| NCTC Number: |
NCTC 10341
|
| Current Name: |
Bacillus licheniformis
|
| Original Strain Reference: |
Gibson 46
|
| Other Collection No: |
ATCC 14580; CCM 2145; DSM 13; GIBSON 46; IFO 12200; NCIB 9375; WDCM 00068
|
| Previous Catalogue Name: |
Bacillus licheniformis
|
| Type Strain: |
Yes
|
| Family: |
Bacillaceae
|
| Hazard Group (ACDP): |
2
|
| Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
| Conditions for growth on solid media: |
nutrient agar,37, facultative anaerobe
|
| Conditions for growth on liquid media: |
nutrient broth,37, facultative anaerobe
|
| 16S rRNA Gene Sequence: |
>gb|AF478087|ATCC 14580|Bacillus licheniformis clone EI-3N_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23Sribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AF478086|ATCC 14580|Bacillus licheniformis clone BLICHEN-1R 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AF478085|ATCC 14580|Bacillus licheniformis clone EI-4N_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AJ289107|DSM 13 235|Rhodoferax fermentans partial 16S rRNA gene, strain DSM 13 235.| acgctggcggcatgc... >gb|AJ289106|DSM 13 234|Rhodospirillum fulvum partial 16S rRNA gene, strain DSM 13 234.| cgctggcggcaggct... >gb|AJ289105|DSM 13 233|Rhodoplanes roseus partial 16S rRNA gene, strain DSM 13 233.| agcgaacgctggcgg... >gb|X68416|DSM 13|B.licheniformis gene for 16S rRNA.| nnnnnnnagagtttg... >gb|AY940677|BNJ_PKC_38| ATCC 14580|Bacillus licheniformis strain bnj_pkc_38 16S ribosomal RNA gene,partial sequence.| tcgagcggccgacgg...
|
| 23S rRNA Gene Sequence: |
>gb|AF478087|ATCC 14580|Bacillus licheniformis clone EI-3N_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23Sribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AF478086|ATCC 14580|Bacillus licheniformis clone BLICHEN-1R 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AF478085|ATCC 14580|Bacillus licheniformis clone EI-4N_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|X68433|DSM 13|B.licheniformis gene for 23S rRNA.| ggttaagttagaaag...
|
| Extended Bibliography: |
Show bibliography
| Ref #: |
22093 |
| Author(s): |
Xu,D.;Cote,J.C. |
| Journal: |
Int J Syst Evol Microbiol |
| Title: |
Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences |
| Volume: |
53 |
| Page(s): |
695-704 |
| Year: |
2003 |
| Keyword(s): |
GENBANK/AF478062
GENBANK/AF478063
GENBANK/AF478064
GENBANK/AF478065
GENBANK/AF478066
GENBANK/AF478067
GENBANK/AF478068
GENBANK/AF478069
GENBANK/AF478070
GENBANK/AF478071
GENBANK/AF478072
GENBANK/AF478073
GENBANK/AF478074
GENBANK/AF478075
GENBANK/AF478076
GENBANK/AF478077
GENBANK/AF478078
GENBANK/AF478079
GENBANK/AF478080
GENBANK/AF478081
GENBANK/AF478082
GENBANK/AF478083
GENBANK/AF478084
GENBANK/AF478085
GENBANK/AF478086
GENBANK/AF478087
GENBANK/AF478088
GENBANK/AF478089
GENBANK/AF478090
GENBANK/AF478091
GENBANK/AF478092
GENBANK/AF478093
GENBANK/AF478094
GENBANK/AF478095
GENBANK/AF478096
GENBANK/AF478097
GENBANK/AF478098
GENBANK/AF478099
GENBANK/AF478100
GENBANK/AF478101
GENBANK/AF478102
GENBANK/AF478103
GENBANK/AF478104
GENBANK/AF478105
GENBANK/AF478106
GENBANK/AF478107
GENBANK/AF478108
GENBANK/AF478109
GENBANK/AF478110
GENBANK/AF478111
3' Untranslated Regions/*genetics
5' Untranslated Regions/*genetics
Bacillus/*classification/genetics
DNA, Ribosomal/analysis
DNA, Ribosomal Spacer/*analysis
Genes, rRNA
Molecular Sequence Data
*Phylogeny
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/genetics
*Sequence Analysis, DNA
Species Specificity
|
| Remarks: |
The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment. |
| URL: |
12807189 |
|
| Ref #: |
95468 |
| Author(s): |
Petri,R.;Imhoff,J.F. |
| Journal: |
Syst Appl Microbiol |
| Title: |
The relationship of nitrate reducing bacteria on the basis of narH gene sequences and comparison of narH and 16S rDNA based phylogeny |
| Volume: |
23 |
| Page(s): |
47-57 |
| Year: |
2000 |
| Keyword(s): |
GENBANK/AJ288121
GENBANK/AJ288122
GENBANK/AJ288123
GENBANK/AJ288124
GENBANK/AJ288125
GENBANK/AJ288126
GENBANK/AJ288127
GENBANK/AJ288128
GENBANK/AJ288129
GENBANK/AJ288130
GENBANK/AJ288131
GENBANK/AJ288132
GENBANK/AJ288133
GENBANK/AJ288134
GENBANK/AJ288135
GENBANK/AJ288136
GENBANK/AJ288137
GENBANK/AJ288138
GENBANK/AJ288139
GENBANK/AJ288140
GENBANK/AJ288141
GENBANK/AJ288142
GENBANK/AJ288143
GENBANK/AJ288144
GENBANK/AJ288145
GENBANK/AJ288146
GENBANK/AJ288147
GENBANK/AJ288148
GENBANK/AJ288149
GENBANK/AJ288150
etc.
Bacteria/classification/*genetics/metabolism
DNA, Bacterial/analysis/genetics
DNA, Ribosomal/analysis/genetics
Molecular Sequence Data
Nitrate Reductase
Nitrate Reductases/*genetics/metabolism
Nitrates/*metabolism
Oxidation-Reduction
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
Sequence Analysis, DNA
|
| Remarks: |
On the basis of available nitrate reductase gene sequences primer pairs were designed to specifically amplify gene stretches of the beta-subunit of the membrane-bound nitrate reductase (narH). Additional sequences of this gene were amplified and sequenced from pure cultures of reference species and new isolates. The distribution and phylogeny of this gene in denitrifying and nitrate-reducing bacteria was analysed. Comparison of phylogenetic trees based on 16S rDNA sequences with those based on narH sequences revealed highly similar relationships of both genes from most of the bacteria analysed. Since highly conserved functional cysteine clusters within bacterial and archaeal narH sequences support a linear evolution from one common progenitor a long evolutionary history of the respiratory membrane-bound nitrate reductase can be inferred from our phylogenetic data. |
| URL: |
10879978 |
|
| Ref #: |
13358 |
| Author(s): |
Petri,R.;Imhoff,J.F. |
| Journal: |
Syst Appl Microbiol |
| Title: |
The relationship of nitrate reducing bacteria on the basis of narH gene sequences and comparison of narH and 16S rDNA based phylogeny |
| Volume: |
23 |
| Page(s): |
47-57 |
| Year: |
2000 |
| Keyword(s): |
GENBANK/AJ288121
GENBANK/AJ288122
GENBANK/AJ288123
GENBANK/AJ288124
GENBANK/AJ288125
GENBANK/AJ288126
GENBANK/AJ288127
GENBANK/AJ288128
GENBANK/AJ288129
GENBANK/AJ288130
GENBANK/AJ288131
GENBANK/AJ288132
GENBANK/AJ288133
GENBANK/AJ288134
GENBANK/AJ288135
GENBANK/AJ288136
GENBANK/AJ288137
GENBANK/AJ288138
GENBANK/AJ288139
GENBANK/AJ288140
GENBANK/AJ288141
GENBANK/AJ288142
GENBANK/AJ288143
GENBANK/AJ288144
GENBANK/AJ288145
GENBANK/AJ288146
GENBANK/AJ288147
GENBANK/AJ288148
GENBANK/AJ288149
GENBANK/AJ288150
etc.
Bacteria/classification/*genetics/metabolism
DNA, Bacterial/analysis/genetics
DNA, Ribosomal/analysis/genetics
Molecular Sequence Data
Nitrate Reductases/*genetics/metabolism
Nitrates/*metabolism
Oxidation-Reduction
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
Sequence Analysis, DNA
Support, Non-U.S. Gov't
|
| Remarks: |
On the basis of available nitrate reductase gene sequences primer pairs were designed to specifically amplify gene stretches of the beta-subunit of the membrane-bound nitrate reductase (narH). Additional sequences of this gene were amplified and sequenced from pure cultures of reference species and new isolates. The distribution and phylogeny of this gene in denitrifying and nitrate-reducing bacteria was analysed. Comparison of phylogenetic trees based on 16S rDNA sequences with those based on narH sequences revealed highly similar relationships of both genes from most of the bacteria analysed. Since highly conserved functional cysteine clusters within bacterial and archaeal narH sequences support a linear evolution from one common progenitor a long evolutionary history of the respiratory membrane-bound nitrate reductase can be inferred from our phylogenetic data. |
| URL: |
20336325 |
|
| Ref #: |
1300 |
| Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Approved Lists of Bacterial Names. |
| Volume: |
30 |
| Page(s): |
225-420 |
| Year: |
1980 |
|
| Ref #: |
3768 |
| Author(s): |
Fahmy,F.;Flossdorf,J.;Claus,D. |
| Journal: |
System. Appl. Microbiol. |
| Title: |
The DNA base composition of the type strains of the genus Bacillus. |
| Volume: |
6 |
| Page(s): |
60-65 |
| Year: |
1985 |
|
| Ref #: |
65 |
| Author(s): |
Gibson,T. |
| Journal: |
J. Dairy Res. |
| Title: |
A study of Bacillus subtilis and related organisms. |
| Volume: |
13 |
| Page(s): |
248-260 |
| Year: |
1944 |
|
|
| Data: |
(ATCC 14580, NCIB 9375) Type strain / T. Gibson, Edinburgh in 1963 / Gibson, T (1944) J. Dairy Res. 13, 248 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
|
| Accession Date: |
01/01/1963
|
| History: |
GIBSON T EDINBURGH COLLEGE OF AGRICULTURE
|
| Authority: |
(Weigmann 1898) Chester 1901
|
| Depositor: |
GIBSON T
|
| Taxonomy: |
TaxLink: S415 (Bacillus licheniformis (Weigmann 1898) Chester 1901) - Date of change: 5/02/2003
|
| Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.
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