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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Propionibacterium acnes

NCTC Number: NCTC 737
Current Name: Propionibacterium acnes
Original Strain Reference: Ponsonby
Other Collection No: ATCC 6919; DSM 1897; PONSONBY
Previous Catalogue Name: Propionibacterium acnes
Type Strain: Yes
Family: Propionibacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, from severe case of facial acne
16S rRNA Gene Sequence: >gb|AB042288|ATCC6919|Propionibacterium acnes gene for 16S ribosomal RNA, ATCC6919.| agagtttgatcctgg... >gb|U02904|ATCC 6919|Propionibacterium acnes ATCC 6919 16S rRNA gene, partial sequence.| ctgttgccagcacgt... >gb|AB108459|ATCC 6919|Propionibacterium acnes DNA, 16S-23S ribosomal RNA intergenicspacer region, strain: ATCC 6919.| ctaaggagtttttgt...
23S rRNA Gene Sequence: >gb|AB108459|ATCC 6919|Propionibacterium acnes DNA, 16S-23S ribosomal RNA intergenicspacer region, strain: ATCC 6919.| ctaaggagtttttgt...
Bibliography: DOUGLAS M C & GUNTER S E 1946 J BACT 52 15
Extended Bibliography: showhide Show bibliography
Ref #: 19216
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid
Volume: 32
Page(s): 335-51
Year: 1994
Keyword(s): GENBANK/U02893 GENBANK/U02894 GENBANK/U02895 GENBANK/U02896 GENBANK/U02897 GENBANK/U02898 GENBANK/U02899 GENBANK/U02900 GENBANK/U02901 GENBANK/U02902 GENBANK/U02903 GENBANK/U02904 GENBANK/U02905 GENBANK/U02906 GENBANK/U02907 GENBANK/U02908 GENBANK/U02909 GENBANK/U02910 GENBANK/U02911 GENBANK/U02912 GENBANK/U02913 GENBANK/U02914 GENBANK/U02915 GENBANK/U02916 GENBANK/U02917 GENBANK/U02918 GENBANK/U02919 GENBANK/U02920 GENBANK/U02921 GENBANK/U02922 Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Humans Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF.
URL: 7512093
Ref #: 80893
Author(s): Kunishima,S.;Inoue,C.;Kamiya,T.;Ozawa,K.
Journal: Transfusion
Title: Presence of Propionibacterium acnes in blood components
Volume: 41
Page(s): 1126-9
Year: 2001
Keyword(s): Erythrocytes/*microbiology Humans Polymerase Chain Reaction Propionibacterium acnes/genetics/*isolation & purification Sequence Homology, Nucleic Acid Staphylococcus/isolation & purification
Remarks: BACKGROUND: Sterility testing, as part of the QC of blood components at the Japanese Red Cross Aichi Blood Center between April 1998 and March 2000, showed that 10 of 5568 tested blood components (0.18%), all of which were RBC concentrates, were contaminated with bacteria. Nine isolates were Propionibacterium acnes and one was Staphylococcus capitis. STUDY DESIGN AND METHODS: To investigate the molecular relatedness of eight available P. acnes isolates, 16S rRNA gene analysis and random amplified polymorphic DNA (RAPD) analysis were performed. RESULTS: DNA sequencing analysis of the 16S rRNA gene showed that five isolates were identified as distinct strains and that three had identical sequences. RAPD analysis in the latter three isolates showed that two exhibited indistinguishable banding patterns that differed from that of the third isolate. CONCLUSION: P. acnes was the most frequent contaminant of blood components, and six of eight isolates were molecularly unrelated. Further studies are necessary to investigate the precise mechanisms of contamination.
URL: 11552069
Ref #: 13687
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic
Volume: 32
Page(s): 335-351
Year: 1994
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Human Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were
URL: 94201356
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Ref #: 907
Author(s): Johnson,J.L.;Cummins,C.S.
Journal: J. Bacteriol.
Title: Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica.
Volume: 209
Page(s): 1047-1066
Year: 1972
Data: (ATCC 6919, DSM 1897) Type strain / F. Hayden, London in 1920 / Facial acne / Douglas, M.C. & Gunter, S.E. (1946) J. Bact. 52, 15
Accession Date: 01/01/1920
Authority: (Gilchrist 1900) Douglas and Gunter 1946 (AL)
Depositor: HAYDEN F
Taxonomy: TaxLink: S2357 (Propionibacterium acnes (Gilchrist 1900) Douglas and Gunter 1946) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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