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Bacteria Collection: Bacillus coagulans

NCTC Number: NCTC 10334
Current Name: Bacillus coagulans
Original Strain Reference: Hammer
Other Collection No: ATCC 7050; CCM 2013; DSM 1; HAMMER; N R SMITH 609; NCIB 9365; PORTER; WDCM 00002
Previous Catalogue Name: Bacillus coagulans
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|AF478062|ATCC 7050|Bacillus coagulans clone Bcoa2 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|DQ297928|ATCC 7050|Bacillus coagulans strain ATCC 7050 16S ribosomal RNA gene, partialsequence.| tggagagtttgatcc...
23S rRNA Gene Sequence: >gb|AF478062|ATCC 7050|Bacillus coagulans clone Bcoa2 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata...
Bibliography: HAMMER B W 1915 IOWA AGR EXP STA BULL 19 129; SARLES W B & HAMMER B W 1932
Extended Bibliography: showhide Show bibliography
Ref #: 22093
Author(s): Xu,D.;Cote,J.C.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences
Volume: 53
Page(s): 695-704
Year: 2003
Keyword(s): GENBANK/AF478062 GENBANK/AF478063 GENBANK/AF478064 GENBANK/AF478065 GENBANK/AF478066 GENBANK/AF478067 GENBANK/AF478068 GENBANK/AF478069 GENBANK/AF478070 GENBANK/AF478071 GENBANK/AF478072 GENBANK/AF478073 GENBANK/AF478074 GENBANK/AF478075 GENBANK/AF478076 GENBANK/AF478077 GENBANK/AF478078 GENBANK/AF478079 GENBANK/AF478080 GENBANK/AF478081 GENBANK/AF478082 GENBANK/AF478083 GENBANK/AF478084 GENBANK/AF478085 GENBANK/AF478086 GENBANK/AF478087 GENBANK/AF478088 GENBANK/AF478089 GENBANK/AF478090 GENBANK/AF478091 GENBANK/AF478092 GENBANK/AF478093 GENBANK/AF478094 GENBANK/AF478095 GENBANK/AF478096 GENBANK/AF478097 GENBANK/AF478098 GENBANK/AF478099 GENBANK/AF478100 GENBANK/AF478101 GENBANK/AF478102 GENBANK/AF478103 GENBANK/AF478104 GENBANK/AF478105 GENBANK/AF478106 GENBANK/AF478107 GENBANK/AF478108 GENBANK/AF478109 GENBANK/AF478110 GENBANK/AF478111 3' Untranslated Regions/*genetics 5' Untranslated Regions/*genetics Bacillus/*classification/genetics DNA, Ribosomal/analysis DNA, Ribosomal Spacer/*analysis Genes, rRNA Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/genetics *Sequence Analysis, DNA Species Specificity
Remarks: The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment.
URL: 12807189
Ref #: 22940
Author(s): Patel,M.A.;Ou,M.S.;Harbrucker,R.;Aldrich,H.C.;Buszko,M.L.;Ingram,L.O.;Shanmugam,K.T.
Journal: Appl Environ Microbiol
Title: Isolation and characterization of acid-tolerant, thermophilic bacteria for effective fermentation of biomass-derived sugars to lactic acid
Volume: 72
Page(s): 3228-35
Year: 2006
Keyword(s): GENBANK/DQ297925 GENBANK/DQ297926 GENBANK/DQ297927 GENBANK/DQ297928 Bacillus/*classification/genetics/*isolation & purification/metabolism *Biomass DNA, Ribosomal/analysis Fatty Acids/analysis Fermentation Glucose/*metabolism Heat Hydrogen-Ion Concentration Lactic Acid/*metabolism Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Xylose/*metabolism
Remarks: Biomass-derived sugars, such as glucose, xylose, and other minor sugars, can be readily fermented to fuel ethanol and commodity chemicals by the appropriate microbes. Due to the differences in the optimum conditions for the activity of the fungal cellulases that are required for depolymerization of cellulose to fermentable sugars and the growth and fermentation characteristics of the current industrial microbes, simultaneous saccharification and fermentation (SSF) of cellulose is envisioned at conditions that are not optimal for the fungal cellulase activity, leading to a higher-than-required cost of cellulase in SSF. We have isolated bacterial strains that grew and fermented both glucose and xylose, major components of cellulose and hemicellulose, respectively, to l(+)-lactic acid at 50 degrees C and pH 5.0, conditions that are also optimal for fungal cellulase activity. Xylose was metabolized by these new isolates through the pentose-phosphate pathway. As expected for the metabolism of xylose by the pentose-phosphate pathway, [(13)C]lactate accounted for more than 90% of the total (13)C-labeled products from [(13)C]xylose. Based on fatty acid profile and 16S rRNA sequence, these isolates cluster with Bacillus coagulans, although the B. coagulans type strain, ATCC 7050, failed to utilize xylose as a carbon source. These new B. coagulans isolates have the potential to reduce the cost of SSF by minimizing the amount of fungal cellulases, a significant cost component in the use of biomass as a renewable resource, for the production of fuels and chemicals.
URL: 16672461
Ref #: 1026
Author(s): Hammer,B.W.
Journal: Res. Bull. Iowa Agric. Exp. Stn.
Title: Bacteriological studies on the coagulation of evaporated milk.
Volume: 19
Page(s): 119-131
Year: 1915
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2277
Author(s): Frommer,W.etal.
Title: Inhibitors, obtained from bacilli, for glycoside hydrolases.
Ref #: 3394
Author(s): Blumenstock,I.
Title: Bacillus coagulans Hammer 1915 und andere thermophile oder mesophile, säuretolerante Bacillus-Arten- eine taxonomische Untersuchung. Doctoral thesis.
Year: 1984
Ref #: 3396
Author(s): Nakamura,L.K.;Blumenstock,I.;Claus,D.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic study of Bacillus coagulans Hammer 1915 with a proposal for Bacillus smithii sp. nov.
Volume: 38
Page(s): 63-73
Year: 1988
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 6519
Author(s): Watanabe,K.;Kitamura,K.;Suzuki,Y.
Journal: Appl. Environ. Microbiol.
Title: Analysis of the critical sites for protein thermostabilization by proline substitution in oligo-1,6-glucosidase from Bacillus coagulans ATCC 7050 and the evolutionary consideration of proline residues.
Volume: 62
Page(s): 2066-2073
Year: 1996
Ref #: 7652
Author(s): Wittich,R.-M.;Strömpl,C.;Moore,E.R.B.;Blasco,R.;Timmis,K.N.
Journal: J. Ind. Microbiol. Biotechnol.
Title: Interaction of Sphingomonas and Pseudomonas strains in the degradation of chlorinated dibenzofurans.
Volume: 23
Page(s): 353-358
Year: 1999
Data: (N. R. Smith 609, Porter, ATCC 7050, NCIB 9365) Type strain / T. Gibson, Edinburgh in 1963 / Hammer, B. W. (1915) Iowa Agr. Exp. Sta. Bull. 19, 129 / Sarles, W. B. & Hammer, B. W. (1932) J. Bact. 23, 301 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1963
History: GIBSON T EDINBURGH
Authority: Hammer 1915 (AL)
Depositor: GIBSON T
Taxonomy: TaxLink: S397 (Bacillus coagulans Hammer 1915) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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