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Bacteria Collection: Cronobacter sakazakii

NCTC Number: NCTC 11467
Current Name: Cronobacter sakazakii
Original Strain Reference: CL 783/77; E4562-70
Other Collection No: ATCC 29544; CL 783/77; E4562-70, WDCM 00214
Previous Catalogue Name: Enterobacter sakazakii
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, throat
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS462977
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|EF088379|ATCC 29544|Cronobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence.| attgaacgctggcgg... >gb|EF059843|E601| ATCC 29544|Enterobacter sakazakii strain E601 16S ribosomal RNA gene, partialsequence.| ctaacacatgcaagt... >gb|AY748357|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; tRNA-Glu gene, complete sequence; and 23Sribosomal RNA gene, partial sequence.| ggagtgggttgcaaa... >gb|AY748356|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequence;and 23S ribosomal RNA gene, partial sequence.| caccatgggagtggg... >gb|AY579145|NCTC 11467|Enterobacter sakazakii strain NCTC 11467 16S ribosomal RNA gene,partial sequence.| tggagagtttgatcc... >gb|AY702093|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| gcccgtcaccccatg...
23S rRNA Gene Sequence: >gb|DQ989185|ATCC 29544|Cronobacter sakazakii strain ATCC 29544 23S ribosomal RNA gene,partial sequence.| tggctacgagggcga... >gb|AY748357|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; tRNA-Glu gene, complete sequence; and 23Sribosomal RNA gene, partial sequence.| ggagtgggttgcaaa... >gb|AY748356|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequence;and 23S ribosomal RNA gene, partial sequence.| caccatgggagtggg... >gb|AY702093|ATCC 29544|Enterobacter sakazakii strain ATCC 29544 16S ribosomal RNA gene,partial sequence; 16S-23S ribosomal RNA intergenic spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| gcccgtcaccccatg...
Bibliography: FARMER J J ET AL 1980 INT J SYST BACT 30 569 584
Extended Bibliography: showhide Show bibliography
Ref #: 43258
Author(s): Liu,Y.;Cai,X.;Zhang,X.;Gao,Q.;Yang,X.;Zheng,Z.;Luo,M.;Huang,X.
Journal: J Microbiol Methods
Title: Real time PCR using TaqMan and SYBR Green for detection of Enterobacter sakazakii in infant formula
Volume: 65
Page(s): 21-31
Year: 2006
Keyword(s): Animals Base Sequence Cattle DNA, Bacterial/chemistry/genetics DNA, Ribosomal Spacer/chemistry/genetics Enterobacter sakazakii/*genetics *Food Microbiology *Infant Formula Milk/*microbiology Molecular Sequence Data Organic Chemicals/chemistry Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/chemistry/genetics RNA, Ribosomal, 23S/chemistry/genetics Sensitivity and Specificity Sequence Analysis, DNA Taq Polymerase/chemistry
Remarks: Enterobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in neonates and children. Powdered milk-based infant formulas have been associated with the E. sakazakii-related outbreaks in premature or other immunocompromised infants. In this study, we developed two real time PCR assays using TaqMan and SYBR Green to identify the pathogen after selective enrichment in mLST and BHI. The accuracy of two detections was tested by 35 strains of E. sakazakii and 88 non-E. sakazakii bacterial strains. The results showed that all of these E. sakazakii strains were positive reaction to the detections and all of the non-E. sakazakii strains were negative. The newly developed assays enable us to detect 1.1 CFU/100 g infant formula. And both of the assays can be accomplished within 2 business days. Compared to the traditional detection, the real time PCR procedures are quicker and simpler. In this study, we also developed a new method to design the primers, which can support multiple real time PCR with one pair of primers in SYBR Green detection. The detection methods are more sensitive and effective based on Two-Tm-Value of PCR.
URL: 16081174
Ref #: 43210
Author(s): Iversen,C.;Lehner,A.;Mullane,N.;Bidlas,E.;Cleenwerck,I.;Marugg,J.;Fanning,S.;Stephan,R.;Joosten,H.
Journal: BMC Evol Biol
Title: The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1
Volume: 7
Page(s): 64
Year: 2007
Keyword(s): GENBANK/AY752936 GENBANK/AY752938 GENBANK/AY752939 GENBANK/AY752941 GENBANK/AY752942 GENBANK/AY803186 GENBANK/AY803187 GENBANK/AY803189 GENBANK/AY803190 GENBANK/AY803191 GENBANK/AY803192 GENBANK/EF059819 GENBANK/EF059820 GENBANK/EF059821 GENBANK/EF059822 GENBANK/EF059823 GENBANK/EF059824 GENBANK/EF059825 GENBANK/EF059826 GENBANK/EF059827 GENBANK/EF059828 GENBANK/EF059829 GENBANK/EF059830 GENBANK/EF059831 GENBANK/EF059832 GENBANK/EF059833 GENBANK/EF059834 GENBANK/EF059835 GENBANK/EF059836 GENBANK/EF059837 GENBANK/EF059838 GENBANK/EF059839 GENBANK/EF059840 GENBANK/EF059841 GENBANK/EF059842 GENBANK/EF059843 GENBANK/EF059844 GENBANK/EF059845 GENBANK/EF059846 GENBANK/EF059847 GENBANK/EF059848 GENBANK/EF059849 GENBANK/EF059850 GENBANK/EF059851 GENBANK/EF059852 GENBANK/EF059853 GENBANK/EF059854 GENBANK/EF059855 GENBANK/EF059856 GENBANK/EF059857 GENBANK/EF059858 GENBANK/EF059859 GENBANK/EF059860 GENBANK/EF059861 GENBANK/EF059862 GENBANK/EF059863 GENBANK/EF059864 GENBANK/EF059865 GENBANK/EF059866 GENBANK/EF059867 GENBANK/EF059868 GENBANK/EF059869 GENBANK/EF059870 GENBANK/EF059871 GENBANK/EF059872 GENBANK/EF059873 GENBANK/EF059874 GENBANK/EF059875 GENBANK/EF059876 GENBANK/EF059877 GENBANK/EF059878 GENBANK/EF059879 GENBANK/EF059880 GENBANK/EF059881 GENBANK/EF059882 GENBANK/EF059883 GENBANK/EF059884 GENBANK/EF059885 GENBANK/EF059886 GENBANK/EF059887 GENBANK/EF059888 GENBANK/EF059889 GENBANK/EF059890 GENBANK/EF059891 GENBANK/EF059892 Base Sequence DNA, Bacterial/genetics Enterobacter sakazakii/*classification/genetics Enterobacteriaceae/*classification/genetics *Genes, Bacterial Molecular Sequence Data Phenotype RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Ribotyping Species Specificity
Remarks: BACKGROUND: Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species. In this study the taxonomic relationship of strains described as E. sakazakii was further investigated. RESULTS: Strains identified as E. sakazakii were divided into separate groups on the basis of f-AFLP fingerprints, ribopatterns and full-length 16S rRNA gene sequences. DNA-DNA hybridizations revealed five genomospecies. The phenotypic profiles of the genomospecies were determined and biochemical markers identified. CONCLUSION: This study clarifies the taxonomy of E. sakazakii and proposes a reclassification of these organisms.
URL: 17439656
Ref #: 43211
Author(s): Fanjat,N.;Leclercq,A.;Joosten,H.;Robichon,D.
Journal: J Clin Microbiol
Title: Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii
Volume: 45
Page(s): 2048-50
Year: 2007
Remarks: A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMerieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non-E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.
URL: 17428936
Data: (ATCC 29544) Type strain CDC Atlanta, Georgia / Throat / Farmer, J. J. et al. (1980) Int. J. syst. Bact. 30, 569-584
Accession Date: 01/01/1982
History: ISOLATED AT CDC ATLANTA
Authority: (Farmer et al. 1980) Iverson et al 2008
Depositor: HOLMES B
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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