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Bacteria Collection: Bacillus cereus

NCTC Number: NCTC 2599
Current Name: Bacillus cereus
Original Strain Reference: Gibson 971
Other Collection No: ATCC 14579; CCM 2010; DSM 31; FORD 13; GIBSON 971; LMG 6923; NCIB 9373; NCIB 9373
Previous Catalogue Name: Bacillus cereus
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS980037
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF290547|ATCC14579|Bacillus cereus strain ATCC14579 16S ribosomal RNA gene, partialsequence.| cctggctcaggatga... >gb|X94448|DSM31|B.cereus 23S rDNA and 16S-23S spacer region.| tgaagtcgtaacaag... >gb|AJ841873|DSM 31|Bacillus cereus partial 16S rRNA gene and ITS1, strain DSM 31.| cattaagttgggcac... >gb|DQ207729|CCM 2010| ATCC 14579|Bacillus cereus strain CCM 2010 16S ribosomal RNA gene, completesequence.| agagtttgatcctgg... >gb|X89896|DSM 31|B.cereus DNA for 16S and 23S rRNA and spacer region.| gaagtcgtaacaagg...
23S rRNA Gene Sequence: >gb|X94448|DSM31|B.cereus 23S rDNA and 16S-23S spacer region.| tgaagtcgtaacaag... >gb|X89896|DSM 31|B.cereus DNA for 16S and 23S rRNA and spacer region.| gaagtcgtaacaagg...
Miscellaneous Sequence Data: >gb|AB190226|ATCC 14579|Bacillus cereus gyrB gene for gyraseB, complete cds, strain:ATCC14579.| atggaacaaaagcaa...
Bibliography: LAWRENCE J S & FORD W 1916 J BACT 1 284;SMITH N R ET AL 1964 J GEN
Extended Bibliography: showhide Show bibliography
Ref #: 95513
Author(s): Sipos,R.;Szekely,A.J.;Palatinszky,M.;Revesz,S.;Marialigeti,K.;Nikolausz,M.
Journal: FEMS Microbiol Ecol
Title: Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
Volume: 60
Page(s): 341-50
Year: 2007
Remarks: In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem. The biases associated with preferential amplification of multitemplate PCR were investigated using 'universal' bacteria-specific primers, focusing on the effect of primer mismatch, annealing temperature and PCR cycle number. The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis. When a 1 : 1 genomic DNA template mixture of two strains was used, primer mismatches inherent in the 63F primer presented a serious bias, showing preferential amplification of the template containing the perfectly matching sequence. The extent of the preferential amplification showed an almost exponential relation with increasing annealing temperature from 47 to 61 degrees C. No negative effect of the various annealing temperatures was observed with the 27F primer, with no mismatches with the target sequences. The number of PCR cycles had little influence on the template-to-product ratios. As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR.
URL: 17343679
Ref #: 95499
Author(s): Ticknor,L.O.;Kolsto,A.B.;Hill,K.K.;Keim,P.;Laker,M.T.;Tonks,M.;Jackson,P.J.
Journal: Appl Environ Microbiol
Title: Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus and Bacillus thuringiensis Soil Isolates
Volume: 67
Page(s): 4863-73
Year: 2001
Keyword(s): GENBANK/AF290545 GENBANK/AF290546 GENBANK/AF290547 GENBANK/AF290548 GENBANK/AF290549 GENBANK/AF290550 GENBANK/AF290551 GENBANK/AF290552 GENBANK/AF290553 GENBANK/AF290554 GENBANK/AF290555 GENBANK/AF290556 GENBANK/AF290557 GENBANK/AF290558 GENBANK/AF290559 GENBANK/AF290560 GENBANK/AF290561 GENBANK/AF290562 Bacillus cereus/*classification/*genetics/isolation & purification Bacillus thuringiensis/*classification/*genetics/isolation & purification Bacterial Typing Techniques Base Sequence DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Fluorescence Genes, rRNA Molecular Sequence Data Norway Phylogeny *Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA *Soil Microbiology Variation (Genetics)
Remarks: We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fragments in an automated fashion. No method is free of error, and we identified the major sources so that experiments can be designed to minimize its effect. Phylogenetic analysis of the fluorescent AFLP results reveals five genetic groups in these group 1 bacilli. The ATCC reference strains were restricted to two of the genetic groups, clearly not representative of the diversity in these bacteria. Both B. anthracis strains analyzed were closely related and affiliated with a B. cereus milk isolate (ATCC 4342) and a B. cereus human pathogenic strain (periodontitis). Across the entire study, pathogenic strains, including B. anthracis, were more closely related to one another than to the environmental isolates. Eight strains representing the five distinct phylogenetic clusters were further analyzed by comparison of their 16S rRNA gene sequences to confirm the phylogenetic status of these groups. This analysis was consistent with the AFLP analysis, although of much lower resolution. The innovation of automated genotype analysis by using a replicated and statistical approach to fragment identification will allow very large sample analyses in the future.
URL: 11571195
Ref #: 95473
Author(s): Nubel,U.;Schmidt,P.M.;Reiss,E.;Bier,F.;Beyer,W.;Naumann,D.
Journal: FEMS Microbiol Lett
Title: Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA
Volume: 240
Page(s): 215-23
Year: 2004
Keyword(s): GENBANK/AJ841817 GENBANK/AJ841818 GENBANK/AJ841819 GENBANK/AJ841820 GENBANK/AJ841821 GENBANK/AJ841822 GENBANK/AJ841823 GENBANK/AJ841824 GENBANK/AJ841825 GENBANK/AJ841826 GENBANK/AJ841827 GENBANK/AJ841828 GENBANK/AJ841829 GENBANK/AJ841830 GENBANK/AJ841831 GENBANK/AJ841832 GENBANK/AJ841833 GENBANK/AJ841834 GENBANK/AJ841835 GENBANK/AJ841836 GENBANK/AJ841837 GENBANK/AJ841838 GENBANK/AJ841839 GENBANK/AJ841840 GENBANK/AJ841841 GENBANK/AJ841842 GENBANK/AJ841843 GENBANK/AJ841844 GENBANK/AJ841845 GENBANK/AJ841846 GENBANK/AJ841847 GENBANK/AJ841848 GENBANK/AJ841849 GENBANK/AJ841850 GENBANK/AJ841851 GENBANK/AJ841852 GENBANK/AJ841853 GENBANK/AJ841854 GENBANK/AJ841855 GENBANK/AJ841856 GENBANK/AJ841857 GENBANK/AJ841858 GENBANK/AJ841859 GENBANK/AJ841860 GENBANK/AJ841861 GENBANK/AJ841862 GENBANK/AJ841863 GENBANK/AJ841864 GENBANK/AJ841865 GENBANK/AJ841866 GENBANK/AJ841867 GENBANK/AJ841868 GENBANK/AJ841869 GENBANK/AJ841870 GENBANK/AJ841871 GENBANK/AJ841872 GENBANK/AJ841873 GENBANK/AJ841874 GENBANK/AJ841875 GENBANK/AJ841876 GENBANK/AJ841877 GENBANK/AJ841878 GENBANK/AJ841879 GENBANK/AJ841880 GENBANK/AJ841881 GENBANK/AJ841882 GENBANK/AJ841883 GENBANK/AJ841884 GENBANK/AJ841885 GENBANK/AJ841886 GENBANK/AJ841887 GENBANK/AJ841888 GENBANK/AJ841889 GENBANK/AJ841890 GENBANK/AJ841891 GENBANK/AJ841892 GENBANK/AJ841893 GENBANK/AJ841894 GENBANK/AJ841895 Bacillus anthracis/*classification/genetics/*isolation & purification Base Pair Mismatch DNA, Bacterial/chemistry/genetics DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Array Sequence Analysis Phylogeny RNA, Bacterial/genetics RNA, Transfer, Ile/genetics Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Remarks: We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. When 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized to fluorescently labeled PCR amplification products, one or more mismatches could be discriminated in all but one cases. Hence, hybridization events were highly specific and identification of B. anthracis was straightforward.
URL: 15522510
Ref #: 12211
Author(s): Ticknor,L.O.;Kolsto,A.B.;Hill,K.K.;Keim,P.;Laker,M.T.;Tonks,M.;Jackson,P.J.
Journal: Appl Environ Microbiol
Title: Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus and Bacillus thuringiensis Soil Isolates
Volume: 67
Page(s): 4863-73
Year: 2001
Keyword(s): GENBANK/AF290545 GENBANK/AF290546 GENBANK/AF290547 GENBANK/AF290548 GENBANK/AF290549 GENBANK/AF290550 GENBANK/AF290551 GENBANK/AF290552 GENBANK/AF290553 GENBANK/AF290554 GENBANK/AF290555 GENBANK/AF290556 GENBANK/AF290557 GENBANK/AF290558 GENBANK/AF290559 GENBANK/AF290560 GENBANK/AF290561 GENBANK/AF290562 Bacillus cereus/*classification/*genetics/isolation & purification Bacillus thuringiensis/*classification/*genetics/isolation & purification Bacterial Typing Techniques Base Sequence DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Fluorescence Genes, rRNA Molecular Sequence Data Norway Phylogeny *Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA *Soil Microbiology Support, U.S. Gov't, Non-P.H.S. Variation (Genetics)
Remarks: We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fragments in an automated fashion. No method is free of error, and we identified the major sources so that experiments can be designed to minimize its effect. Phylogenetic analysis of the fluorescent AFLP results reveals five genetic groups in these group 1 bacilli. The ATCC reference strains were restricted to two of the genetic groups, clearly not representative of the diversity in these bacteria. Both B. anthracis strains analyzed were closely related and affiliated with a B. cereus milk isolate (ATCC 4342) and a B. cereus human pathogenic strain (periodontitis). Across the entire study, pathogenic strains, including B. anthracis, were more closely related to one another than to the environmental isolates. Eight strains representing the five distinct phylogenetic clusters were further analyzed by comparison of their 16S rRNA gene sequences to confirm the phylogenetic status of these groups. This analysis was consistent with the AFLP analysis, although of much lower resolution. The innovation of automated genotype analysis by using a replicated and statistical approach to fragment identification will allow very large sample analyses in the future.
URL: 21455048
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2358
Author(s): Roberts,R.J.
Journal: Nucl. Acids Res.
Title: Restriction and modification enzymes and their recognition sequences.
Volume: 13,
Page(s): r165-r200
Year: 1985
Ref #: 2422
Author(s): White,P.J.
Journal: J. Gen. Microbiol.
Title: The nutrition of Bacillus megaterium and Bacillus cereus.
Volume: 71
Page(s): 505-514
Year: 1972
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 46
Author(s): Lawrence,J.S.;Ford,W.W.
Journal: J. Bacteriol.
Title: Studies on aerobic spore-bearing non-pathogenic bacteria. Part I.
Volume: 1
Page(s): 273-320
Year: 1916
Ref #: 6615
Author(s): Heyndrickx,M.;Vandermeulebroecke,K.;Kersters,K.;DeVos,P.;Logan,N.A.;Aziz,M.A.;Ali,N.;Berkeley,R.C.W.
Journal: Int. J. Syst. Bacteriol.
Title: A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended description of
Volume: 46
Page(s): 988-1003
Year: 1996
Data: (Ford 13, ATCC 14579, NCIB 9373, DSM 31) Type strain / T. Gibson, Edinburgh in 163 / Lawrence, J. S. & Ford, W. (1916) J. Bact. 1, 284 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1963
History: EDINBURGH SCHOOL OF AGRIC
Authority: Frankland and Frankland 1887 (AL)
Depositor: GIBSON T
Taxonomy: TaxLink: S394 (Bacillus cereus Frankland and Frankland 1887) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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