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Bacteria Collection: Actinobacillus equuli

NCTC Number: NCTC 8529
Current Name: Actinobacillus equuli
Original Strain Reference: PM 30/53 (D108)
Other Collection No: ATCC 19392; D108; PM 30/53
Previous Catalogue Name: Actinobacillus equuli
Other Names: BACILLUS VISCOSUM-EQUI
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, blood of foal which died ofsleepy-foal
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1049098
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF381186|ATCC 19392|Actinobacillus equuli 16S ribosomal RNA gene, partial sequence.| attgaacgctggcgg...
Extended Bibliography: showhide Show bibliography
Ref #: 15711
Author(s): Berthoud,H.;Frey,J.;Kuhnert,P.
Journal: Vet Microbiol
Title: Characterization of Aqx and its operon: the hemolytic RTX determinant of Actinobacillus equuli
Volume: 87
Page(s): 159-74
Year: 2002
Keyword(s): Actinobacillus/chemistry/*genetics Actinobacillus Infections/microbiology/*veterinary Animals Bacterial Toxins/chemistry/*genetics Base Sequence Blotting, Western/veterinary Cloning, Molecular DNA Probes/chemistry DNA, Bacterial/chemistry/genetics Horse Diseases/microbiology Horses Molecular Sequence Data Neutralization Tests/veterinary Operon/genetics Phylogeny Polymerase Chain Reaction/veterinary Recombinant Proteins Sequence Alignment Sequence Analysis, DNA
Remarks: Actinobacillus equuli, a member of the family Pasteurellaceae is the etiologic agent of a frequently lethal septicemia in neonatal foals as well as other more chronic diseases like arthritis, pleuritis, pneumonia or peritonitis. It may also be isolated from the oral cavity of healthy horses. Hemolytic isolates of A. equuli are known but so far no virulence determinants have been described for this bacterial species. By screening hemolytic A. equuli strains with specific gene probes, a hemolysin, designated Aqx (A. equuli RTX (repeats in the structural toxin)) was identified. This hemolysin was shown to be an RTX type of toxin by characterization of the aqxCABD operon. All hemolytic A. equuli isolates contained a functional aqxCABD operon and expressed the Aqx hemolysin as shown by genetic and phenotypic assays. The structural toxin AqxA is the hemolysin of A. equuli as shown by expression of recombinant aqx constructs in E. coli. Its hemolytic activity can be inhibited by specific antibodies raised against AqxA. Sequence analysis of the 16S rRNA gene (rrs) of the taxonomically diffuse group of A. equuli and related strains defined two phylogenetically distinct groups. The presence of the Aqx operon is not correlated with this phylogenetic grouping. The operon is found in both groups of A. equuli strains where it specifies the hemolytic activity and is supposedly to be a determinative virulence factor. The aqx operon was not found in closely related members of the Pasteurellaceae family. The description of the Aqx hemolysin will open new ways for studying the pathogenesis of A. equuli.
URL: 12034544
Ref #: 95477
Author(s): Korczak,B.;Christensen,H.;Emler,S.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogeny of the family Pasteurellaceae based on rpoB sequences
Volume: 54
Page(s): 1393-9
Year: 2004
Keyword(s): Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry DNA-Directed RNA Polymerases/*genetics Genes, rRNA Molecular Sequence Data Pasteurellaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology
Remarks: Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
URL: 15280320
Ref #: 13695
Author(s): Berthoud,H.;Frey,J.;Kuhnert,P.
Journal: Vet Microbiol
Title: Characterization of Aqx and its operon: the hemolytic RTX determinant of Actinobacillus equuli
Volume: 87
Page(s): 159-74
Year: 2002
Keyword(s): Actinobacillus/chemistry/*genetics Actinobacillus Infections/microbiology/*veterinary Animal Bacterial Toxins/chemistry/*genetics Base Sequence Blotting, Western/veterinary Cloning, Molecular DNA Probes/chemistry DNA, Bacterial/chemistry/genetics Horse Diseases/microbiology Horses Molecular Sequence Data Neutralization Tests/veterinary Operon/genetics Phylogeny Polymerase Chain Reaction/veterinary Recombinant Proteins Sequence Alignment Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: Actinobacillus equuli, a member of the family Pasteurellaceae is the etiologic agent of a frequently lethal septicemia in neonatal foals as well as other more chronic diseases like arthritis, pleuritis, pneumonia or peritonitis. It may also be isolated from the oral cavity of healthy horses. Hemolytic isolates of A. equuli are known but so far no virulence determinants have been described for this bacterial species. By screening hemolytic A. equuli strains with specific gene probes, a hemolysin, designated Aqx (A. equuli RTX (repeats in the structural toxin)) was identified. This hemolysin was shown to be an RTX type of toxin by characterization of the aqxCABD operon. All hemolytic A. equuli isolates contained a functional aqxCABD operon and expressed the Aqx hemolysin as shown by genetic and phenotypic assays. The structural toxin AqxA is the hemolysin of A. equuli as shown by expression of recombinant aqx constructs in E. coli. Its hemolytic activity can be inhibited by specific antibodies raised against AqxA. Sequence analysis of the 16S rRNA gene (rrs) of the taxonomically diffuse group of A. equuli and related strains defined two phylogenetically distinct groups. The presence of the Aqx operon is not correlated with this phylogenetic grouping. The operon is found in both groups of A. equuli strains where it specifies the hemolytic activity and is supposedly to be a determinative virulence factor. The aqx operon was not found in closely related members of the Pasteurellaceae family. The description of the Aqx hemolysin will open new ways for studying the pathogenesis of A. equuli.
URL: 22031201
Data: (ATCC 19392) Type strain / W. C. Miller, Newmarket in 1953 / Blood, foal / Bacterium viscosum-equi
Accession Date: 01/01/1953
History: EQUINE RESEARCH STATION,NEWMARKET
Authority: (van Straaten 1918) Haupt 1934 (AL)
Depositor: MILLER W C
Taxonomy: TaxLink: S7857 (Actinobacillus equuli subsp. equuli (van Straaten 1918) Haupt 1934) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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