Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Salmonella enterica subsp. arizonae

NCTC Number: NCTC 8297
Current Name: Salmonella enterica subsp. arizonae
Original Strain Reference: DC 5
Other Collection No: ATCC 13314; CIP 8230; CN 4247; DC5; DSM 9386
Previous Catalogue Name: Salmonella enterica subsp. arizonae
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar 1,2:1,2,5,
Conditions for growth on solid media: Nutrient / Columbia blood agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS513135
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|AF008580|ATCC 13314T|Salmonella enterica IIIa 16S ribosomal RNA gene, partial sequence.| cctggctcagattga...
Bibliography: OPINION 26 1963 INT BULL BACT NOMENC TAXON 13 35
Extended Bibliography: showhide Show bibliography
Ref #: 95511
Author(s): Mollet,C.;Drancourt,M.;Raoult,D.
Journal: Mol Microbiol
Title: rpoB sequence analysis as a novel basis for bacterial identification
Volume: 26
Page(s): 1005-11
Year: 1998
Keyword(s): GENBANK/AF008577 GENBANK/AF008579 GENBANK/AF008581 GENBANK/AF008582 GENBANK/U77434 GENBANK/U77435 GENBANK/U77436 GENBANK/U77437 GENBANK/U77438 GENBANK/U77439 GENBANK/U77440 GENBANK/U77441 GENBANK/U77443 GENBANK/U77445 GENBANK/U77446 GENBANK/U77447 GENBANK/U77448 GENBANK/U77449 GENBANK/U77450 GENBANK/U77451 GENBANK/U77452 GENBANK/U77453 GENBANK/U78182 GENBANK/U78183 GENBANK/X13854 DNA-Directed RNA Polymerases/*genetics Databases, Factual Enterobacteriaceae/genetics Evolution, Molecular RNA, Ribosomal, 16S *Sequence Analysis, DNA
Remarks: Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (rpoB) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial rpoB for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the rpoB and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the rpoB sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of rpoB sequence comparison. Comparison of rpoB sequences from Enterobacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus Klebsiella to be polyphyletic. The trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that rpoB is a powerful identification tool, which may be useful for universal bacterial identification.
URL: 9426137
Ref #: 12921
Author(s): Mollet,C.;Drancourt,M.;Raoult,D.
Journal: Mol Microbiol
Title: rpoB sequence analysis as a novel basis for bacterial identification
Volume: 26
Page(s): 1005-11
Year: 1998
Keyword(s): GENBANK/AF008577 GENBANK/AF008579 GENBANK/AF008581 GENBANK/AF008582 GENBANK/U77434 GENBANK/U77435 GENBANK/U77436 GENBANK/U77437 GENBANK/U77438 GENBANK/U77439 GENBANK/U77440 GENBANK/U77441 GENBANK/U77443 GENBANK/U77445 GENBANK/U77446 GENBANK/U77447 GENBANK/U77448 GENBANK/U77449 GENBANK/U77450 GENBANK/U77451 GENBANK/U77452 GENBANK/U77453 GENBANK/U78182 GENBANK/U78183 GENBANK/X13854 DNA-Directed RNA Polymerases/*genetics Databases, Factual Enterobacteriaceae/genetics Evolution, Molecular RNA, Ribosomal, 16S *Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (rpoB) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial rpoB for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the rpoB and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the rpoB sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of rpoB sequence comparison. Comparison of rpoB sequences from Enterobacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus Klebsiella to be polyphyletic. The trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that rpoB is a powerful identification tool, which may be useful for universal bacterial identification.
URL: 98086106
Ref #: 2885
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 18.
Volume: 35
Page(s): 375-376
Year: 1985
Ref #: 5167
Author(s): LeMinor,L.;Veron,M.;Popoff,M.
Journal: Ann. Microbiol. (Inst. Pasteur)
Title: Proposition pour une nomenclature des Salmonella.
Volume: 133
Page(s): 245-254
Year: 1982
Data: (CIP 82. 30, ATCC 13314, CN 4247) Type strain of Salmonella enterica subsp. arizonae and Salmonella arizonae / P. R. Edwards, CDC Atlanta in 1953 / 1, 2:1, 2, 5 Opinion 26 (1963) Int. Bull. bact. Nomencl. Taxon. 13, 35 / Le Minor, L. et al. (1982) Ann. Microbiol. 133B, 245 / Validation List No. 18 (1985) Int. J. syst. Bact. 35, 375 / Le Minor, L. and Popoff, M. Y. (1987) Int. J. syst. Bact. 37, 465
Accession Date: 01/01/1953
History: FROM EDWARDS P R,C D C ATLANTA
Authority: (BORMAN 1957) LE MINOR and POPOFF 1987
Depositor: EDWARDS P R
Taxonomy: TaxLink: S9099 (Salmonella enterica subspecies arizonae (borman 1957) le minor and popoff 1987) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: S2665 (Salmonella arizonae arizonae) - Date of change: 5/02/2003
Other: 51:z4, z23:-Salmonella SUBSPECIES IIIa
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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