Culture Collections

Bacteria and Mycoplasmas detail

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Bacteria Collection: Helicobacter canis

NCTC Number: NCTC 12739
Current Name: Helicobacter canis
Other Collection No: ATCC 51401; CCUG 32756; CIP 104753
Type Strain: Yes
Family: Helicobacteraceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Isolated From: Faeces, Dog, Switzerland, 1992
16S rRNA Gene Sequence: AY631945 (from ATCC 51401)
Miscellaneous Sequence Data: >gb|AJ969178|NCTC 12739|Helicobacter canis partial gyrB gene for gyrase B, strain NCTC12739.| gggttacacggagtt...
Bibliography: Stanley, J. et al. (1993) J. gen. Microbiol. 139, 2495
Extended Bibliography: showhide Show bibliography
Ref #: 49824
Author(s): Hannula,M.;Hanninen,M.L.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic analysis of Helicobacter species based on partial gyrB gene sequences
Volume: 57
Page(s): 444-9
Year: 2007
Keyword(s): Animals DNA Gyrase/*genetics DNA, Bacterial/genetics Dogs Feces/microbiology Helicobacter/*classification/*genetics/isolation & purification Helicobacter Infections/microbiology Humans Liver/microbiology Phylogeny Polymerase Chain Reaction Stomach/microbiology
Remarks: Analysis of 16S rRNA gene sequences is one of the most common methods for investigating the phylogeny and taxonomy of bacteria. However, several studies have indicated that the 16S rRNA gene does not distinguish between certain Helicobacter species. We therefore selected for phylogenetic analysis an alternative marker, gyrB, encoding gyrase subunit B. The aim of this investigation was to examine the applicability of gyrB gene fragments (approximately 1100 bp) for the phylogenetic study of 16 Helicobacter species and a total of 33 Helicobacter strains included in this study. Based on the sequenced fragments, a phylogenetic tree was obtained that contained two distinct clusters, with gastric species forming one cluster and enterohepatic species the other. The only exception was the gastric species Helicobacter mustelae, which clustered with the enterohepatic species. The calculated similarity matrix revealed the highest interspecies similarity between Helicobacter salomonis and Helicobacter felis (89 %) and the lowest similarity between Helicobacter pullorum and H. felis (60 %). The DNA G+C content of the sequenced fragments was < or =40 mol% in enterohepatic species and >46 mol% in gastric species, excluding Helicobacter pylori and H. mustelae, with G+C contents of 34 and 42 mol%, respectively. In summary, the gyrB gene fragments provided superior resolution and reliability to the 16S rRNA gene for differentiating between closely related Helicobacter species. A further outcome of this study was achieved by designing gyrB gene-based species-specific PCR primers for the identification of Helicobacter bizzozeronii.
URL: 17329766
Accession Date: 04/02/2003
Authority: Stanley et al. 1994
Depositor: J Stanley
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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