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Bacteria Collection: Virgibacillus pantothenticus

NCTC Number: NCTC 8162
Current Name: Virgibacillus pantothenticus
Original Strain Reference: CN 3038
Other Collection No: ATCC 14576; CCM 2049,DSM 26; LMG 7129; NCIB 8775
Previous Catalogue Name: Bacillus pantothenticus
Type Strain: Yes
Family: Bacillaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
16S rRNA Gene Sequence: >gb|AF478073|ATCC 14576|Virgibacillus pantothenticus clone BPANTO-1R 16S ribosomal RNAgene, partial sequence; 16S-23S internal transcribed spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AJ012667|LMG 12370|Virgibacillus proomii 16S rRNA gene, strain LMG 12370.| gcggcgtgcctaata...
23S rRNA Gene Sequence: >gb|AF478073|ATCC 14576|Virgibacillus pantothenticus clone BPANTO-1R 16S ribosomal RNAgene, partial sequence; 16S-23S internal transcribed spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata...
Extended Bibliography: showhide Show bibliography
Ref #: 24032
Author(s): Heyndrickx,M.;Lebbe,L.;Kersters,K.;Hoste,B.;De Wachter,R.;De Vos,P.;Forsyth,G.;Logan,N.A.
Journal: Int J Syst Bacteriol
Title: Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998
Volume: 49 Pt 3
Page(s): 1083-90
Year: 1999
Keyword(s): GENBANK/AJ012667 Animals Bacillus/chemistry/*classification/cytology/physiology Bacterial Proteins/analysis Bacterial Typing Techniques DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Electrophoresis, Polyacrylamide Gel Fatty Acids/analysis Humans Molecular Sequence Data Phenotype Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Restriction Mapping
Remarks: A polyphasic study of strains originally received as Bacillus (now Virgibacillus) pantothenticus, along with strains representing species belonging to Bacillus, Halobacillus and Paenibacillus, was undertaken using amplified rDNA restriction analysis (ARDRA), fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins and routine diagnostic characters comprising 61 biochemical tests in the API system and 15 observations of vegetative cell and sporangial morphology. It revealed the presence within Virgibacillus of an as yet undescribed new species, for which the name Virgibacillus proomii is proposed; V. proomii can be distinguished from V. pantothenticus and members of Bacillus sensu stricto, and from members of Paenibacillus and other aerobic endospore-forming bacteria, by routine phenotypic tests. The type strain of Virgibacillus proomii is LMG 12370T.
URL: 10425765
Ref #: 22093
Author(s): Xu,D.;Cote,J.C.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences
Volume: 53
Page(s): 695-704
Year: 2003
Keyword(s): GENBANK/AF478062 GENBANK/AF478063 GENBANK/AF478064 GENBANK/AF478065 GENBANK/AF478066 GENBANK/AF478067 GENBANK/AF478068 GENBANK/AF478069 GENBANK/AF478070 GENBANK/AF478071 GENBANK/AF478072 GENBANK/AF478073 GENBANK/AF478074 GENBANK/AF478075 GENBANK/AF478076 GENBANK/AF478077 GENBANK/AF478078 GENBANK/AF478079 GENBANK/AF478080 GENBANK/AF478081 GENBANK/AF478082 GENBANK/AF478083 GENBANK/AF478084 GENBANK/AF478085 GENBANK/AF478086 GENBANK/AF478087 GENBANK/AF478088 GENBANK/AF478089 GENBANK/AF478090 GENBANK/AF478091 GENBANK/AF478092 GENBANK/AF478093 GENBANK/AF478094 GENBANK/AF478095 GENBANK/AF478096 GENBANK/AF478097 GENBANK/AF478098 GENBANK/AF478099 GENBANK/AF478100 GENBANK/AF478101 GENBANK/AF478102 GENBANK/AF478103 GENBANK/AF478104 GENBANK/AF478105 GENBANK/AF478106 GENBANK/AF478107 GENBANK/AF478108 GENBANK/AF478109 GENBANK/AF478110 GENBANK/AF478111 3' Untranslated Regions/*genetics 5' Untranslated Regions/*genetics Bacillus/*classification/genetics DNA, Ribosomal/analysis DNA, Ribosomal Spacer/*analysis Genes, rRNA Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/genetics *Sequence Analysis, DNA Species Specificity
Remarks: The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment.
URL: 12807189
Ref #: 13628
Author(s): Heyndrickx,M.;Lebbe,L.;Kersters,K.;Hoste,B.;De Wachter,R.;De Vos,P.;Forsyth,G.;Logan,N.A.
Journal: Int J Syst Bacteriol
Title: Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998
Volume: 49 Pt 3
Page(s): 1083-90
Year: 1999
Keyword(s): GENBANK/AJ012667 Animal Bacillus/chemistry/*classification/cytology/physiology Bacterial Proteins/analysis Bacterial Typing Techniques DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Electrophoresis, Polyacrylamide Gel Fatty Acids/analysis Human Molecular Sequence Data Phenotype Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Restriction Mapping Support, Non-U.S. Gov't
Remarks: A polyphasic study of strains originally received as Bacillus (now Virgibacillus) pantothenticus, along with strains representing species belonging to Bacillus, Halobacillus and Paenibacillus, was undertaken using amplified rDNA restriction analysis (ARDRA), fatty acid methyl ester (FAME) analysis, SDS-PAGE of whole-cell proteins and routine diagnostic characters comprising 61 biochemical tests in the API system and 15 observations of vegetative cell and sporangial morphology. It revealed the presence within Virgibacillus of an as yet undescribed new species, for which the name Virgibacillus proomii is proposed; V. proomii can be distinguished from V. pantothenticus and members of Bacillus sensu stricto, and from members of Paenibacillus and other aerobic endospore-forming bacteria, by routine phenotypic tests. The type strain of Virgibacillus proomii is LMG 12370T.
URL: 99354497
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 7049
Author(s): Heyndrickx,M.;Lebbe,L.;Kersters,K.;DeVos,P.;Forsyth,G.;Logan,N.A.
Journal: Int. J. Syst. Bacteriol.
Title: Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus.
Volume: 48
Page(s): 99-106
Year: 1998
Ref #: 725
Author(s): Gordon,R.E.;Haynes,W.C.;Hor-NayPang,C.
Title: The genus Bacillus.
Year: 1973
Ref #: 7444
Author(s): Heyndrickx,M.;Lebbe,L.;Kersters,K.;Hoste,B.;DeWachter,R.;DeVos,P.;Forsyth,G.;Logan,N.A.
Journal: Int. J. Syst. Bacteriol.
Title: Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998.
Volume: 49
Page(s): 1083-1090
Year: 1999
Ref #: 79
Author(s): Proom,H.;Knight,B.C.J.G.
Journal: J. Gen. Microbiol.
Title: Bacillus pantothenticus (n.sp.).
Volume: 4
Page(s): 539-541
Year: 1950
Data: (ATCC 14576, NCIB 8775, NRS 1321) Type strain / H. Proom, Beckenham in 1950 / Soil / Proom, H. & Knight, B. C. J. G. (1950) J. gen. Microbiol. 4, 539 Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 04/02/2003
Authority: (Proom and Knight 1950) Heyndrickx et al. 1998
Taxonomy: TaxLink: S423 (Virgibacillus pantothenticus (proom and knight 1950) heyndrickx et al. 1998 emend. heyndrickx et al. 1999) - Date of change: 16/06/2007 by NCTC Up to 16/06/2007: ? (NCTC 8162) - Date of change: 04/02/2003
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