Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Fluoribacter dumoffii

NCTC Number:

NCTC 11370

Current Name:

Fluoribacter dumoffii

Original Strain Reference:

NY-23

Other Collection No:

ATCC 33279; NY-23

Previous Catalogue Name:

Legionella dumoffii

Type Strain:

Yes

Family:

Legionellaceae

Hazard Group (ACDP):

Release Restrictions:

Terms & Conditions of Supply of Microbial Pathogens: Safety

Conditions for growth on solid media:

Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide

Whole Genome Sequence:

http://www.ebi.ac.uk/ena/data/view/ERS1234135

16S rRNA Gene Sequence:

>gb|X73405|ATCC 33279|L.dumoffii (ATCC 33279) gene for 16S rRNA.| gattgaacgctggcg...

23S rRNA Gene Sequence:

>gb|Z24686|NY-23|ATCC 33279|L.dumoffii 23S 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|Z24686|NY-23|ATCC 33279|L.dumoffii 23S 5S rRNA genes, partial CDS's.| gcctccctcaagatg...

Extended Bibliography:

Show bibliography

Ref #:	54192
Author(s):	Ko,K.S.;Lee,H.K.;Park,M.Y.;Lee,K.H.;Yun,Y.J.;Woo,S.Y.;Miyamoto,H.;Kook,Y.H.
Journal:	J Clin Microbiol
Title:	Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species
Volume:	40
Page(s):	2653-8
Year:	2002
Keyword(s):	Bacterial Proteins/genetics Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Genes, Bacterial Humans Immunophilins/genetics Legionella/classification/enzymology/genetics/isolation & purification Membrane Proteins/genetics *Peptidylprolyl Isomerase Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Serotyping Species Specificity
Remarks:	The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species.
URL:	12089300

Data:

(ATCC 33279) Type strain / G. K. Morris, CDC Atlanta in 1980

Accession Date:

01/01/1980

History:

MORRIS G K, CDC ATLANTA, GEORGIA

Authority:

(Brenner et al. 1980) Brown et al. 1981

Depositor:

MORRIS G K

Taxonomy:

TaxLink: S1316 (Legionella dumoffii) - Date of change: 5/02/2003

Biosafety Responsibility:

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.