Culture Collections

Bacteria and Mycoplasmas detail

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Bacteria Collection: Legionella rubrilucens

NCTC Number: NCTC 11987
Current Name: Legionella rubrilucens
Original Strain Reference: WA-270A-C2
Other Collection No: ATCC 35304; CCUG 29671; CIP 103848; DSM 11884; JCM 7565
Previous Catalogue Name: Legionella rubrilucens
Type Strain: Yes
Family: Legionellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1324116
23S rRNA Gene Sequence: >gb|AY883060|NCTC11987|Legionella erythra strain NCTC11987 23S ribosomal RNA gene, partialsequence; 23S-5S ribosomal RNA intergenic spacer, completesequence; and 5S ribosomal RNA gene, partial sequence.| gaagcgtagtaatgc...
Extended Bibliography: showhide Show bibliography
Ref #: 95452
Author(s): Grattard,F.;Ginevra,C.;Riffard,S.;Ros,A.;Jarraud,S.;Etienne,J.;Pozzetto,B.
Journal: Microbes Infect
Title: Analysis of the genetic diversity of Legionella by sequencing the 23S-5S ribosomal intergenic spacer region: from phylogeny to direct identification of isolates at the species level from clinical specimens
Volume: 8
Page(s): 73-83
Year: 2006
Keyword(s): DNA, Bacterial/genetics DNA, Ribosomal Spacer/*genetics Legionella/*classification/*genetics/isolation & purification Phenotype *Phylogeny RNA, Ribosomal, 23S/*genetics Species Specificity Variation (Genetics)/*genetics
Remarks: This study focuses on the interest of the hypervariable 23S-5S ribosomal intergenic spacer region (ISR) of the genus Legionella to analyze the phylogenic diversity of Legionella at the species and subspecies levels and to identify isolates directly from clinical specimens. The method, using a real-time PCR assay with a single primer pair followed by sequencing, was able to identify correctly 49 reference strains of Legionella belonging to 37 different species, including those implicated in human infections, and to clearly differentiate the three subspecies of L. pneumophila. Based on sequence similarities, the 23S-5S ISR sequences were much more variable than the rpoB and mip sequences (P<0.0001 by the Wilcoxon signed rank test). The 23S-5S ISR method was able to cluster Legionella species in accordance with phenotypic traits, such as autofluorescence or fatty acid membrane composition. Using maximum parsimony methods, the rpoB and 23S-5S ISR data sets were shown to be incongruent (P<0.001). In contrast, the 23S-5S ISR and the mip data sets were found to be congruent (P=0.313), suggesting the interest of combining these two regions to demonstrate phylogenetic links between Legionella species. This molecular assay was shown able to both detect Legionella DNA directly in respiratory specimens from patients exhibiting a Legionella infection and provide accurate identification of the bacterium at the species level in the tested specimens. These properties open a wide range of applications to the 23S-5S ISR sequencing method, from taxonomic analyses to clinical and epidemiological investigations.
URL: 16198139
Data: (ATCC 35304) Type strain / ATCC in 1987 / G. Gorman / Brenner, D. J. et al. (1985) Int. J. syst. Bact. 35, 50
Accession Date: 04/02/2003
Authority: Brenner et al. 1985
Taxonomy: TaxLink: S3731 (Legionella rubrilucens brenner et al. 1985) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 11987) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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