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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Fluoribacter dumoffii

NCTC Number: NCTC 11370
Current Name: Fluoribacter dumoffii
Original Strain Reference: NY-23
Other Collection No: ATCC 33279; NY-23
Previous Catalogue Name: Legionella dumoffii
Type Strain: Yes
Family: Legionellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1234135
16S rRNA Gene Sequence: >gb|X73405|ATCC 33279|L.dumoffii (ATCC 33279) gene for 16S rRNA.| gattgaacgctggcg...
23S rRNA Gene Sequence: >gb|Z24686|NY-23|ATCC 33279|L.dumoffii 23S 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|Z24686|NY-23|ATCC 33279|L.dumoffii 23S 5S rRNA genes, partial CDS's.| gcctccctcaagatg...
Extended Bibliography: showhide Show bibliography
Ref #: 54192
Author(s): Ko,K.S.;Lee,H.K.;Park,M.Y.;Lee,K.H.;Yun,Y.J.;Woo,S.Y.;Miyamoto,H.;Kook,Y.H.
Journal: J Clin Microbiol
Title: Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species
Volume: 40
Page(s): 2653-8
Year: 2002
Keyword(s): Bacterial Proteins/genetics Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA-Directed RNA Polymerases/*genetics *Genes, Bacterial Humans Immunophilins/genetics Legionella/classification/*enzymology/*genetics/isolation & purification Membrane Proteins/genetics *Peptidylprolyl Isomerase Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Serotyping Species Specificity
Remarks: The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species.
URL: 12089300
Data: (ATCC 33279) Type strain / G. K. Morris, CDC Atlanta in 1980
Accession Date: 01/01/1980
History: MORRIS G K, CDC ATLANTA, GEORGIA
Authority: (Brenner et al. 1980) Brown et al. 1981
Depositor: MORRIS G K
Taxonomy: TaxLink: S1316 (Legionella dumoffii) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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