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Bacteria Collection: Campylobacter jejuni subsp. jejuni

NCTC Number: NCTC 11168
Current Name: Campylobacter jejuni subsp. jejuni
Original Strain Reference: 5636/77 Lucitt
Other Collection No: BIOTYPE 1; 5636/77 LUCITT; WDCM 00073
Previous Catalogue Name: Campylobacter jejuni subsp. jejuni
Type Strain: No
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar penner serotype 2
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, microaerophilic
Conditions for growth on liquid media: nutrient broth,37, microaerophilic
Isolated From: human, faeces
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS980039
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ000862|NCTC 11168|Campylobacter jejuni 16S rRNA gene, partial.| acggatctgctggaa...
23S rRNA Gene Sequence: >gb|AJ000858|NCTC 11168|Campylobacter jejuni 23S rRNA gene, partial.| tgtaagaactagtgg... >gb|X87293|BIOTYPE 1 ATCC 27562 T|V.vulnificus 23S rRNA gene, biotype 1.| ggttaagtgactaag...
Miscellaneous Sequence Data: >gb|AJ300551|CIP 5731T| ATCC 13337T| BIOTYPE 1|Hafnia alvei partial gyrB gene for DNA gyrase B subunit strain CIP5731T, ATCC 13337T, Biotype 1.| ataagtttgatgaca...
Bibliography: SKIRROW M B 1977 BR MED J II 9;BUTZLER J P ET AL 1973 J PEDIAT 82 493
Extended Bibliography: showhide Show bibliography
Ref #: 32263
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animals Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Humans Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 10206715
Ref #: 48709
Author(s): Dauga,C.
Journal: Int J Syst Evol Microbiol
Title: Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies
Volume: 52
Page(s): 531-47
Year: 2002
Keyword(s): GENBANK/AJ300528 GENBANK/AJ300529 GENBANK/AJ300530 GENBANK/AJ300531 GENBANK/AJ300532 GENBANK/AJ300533 GENBANK/AJ300534 GENBANK/AJ300535 GENBANK/AJ300536 GENBANK/AJ300537 GENBANK/AJ300538 GENBANK/AJ300539 GENBANK/AJ300540 GENBANK/AJ300541 GENBANK/AJ300542 GENBANK/AJ300543 GENBANK/AJ300544 GENBANK/AJ300545 GENBANK/AJ300546 GENBANK/AJ300547 GENBANK/AJ300548 GENBANK/AJ300549 GENBANK/AJ300550 GENBANK/AJ300551 GENBANK/AJ300552 GENBANK/AJ300553 GENBANK/AJ300554 DNA Gyrase/*genetics Enterobacteriaceae/*classification/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Phenotype RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry
Remarks: Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships.
URL: 11931166
Ref #: 12362
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animal Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Comparative Study Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Human Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 99140141
Data: M. B. Skirrow, PHLS Worcester in 1977 / Faeces / Biotype 1, Penner serotype 2 / Butzler, J. P. et al. (1973) J. Pediat. 82, 493 / Skirrow, M. B. (1977) Br. med. J. ii, 9
Accession Date: 01/01/1977
History: ISOLATED BY SKIRROW M B,WORCESTER PHLS 1977
Authority: (Jones et al. 1931) Veron and Chatelain 1973 (AL)
Depositor: SKIRROW M B
Taxonomy: TaxLink: S675 (Campylobacter jejuni subsp. jejuni (Jones et al. 1931) Veron and Chatelain 1973) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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