Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pseudomonas aeruginosa

NCTC Number: NCTC 10332
Current Name: Pseudomonas aeruginosa
Original Strain Reference: 10332
Other Collection No: ATCC 10145; CCEGB 481; DSM 50071; ICPB 2523; NCIB 8295; NRRL B-771; WDCM 00024
Previous Catalogue Name: Pseudomonas aeruginosa
Type Strain: Yes
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/SAMEA2479570
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|Z76672|DSM 50071T (TYPE STRAIN)|P.aeruginosa (strain DSM 50071T) 16S rRNA gene.| attgaacgctggcgg... >gb|AF094713|ATCC 10145|Pseudomonas aeruginosa strain ATCC 10145 16S ribosomal RNA gene,partial sequence.| gagtttgatcatggc... >gb|X06684|DSM50071|Pseudomonas aeruginosa DNA for 16S rRNA.| gaactgaagagtttg... >gb|Z76672|DSM 50071T (TYPE STRAIN)|Pseudomonas aeruginosa 16S rRNA gene, strain DSM 50071T.| attgaacgctggcgg...
23S rRNA Gene Sequence: >gb|AJ414208|ICMP 8647| ATCC 10145|Pseudomonas aeruginosa partial carA gene for carbamoylphosphatesynthetase small subunit.| cagcagatcgtcacc... >gb|Y00432|ATCC 10145|Pseudomonas aeruginosa gene for 23S ribosomal RNA.| ggtcaagtgaagaag... >gb|AY116913|ATCC 10145|Pseudomonas aeruginosa strain ATCC 10145 23S ribosomal RNA gene,partial sequence.| gaattcactagtgat... >gb|AJ549386|TYPE STRAIN: DSM 50071|Pseudomonas aeruginosa partial 23S rRNA gene, type strain DSM50071T.| cctttaaagaagcgt...
Bibliography: SUBCOMMITTEE 1970 INT.J.SYST.BACT. 20 15;LYSENKO O 1961 J.GEN. MICROBIOL
Extended Bibliography: showhide Show bibliography
Ref #: 95536
Author(s): Toschka,H.Y.;Hopfl,P.;Ludwig,W.;Schleifer,K.H.;Ulbrich,N.;Erdmann,V.A.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 16S ribosomal RNA gene from Pseudomonas aeruginosa
Volume: 16
Page(s): 2348
Year: 1988
Keyword(s): GENBANK/X06684 Base Sequence DNA, Ribosomal/*genetics Molecular Sequence Data Pseudomonas aeruginosa/*genetics RNA, Ribosomal/*genetics RNA, Ribosomal, 16S/*genetics
URL: 3128773
Ref #: 95535
Author(s): Toschka,H.Y.;Hopfl,P.;Ludwig,W.;Schleifer,K.H.;Ulbrich,N.;Erdmann,V.A.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 23S ribosomal RNA gene from Pseudomonas aeruginosa
Volume: 15
Page(s): 7182
Year: 1987
Keyword(s): GENBANK/Y00432 Base Sequence Molecular Sequence Data Operon Pseudomonas aeruginosa/*genetics RNA, Ribosomal/*genetics RNA, Ribosomal, 23S/*genetics
URL: 3116500
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 95451
Author(s): Hilario,E.;Buckley,T.R.;Young,J.M.
Journal: Antonie Van Leeuwenhoek
Title: Improved resolution on the phylogenetic relationships among Pseudomonas by the combined analysis of atp D, car A, rec A and 16S rDNA
Volume: 86
Page(s): 51-64
Year: 2004
Keyword(s): Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/*genetics DNA, Bacterial/chemistry/isolation & purification Evolution, Molecular Phylogeny Proton-Translocating ATPases/*genetics Pseudomonas/*classification/*genetics RNA, Ribosomal, 16S/*genetics Rec A Recombinases/*genetics Sequence Analysis, DNA
Remarks: A study of representatives of the bacterial genus Pseudomonas, analysing a combined data set of four molecular sequences with completely different properties and evolutionary constraints, is reported. The best evolutionary model was obtained with a hierarchical hypothesis testing program to describe each data set and the combined data set is presented and analysed under the likelihood criterion. The resolution among Pseudomonas taxa based on the combined data set analysis of the different lineages increased due to a synergistic effect of the individual data sets. The unresolved fluorescens lineage, as well as other weakly supported lineages in the single data set trees, should be revised in detail at the biochemical and molecular level. The taxonomic status of biovars of P. putida is discussed.
URL: 15103237
Ref #: 13728
Author(s): Toschka,H.Y.;Hopfl,P.;Ludwig,W.;Schleifer,K.H.;Ulbrich,N.;Erdmann,V.A.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 16S ribosomal RNA gene from Pseudomonas aeruginosa
Volume: 16
Page(s): 2348
Year: 1988
Keyword(s): GENBANK/X06684 Base Sequence DNA, Ribosomal/*genetics Molecular Sequence Data Pseudomonas aeruginosa/*genetics RNA, Ribosomal/*genetics RNA, Ribosomal, 16S/*genetics Support, Non-U.S. Gov't
URL: 88189838
Ref #: 13727
Author(s): Toschka,H.Y.;Hopfl,P.;Ludwig,W.;Schleifer,K.H.;Ulbrich,N.;Erdmann,V.A.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 23S ribosomal RNA gene from Pseudomonas aeruginosa
Volume: 15
Page(s): 7182
Year: 1987
Keyword(s): GENBANK/Y00432 Base Sequence Molecular Sequence Data Operon Pseudomonas aeruginosa/*genetics RNA, Ribosomal/*genetics RNA, Ribosomal, 23S/*genetics Support, Non-U.S. Gov't
URL: 88015558
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 5213
Author(s): Blinov,N.O.;Khoklov,A.S.;
Journal: Jena Intern. Symp. Tax.
Title: Pigments and taxonomy of actinomycetales. In: Prauser, H.(Ed.): The Actinomycetales.
Page(s): 145-154
Year: 1968
Data: (ATCC 10145, CCEB 481, NCIB 8295, RH 815) Type strain / O. Lysenko, Czechoslovakia in 1963 / Lysenko, O. (1961) J. gen. Microbiol. 25, 379 / Subcommittee (1967) Int. J. syst. Bact. 17, 255; (1970) 20, 15
Accession Date: 01/01/1963
History: FROM LYSENKO O ,LAB.OF INSECT PATHOL.,INST.OF BIOL.PRAGUE PRE:FR
Authority: (Schroeter 1872) Migula 1900 (AL)
Depositor: LYSENKO O
Taxonomy: TaxLink: S2402 (Pseudomonas aeruginosa (Schroeter 1872) Migula 1900) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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