics/isolation & purification Membrane Proteins/genetics *Peptidylprolyl Isomerase Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Serotyping Species Specificity
Remarks: The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species.
URL: 12089300
Ref #: 54193
Author(s): Ko,K.S.;Lee,H.K.;Park,M.Y.;Park,M.S.;Lee,K.H.;Woo,S.Y.;Yun,Y.J.;Kook,Y.H.
Journal: J Bacteriol
Title: Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene (rpoB) and DotA gene (dotA) sequences
Volume: 184
Page(s): 2123-30
Year: 2002
Keyword(s): Amino Acid Sequence *Bacterial Proteins Base Sequence Carrier Proteins/*genetics DNA-Directed RNA Polymerases/*genetics Legionella pneumophila/*classification/genetics Membrane Proteins/*genetics Molecular Sequence Data Serotyping
Remarks: The population structure of Legionella pneumophila was studied by using partial RNA polymerase gene (rpoB) and DotA gene (dotA) sequences. Trees inferred from rpoB sequences showed that two subspecies of L. pneumophila, Legionella pneumophila subsp. pneumophila and Legionella pneumophila subsp. fraseri, were clearly separated genetically. In both rpoB and dotA trees, 79 Korean isolates used in this study constituted six clonal populations, four of which (designated subgroups P-I to P-IV) were identified in L. pneumophila subsp. pneumophila and two of which (designated subgroups F-I and F-II) were identified in L. pneumophila subsp. fraseri. Although the relationships among subgroups were not identical, such subgrouping was congruent between the rpoB and dotA trees. Type strains of several serogroups did not belong to any subgroup, presumably because isolates similar to these strains were not present among our local sample of the population. There was evidence that horizontal gene transfer or recombination had occurred within L. pneumophila. Contrary to the phylogeny from rpoB and the taxonomic context, subgroups P-III and P-IV of L. pneumophila subsp. pneumophila proved to be closely related to those of L. pneumophila subsp. fraseri or showed a distinct clustering in the dotA tree. It can be inferred that dotA of subgroups P-III and P-IV has been transferred horizontally from other subspecies. The diverse distribution of serogroup 1 strains through the gene trees suggests that surface antigen-coding genes that determine serogroup can be exchanged. Thus, it can be inferred that genetic recombination has been important in the evolution of L. pneumophila.
URL: 11914343
Ref #: 95456
Author(s): Perez-Luz,S.;Fernandez,J.;Rodriguez-Valera,F.;Pascual,L.;Moreno,C.;Amo,A.;Apraiz,D.;Catalan,V.
Journal: Syst Appl Microbiol
Title: Sequence diversity of the internal transcribed spacer (ITS) region of the rRNA operons among different serogroups of Legionella pneumophila isolates
Volume: 25
Page(s): 212-9
Year: 2002
Keyword(s): Base Sequence DNA, Bacterial/chemistry/*genetics DNA, Ribosomal Spacer/*genetics Electrophoresis, Gel, Pulsed-Field Legionella pneumophila/*classification/genetics/isolation & purification Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/analysis/genetics RNA, Ribosomal, 23S/analysis/genetics RNA, Transfer/genetics Ribotyping Sequence Alignment Serotyping *Variation (Genetics) rRNA Operon/*genetics
Remarks: The genus Legionella is represented by 48 species and Legionella pneumophila includes 15 serogroups. In this work, we have studied the intergenic 16S-23S spacer region (ITS) in L. pneumophila to determine the feasability of using amplification polymorphisms in this region, to establish intraspecies differences and to discriminate Legionella species. The amplification of this region, using 16S14F and 23S0R primers, and the analysis of amplicons by the analysis of fragments technique showed that all the L. pneumophila serogroups studied presented the same electrophoretic pattern. Moreover, the analysis of different Legionella species showed that the amplification polymorphisms obtained were species-specific. In order to study the sequence variability of this region, the existence in L. pneumophila of three ribosomal operons was determined by pulsed field gel eletrophoresis (PFGE). Two of the 16S-23S rRNA ITS presented a tRNA Ala and the third one a tRNA Ile. Nevertheless, the variability expected in this region of the operon was not found and the rest of the ITS contained only punctual mutations.
URL: 12353875
Ref #: 95439
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting
Volume: 140
Page(s): 111-9
Year: 1996
Keyword(s): GENBANK/Z30431 GENBANK/Z30432 GENBANK/Z30433 GENBANK/Z30434 GENBANK/Z30435 GENBANK/Z30436 GENBANK/Z30437 GENBANK/Z30438 GENBANK/Z30439 GENBANK/Z30440 GENBANK/Z30441 GENBANK/Z30442 GENBANK/Z30443 GENBANK/Z30444 GENBANK/Z30445 GENBANK/Z30446 GENBANK/Z30447 GENBANK/Z30448 GENBANK/Z30449 GENBANK/Z30450 GENBANK/Z30451 GENBANK/Z30452 GENBANK/Z30453 GENBANK/Z30454 GENBANK/Z30455 GENBANK/Z30534 GENBANK/Z30535 GENBANK/Z30536 GENBANK/Z30537 GENBANK/Z30538 etc. Base Sequence DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Humans Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system.
URL: 8764471
Ref #: 12728
Author(s): Fry,N.K.;Warwick,S.;Saunders,N.A.;Embley,T.M.
Journal: J Gen Microbiol
Title: The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae
Volume: 137 ( Pt 5)
Page(s): 1215-22
Year: 1991
Keyword(s): GENBANK/M36023 GENBANK/M36024 GENBANK/M36025 GENBANK/M36026 GENBANK/M36027 GENBANK/M36028 GENBANK/M36029 GENBANK/M36030 GENBANK/M36031 GENBANK/M36032 Base Sequence Legionella/*genetics Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Species Specificity Support, Non-U.S. Gov't
Remarks: The 16S ribosomal RNA sequences of Legionella pneumophila, L. erythra, L. hackeliae, L. spiritensis, L. longbeachae, L. bozemanii (Fluoribacter bozemanae) and L. micdadei (Tatlockia micdadei) were determined using reverse transcriptase. The sequences were compared with published sequences for Gram-negative bacteria and phylogenetic trees were constructed. The data confirm previous work which showed that the family Legionellaceae forms a monophyletic subgroup within the gamma subdivision of the Proteobacteria. The data show that all of the legionellae studied are highly related (greater than 95%) on the basis of 16S rRNA sequences and do not support the division of the family Legionellaceae into three genera.
URL: 91324862
Ref #: 13714
Author(s): Riffard,S.;Lo Presti,F.;Normand,P.;Forey,F.;Reyrolle,M.;Etienne,J.;Vandenesch,F.
Journal: Int J Syst Bacteriol
Title: Species identification of Legionella via intergenic 16S-23S ribosomal spacer PCR analysis
Volume: 48 Pt 3
Page(s): 723-30
Year: 1998
Keyword(s): GENBANK/AF000654 GENBANK/AF000655 GENBANK/AF000656 Base Sequence Legionella/*classification/genetics Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: Species identification of Legionella in routine laboratory testing is hampered by the lack of highly discriminatory phenotypic tests. Amplification polymorphism of the intergenic 16S-23S spacer regions (ISR) has been previously developed for identification of species within the Legionellaceae [Hookey, J.V., Birtles, R.J. & Saunders, N.A. (1995). J Clin Microbiol 33, 2377-2381], but it did not provide enough resolution to distinguish all members of the bluish-white autofluorescent species and the red autofluorescent group of the Legionellaceae. By choosing new primers that target regions 4 (positions 1521-1541 of Escherichia coli 16S rRNA gene) and 6 (positions 114-132 of E.coli 23S rRNA gene) within the rDNA operon close to the 16S-23S intergenic spacer, 34 profiles were determined among the 79 type and reference strains representing 42 species that were tested. Analysis of the RFLP generated after Hinfl restriction digestion of the PCR products further improved the method, allowing complete discrimination among the species and subspecies of Legionella tested. Twenty-three well-identified strains from unrelated origins belonging to seven species gave amplification patterns identical to that of their type strain. The technique was also tested on 80 field isolates that could not be unequivocally assigned to groups by phenotypic methods. Seventy-two per cent (58/80) of these isolates had a profile identical to that of a type strain, while 27% (22/80) may correspond to new taxa since their ISR-PCR profiles did not match any of the known profiles.
URL: 98404537
Ref #: 13685
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction
Volume: 140
Page(s): 111-119
Year: 1996
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Bacterial) 0 (DNA, Ribosomal) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 23S) 0 (RNA, Ribosomal, 5S) Base Sequence Comparative Study DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Human Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of
URL: 96291654
Data: (ATCC 33152) Type strain / R. E. Weaver, CDC Atlanta in 1977 / Serogroup 1
Accession Date: 01/01/1977
History: WEAVER R E, CDC ATLANTA, GEORGIA-ISOLATED BY MCDADE J E
Authority: Brenner et al. 1979 (AL)
Depositor: WEAVER R E
Taxonomy: TaxLink: S1650 (Legionella pneumophila subsp. pneumophila Brenner et al. 1979) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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