NCTC Number: |
NCTC 10038
|
Current Name: |
Pseudomonas fluorescens
|
Original Strain Reference: |
28/5
|
Other Collection No: |
ATCC 13525; CCB 546; DSM 50090; ICPB 3200; NCIB 9046; RH 818; WDCM 00115; 28/5
|
Previous Catalogue Name: |
Pseudomonas fluorescens
|
Type Strain: |
Yes
|
Family: |
Pseudomonadaceae
|
Hazard Group (ACDP): |
2
|
Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
Conditions for growth on solid media: |
Nutrient agar, 24-48 hours, 30°C, aerobic
|
Conditions for growth on liquid media: |
nutrient broth,30, aerobic
|
Isolated From: |
freshwater;pre-filter tanks ,reading water works
|
Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS956175
|
Annotated Genome: |
ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
|
16S rRNA Gene Sequence: |
>gb|AJ308308|ICMP 3512| ATCC 13525| NCIB 9046|Pseudomonas fluorescens partial 16S rRNA gene, isolate ICMP 3512;ATCC 13525; NCIB 9046.| agtgggggataacgt... >gb|AF094725|ATCC 13525|Pseudomonas fluorescens strain ATCC 13525 16S ribosomal RNA gene,partial sequence.| tggctcagattgaac... >gb|Z76662|DSM 50090T (TYPE STRAIN)|P.fluorescens 16S rRNA gene.| attgaacgctggcgg... >gb|DQ207731|CCM 2115| ATCC13525|Pseudomonas fluorescens strain CCM 2115 16S ribosomal RNA gene,complete sequence.| agagtttgatcctgg...
|
Bibliography: |
RHODES M E 1959 J.GEN.MICROBIOL. 21 221;SUBCOMMITEE 1967 INT.J.SYST. BACT.
|
Extended Bibliography: |
Show bibliography
Ref #: |
95513 |
Author(s): |
Sipos,R.;Szekely,A.J.;Palatinszky,M.;Revesz,S.;Marialigeti,K.;Nikolausz,M. |
Journal: |
FEMS Microbiol Ecol |
Title: |
Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis |
Volume: |
60 |
Page(s): |
341-50 |
Year: |
2007 |
Remarks: |
In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem. The biases associated with preferential amplification of multitemplate PCR were investigated using 'universal' bacteria-specific primers, focusing on the effect of primer mismatch, annealing temperature and PCR cycle number. The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis. When a 1 : 1 genomic DNA template mixture of two strains was used, primer mismatches inherent in the 63F primer presented a serious bias, showing preferential amplification of the template containing the perfectly matching sequence. The extent of the preferential amplification showed an almost exponential relation with increasing annealing temperature from 47 to 61 degrees C. No negative effect of the various annealing temperatures was observed with the 27F primer, with no mismatches with the target sequences. The number of PCR cycles had little influence on the template-to-product ratios. As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR. |
URL: |
17343679 |
|
Ref #: |
95451 |
Author(s): |
Hilario,E.;Buckley,T.R.;Young,J.M. |
Journal: |
Antonie Van Leeuwenhoek |
Title: |
Improved resolution on the phylogenetic relationships among Pseudomonas by the combined analysis of atp D, car A, rec A and 16S rDNA |
Volume: |
86 |
Page(s): |
51-64 |
Year: |
2004 |
Keyword(s): |
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/*genetics
DNA, Bacterial/chemistry/isolation & purification
Evolution, Molecular
Phylogeny
Proton-Translocating ATPases/*genetics
Pseudomonas/*classification/*genetics
RNA, Ribosomal, 16S/*genetics
Rec A Recombinases/*genetics
Sequence Analysis, DNA
|
Remarks: |
A study of representatives of the bacterial genus Pseudomonas, analysing a combined data set of four molecular sequences with completely different properties and evolutionary constraints, is reported. The best evolutionary model was obtained with a hierarchical hypothesis testing program to describe each data set and the combined data set is presented and analysed under the likelihood criterion. The resolution among Pseudomonas taxa based on the combined data set analysis of the different lineages increased due to a synergistic effect of the individual data sets. The unresolved fluorescens lineage, as well as other weakly supported lineages in the single data set trees, should be revised in detail at the biochemical and molecular level. The taxonomic status of biovars of P. putida is discussed. |
URL: |
15103237 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
4303 |
Author(s): |
Kirchner,K.;Hauk,G.;Rehm,H.J. |
Journal: |
Appl. Microbiol. Biotechnol. |
Title: |
Exhaust gas purification using immobilised monocultures (biocatalysts). |
Volume: |
26 |
Page(s): |
579-587 |
Year: |
1987 |
|
Ref #: |
4602 |
Author(s): |
DeVos,P.;Kersters,K.;Gillis,M.;Segers,P.;DeLey,J. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Comamonas Davis and Park 1962 gen. nov., nom. rev. emend., and Comamonas terrigena Hugh 1962 sp. nov., nom. rev. |
Volume: |
35 |
Page(s): |
443-453 |
Year: |
1985 |
|
Ref #: |
6182 |
Author(s): |
Onken,U. |
Journal: |
Biotechnol. Bioeng. |
Title: |
Batch and continuous cultivation of Pseudomonas fluorescens at increased pressure. |
Volume: |
35 |
Page(s): |
983-989 |
Year: |
1990 |
|
Ref #: |
7293 |
Author(s): |
Yamamoto,S.;Harayama,S. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. |
Volume: |
48 |
Page(s): |
813-819 |
Year: |
1998 |
|
Ref #: |
742 |
Title: |
Bergey's manual of determinative Bacteriology, 8th ed. |
Year: |
1974 |
|
|
Data: |
(ATCC 13525, NCIB 9046, DSM 50090, RH 818, CCB 546) Type strain / M. E. Rhodes, Reading in 1959 / Pre-filter water-works tanks in 1951 / Rhodes, M. E. (1959) J. gen. Microbiol. 21, 221 / Subcommittee (1967) Int. J. syst. Bact. 17 255
|
Accession Date: |
01/01/1959
|
History: |
ISOLATED 1951,RHODES M E ,DEPT.MICROBIOL. ,READING UNIV. PRE:FR
|
Authority: |
Migula 1895 (AL)
|
Depositor: |
RHODES M E
|
Taxonomy: |
TaxLink: S2438 (Pseudomonas fluorescens Migula 1895) - Date of change: 5/02/2003
|
Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.