| Extended Bibliography: |
Show bibliography
| Ref #: |
46109 |
| Author(s): |
Fry,N.K.;Warwick,S.;Saunders,N.A.;Embley,T.M. |
| Journal: |
J Gen Microbiol |
| Title: |
The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae |
| Volume: |
137 |
| Page(s): |
1215-22 |
| Year: |
1991 |
| Keyword(s): |
GENBANK/M36023
GENBANK/M36024
GENBANK/M36025
GENBANK/M36026
GENBANK/M36027
GENBANK/M36028
GENBANK/M36029
GENBANK/M36030
GENBANK/M36031
GENBANK/M36032
Base Sequence
Legionella/*genetics
Molecular Sequence Data
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
Species Specificity
|
| Remarks: |
The 16S ribosomal RNA sequences of Legionella pneumophila, L. erythra, L. hackeliae, L. spiritensis, L. longbeachae, L. bozemanii (Fluoribacter bozemanae) and L. micdadei (Tatlockia micdadei) were determined using reverse transcriptase. The sequences were compared with published sequences for Gram-negative bacteria and phylogenetic trees were constructed. The data confirm previous work which showed that the family Legionellaceae forms a monophyletic subgroup within the gamma subdivision of the Proteobacteria. The data show that all of the legionellae studied are highly related (greater than 95%) on the basis of 16S rRNA sequences and do not support the division of the family Legionellaceae into three genera. |
| URL: |
1713950 |
|
| Ref #: |
95520 |
| Author(s): |
Riffard,S.;Lo Presti,F.;Normand,P.;Forey,F.;Reyrolle,M.;Etienne,J.;Vandenesch,F. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
Species identification of Legionella via intergenic 16S-23S ribosomal spacer PCR analysis |
| Volume: |
48 Pt 3 |
| Page(s): |
723-30 |
| Year: |
1998 |
| Keyword(s): |
GENBANK/AF000654
GENBANK/AF000655
GENBANK/AF000656
Base Sequence
Legionella/*classification/genetics
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
|
| Remarks: |
Species identification of Legionella in routine laboratory testing is hampered by the lack of highly discriminatory phenotypic tests. Amplification polymorphism of the intergenic 16S-23S spacer regions (ISR) has been previously developed for identification of species within the Legionellaceae [Hookey, J.V., Birtles, R.J. & Saunders, N.A. (1995). J Clin Microbiol 33, 2377-2381], but it did not provide enough resolution to distinguish all members of the bluish-white autofluorescent species and the red autofluorescent group of the Legionellaceae. By choosing new primers that target regions 4 (positions 1521-1541 of Escherichia coli 16S rRNA gene) and 6 (positions 114-132 of E.coli 23S rRNA gene) within the rDNA operon close to the 16S-23S intergenic spacer, 34 profiles were determined among the 79 type and reference strains representing 42 species that were tested. Analysis of the RFLP generated after Hinfl restriction digestion of the PCR products further improved the method, allowing complete discrimination among the species and subspecies of Legionella tested. Twenty-three well-identified strains from unrelated origins belonging to seven species gave amplification patterns identical to that of their type strain. The technique was also tested on 80 field isolates that could not be unequivocally assigned to groups by phenotypic methods. Seventy-two per cent (58/80) of these isolates had a profile identical to that of a type strain, while 27% (22/80) may correspond to new taxa since their ISR-PCR profiles did not match any of the known profiles. |
| URL: |
9734026 |
|
| Ref #: |
54192 |
| Author(s): |
Ko,K.S.;Lee,H.K.;Park,M.Y.;Lee,K.H.;Yun,Y.J.;Woo,S.Y.;Miyamoto,H.;Kook,Y.H. |
| Journal: |
J Clin Microbiol |
| Title: |
Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species |
| Volume: |
40 |
| Page(s): |
2653-8 |
| Year: |
2002 |
| Keyword(s): |
Bacterial Proteins/genetics
Bacterial Typing Techniques
Base Sequence
DNA, Bacterial/genetics
DNA-Directed RNA Polymerases/*genetics
*Genes, Bacterial
Humans
Immunophilins/genetics
Legionella/classification/*enzymology/*genetics/isolation & purification
Membrane Proteins/genetics
*Peptidylprolyl Isomerase
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/genetics
Serotyping
Species Specificity
|
| Remarks: |
The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species. |
| URL: |
12089300 |
|
| Ref #: |
54193 |
| Author(s): |
Ko,K.S.;Lee,H.K.;Park,M.Y.;Park,M.S.;Lee,K.H.;Woo,S.Y.;Yun,Y.J.;Kook,Y.H. |
| Journal: |
J Bacteriol |
| Title: |
Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene (rpoB) and DotA gene (dotA) sequences |
| Volume: |
184 |
| Page(s): |
2123-30 |
| Year: |
2002 |
| Keyword(s): |
Amino Acid Sequence
*Bacterial Proteins
Base Sequence
Carrier Proteins/*genetics
DNA-Directed RNA Polymerases/*genetics
Legionella pneumophila/*classification/genetics
Membrane Proteins/*genetics
Molecular Sequence Data
Serotyping
|
| Remarks: |
The population structure of Legionella pneumophila was studied by using partial RNA polymerase gene (rpoB) and DotA gene (dotA) sequences. Trees inferred from rpoB sequences showed that two subspecies of L. pneumophila, Legionella pneumophila subsp. pneumophila and Legionella pneumophila subsp. fraseri, were clearly separated genetically. In both rpoB and dotA trees, 79 Korean isolates used in this study constituted six clonal populations, four of which (designated subgroups P-I to P-IV) were identified in L. pneumophila subsp. pneumophila and two of which (designated subgroups F-I and F-II) were identified in L. pneumophila subsp. fraseri. Although the relationships among subgroups were not identical, such subgrouping was congruent between the rpoB and dotA trees. Type strains of several serogroups did not belong to any subgroup, presumably because isolates similar to these strains were not present among our local sample of the population. There was evidence that horizontal gene transfer or recombination had occurred within L. pneumophila. Contrary to the phylogeny from rpoB and the taxonomic context, subgroups P-III and P-IV of L. pneumophila subsp. pneumophila proved to be closely related to those of L. pneumophila subsp. fraseri or showed a distinct clustering in the dotA tree. It can be inferred that dotA of subgroups P-III and P-IV has been transferred horizontally from other subspecies. The diverse distribution of serogroup 1 strains through the gene trees suggests that surface antigen-coding genes that determine serogroup can be exchanged. Thus, it can be inferred that genetic recombination has been important in the evolution of L. pneumophila. |
| URL: |
11914343 |
|
| Ref #: |
95456 |
| Author(s): |
Perez-Luz,S.;Fernandez,J.;Rodriguez-Valera,F.;Pascual,L.;Moreno,C.;Amo,A.;Apraiz,D.;Catalan,V. |
| Journal: |
Syst Appl Microbiol |
| Title: |
Sequence diversity of the internal transcribed spacer (ITS) region of the rRNA operons among different serogroups of Legionella pneumophila isolates |
| Volume: |
25 |
| Page(s): |
212-9 |
| Year: |
2002 |
| Keyword(s): |
Base Sequence
DNA, Bacterial/chemistry/*genetics
DNA, Ribosomal Spacer/*genetics
Electrophoresis, Gel, Pulsed-Field
Legionella pneumophila/*classification/genetics/isolation & purification
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 16S/analysis/genetics
RNA, Ribosomal, 23S/analysis/genetics
RNA, Transfer/genetics
Ribotyping
Sequence Alignment
Serotyping
*Variation (Genetics)
rRNA Operon/*genetics
|
| Remarks: |
The genus Legionella is represented by 48 species and Legionella pneumophila includes 15 serogroups. In this work, we have studied the intergenic 16S-23S spacer region (ITS) in L. pneumophila to determine the feasability of using amplification polymorphisms in this region, to establish intraspecies differences and to discriminate Legionella species. The amplification of this region, using 16S14F and 23S0R primers, and the analysis of amplicons by the analysis of fragments technique showed that all the L. pneumophila serogroups studied presented the same electrophoretic pattern. Moreover, the analysis of different Legionella species showed that the amplification polymorphisms obtained were species-specific. In order to study the sequence variability of this region, the existence in L. pneumophila of three ribosomal operons was determined by pulsed field gel eletrophoresis (PFGE). Two of the 16S-23S rRNA ITS presented a tRNA Ala and the third one a tRNA Ile. Nevertheless, the variability expected in this region of the operon was not found and the rest of the ITS contained only punctual mutations. |
| URL: |
12353875 |
|
| Ref #: |
95439 |
| Author(s): |
Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A. |
| Journal: |
FEMS Microbiol Lett |
| Title: |
Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting |
| Volume: |
140 |
| Page(s): |
111-9 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/Z30431
GENBANK/Z30432
GENBANK/Z30433
GENBANK/Z30434
GENBANK/Z30435
GENBANK/Z30436
GENBANK/Z30437
GENBANK/Z30438
GENBANK/Z30439
GENBANK/Z30440
GENBANK/Z30441
GENBANK/Z30442
GENBANK/Z30443
GENBANK/Z30444
GENBANK/Z30445
GENBANK/Z30446
GENBANK/Z30447
GENBANK/Z30448
GENBANK/Z30449
GENBANK/Z30450
GENBANK/Z30451
GENBANK/Z30452
GENBANK/Z30453
GENBANK/Z30454
GENBANK/Z30455
GENBANK/Z30534
GENBANK/Z30535
GENBANK/Z30536
GENBANK/Z30537
GENBANK/Z30538
etc.
Base Sequence
DNA Primers/genetics
DNA Probes/genetics
DNA, Bacterial/*genetics/isolation & purification
DNA, Ribosomal/genetics
Humans
Legionella/classification/*genetics/isolation & purification
Legionnaires' Disease/diagnosis/microbiology
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Bacterial/genetics
RNA, Ribosomal, 23S/genetics
RNA, Ribosomal, 5S/genetics
Sequence Homology, Nucleic Acid
Species Specificity
|
| Remarks: |
Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system. |
| URL: |
8764471 |
|
| Ref #: |
12728 |
| Author(s): |
Fry,N.K.;Warwick,S.;Saunders,N.A.;Embley,T.M. |
| Journal: |
J Gen Microbiol |
| Title: |
The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae |
| Volume: |
137 ( Pt 5) |
| Page(s): |
1215-22 |
| Year: |
1991 |
| Keyword(s): |
GENBANK/M36023
GENBANK/M36024
GENBANK/M36025
GENBANK/M36026
GENBANK/M36027
GENBANK/M36028
GENBANK/M36029
GENBANK/M36030
GENBANK/M36031
GENBANK/M36032
Base Sequence
Legionella/*genetics
Molecular Sequence Data
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
Species Specificity
Support, Non-U.S. Gov't
|
| Remarks: |
The 16S ribosomal RNA sequences of Legionella pneumophila, L. erythra, L. hackeliae, L. spiritensis, L. longbeachae, L. bozemanii (Fluoribacter bozemanae) and L. micdadei (Tatlockia micdadei) were determined using reverse transcriptase. The sequences were compared with published sequences for Gram-negative bacteria and phylogenetic trees were constructed. The data confirm previous work which showed that the family Legionellaceae forms a monophyletic subgroup within the gamma subdivision of the Proteobacteria. The data show that all of the legionellae studied are highly related (greater than 95%) on the basis of 16S rRNA sequences and do not support the division of the family Legionellaceae into three genera. |
| URL: |
91324862 |
|
| Ref #: |
13714 |
| Author(s): |
Riffard,S.;Lo Presti,F.;Normand,P.;Forey,F.;Reyrolle,M.;Etienne,J.;Vandenesch,F. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
Species identification of Legionella via intergenic 16S-23S ribosomal spacer PCR analysis |
| Volume: |
48 Pt 3 |
| Page(s): |
723-30 |
| Year: |
1998 |
| Keyword(s): |
GENBANK/AF000654
GENBANK/AF000655
GENBANK/AF000656
Base Sequence
Legionella/*classification/genetics
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
|
| Remarks: |
Species identification of Legionella in routine laboratory testing is hampered by the lack of highly discriminatory phenotypic tests. Amplification polymorphism of the intergenic 16S-23S spacer regions (ISR) has been previously developed for identification of species within the Legionellaceae [Hookey, J.V., Birtles, R.J. & Saunders, N.A. (1995). J Clin Microbiol 33, 2377-2381], but it did not provide enough resolution to distinguish all members of the bluish-white autofluorescent species and the red autofluorescent group of the Legionellaceae. By choosing new primers that target regions 4 (positions 1521-1541 of Escherichia coli 16S rRNA gene) and 6 (positions 114-132 of E.coli 23S rRNA gene) within the rDNA operon close to the 16S-23S intergenic spacer, 34 profiles were determined among the 79 type and reference strains representing 42 species that were tested. Analysis of the RFLP generated after Hinfl restriction digestion of the PCR products further improved the method, allowing complete discrimination among the species and subspecies of Legionella tested. Twenty-three well-identified strains from unrelated origins belonging to seven species gave amplification patterns identical to that of their type strain. The technique was also tested on 80 field isolates that could not be unequivocally assigned to groups by phenotypic methods. Seventy-two per cent (58/80) of these isolates had a profile identical to that of a type strain, while 27% (22/80) may correspond to new taxa since their ISR-PCR profiles did not match any of the known profiles. |
| URL: |
98404537 |
|
| Ref #: |
13685 |
| Author(s): |
Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A. |
| Journal: |
FEMS Microbiol Lett |
| Title: |
Species-specific detection of Legionella using polymerase chain reaction |
| Volume: |
140 |
| Page(s): |
111-119 |
| Year: |
1996 |
| Keyword(s): |
0 (DNA Primers)
0 (DNA Probes)
0 (DNA, Bacterial)
0 (DNA, Ribosomal)
0 (RNA, Bacterial)
0 (RNA, Ribosomal, 23S)
0 (RNA, Ribosomal, 5S)
Base Sequence
Comparative Study
DNA Primers/genetics
DNA Probes/genetics
DNA, Bacterial/*genetics/isolation & purification
DNA, Ribosomal/genetics
Human
Legionella/classification/*genetics/isolation & purification
Legionnaires' Disease/diagnosis/microbiology
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Bacterial/genetics
RNA, Ribosomal, 23S/genetics
RNA, Ribosomal, 5S/genetics
Sequence Homology, Nucleic Acid
Species Specificity
|
| Remarks: |
Legionella pneumophila and some other Legionella species are capable of |
| URL: |
96291654 |
|
|
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