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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Listeria innocua

NCTC Number: NCTC 11288
Current Name: Listeria innocua
Original Strain Reference: 58/1971
Other Collection No: ATCC 33090; CCUG 15531; CIP 80.11; DSM 20649; LMG 11387; SLCC 3379; WDCM 00017; 58/1971
Previous Catalogue Name: Listeria innocua
Type Strain: Yes
Family: Listeriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar serovar six a
Conditions for growth on solid media: nutrient agar,37, facultative anaerobe
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, brain of cow after cold enrichment
16S rRNA Gene Sequence: >gb|U57915|NCTC 11288|Listeria innocua 16S and 23S ribosomal RNA genes, partial sequence,Ile-tRNA and Ala-tRNA genes, complete sequence.| agtcgtaacaaggta... >gb|U57914|NCTC 11288|Listeria innocua 16S and 23S ribosomal RNA genes, partial sequence.| agtcgtaacaaggta... >gb|X98527|ATCC33090|L.innocua DNA for 16S ribosomal RNA.| gaacgaacggaggaa... >gb|X56152|NCTC11288|L.innocua gene for 16S ribosomal RNA.| taaagagagtttgat...
23S rRNA Gene Sequence: >gb|U57915|NCTC 11288|Listeria innocua 16S and 23S ribosomal RNA genes, partial sequence,Ile-tRNA and Ala-tRNA genes, complete sequence.| agtcgtaacaaggta... >gb|U57914|NCTC 11288|Listeria innocua 16S and 23S ribosomal RNA genes, partial sequence.| agtcgtaacaaggta... >gb|X92949|ATCC 33090|L.innocua 23S rRNA gene.| atctttagtcggata...
Bibliography: SCHOOFS M DOCTORAL THESIS 1978 UNIV OF WURZBURG
Extended Bibliography: showhide Show bibliography
Ref #: 55069
Author(s): Sallen,B.;Rajoharison,A.;Desvarenne,S.;Quinn,F.;Mabilat,C.
Journal: Int J Syst Bacteriol
Title: Comparative analysis of 16S and 23S rRNA sequences of Listeria species
Volume: 46
Page(s): 669-74
Year: 1996
Keyword(s): GENBANK/X92948 GENBANK/X92949 GENBANK/X92950 GENBANK/X92951 GENBANK/X92952 GENBANK/X92953 GENBANK/X92954 Base Sequence DNA Primers DNA, Bacterial DNA, Ribosomal Listeria/classification/*genetics Molecular Sequence Data Phylogeny *RNA, Bacterial *RNA, Ribosomal, 16S *RNA, Ribosomal, 23S Sequence Homology, Nucleic Acid
Remarks: In order to establish the taxonomic value of 16S rRNA and 23S rRNA for distinguishing Listeria species, the complete 23S rRNA sequences for all Listeria species were determined by using the type strains. We designed and experimentally validated a universal 23S rRNA sequencing method, which included PCR amplification of the rDNA gene and direct cycle sequencing of the amplicon with eubacterial primers. The results of our sequence comparison indicated that the genus Listeria can be divided into two subgroups; one subgroup is composed of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri, whereas the other subgroup includes Listeria grayi subsp. grayi and Listeria grayi subsp. murrayi. A phylogenetic analysis revealed that these species diverged recently. These results are consistent with 16S rRNA sequence analysis data. For application purposes, one 16S rRNA region that can be sued to distinguish each Listeria species except L. Monocytogenes and L. innocua has been described. In this study we found four 23S rRNA signature regions which, when used in combination, can be used to distinguish the species.
URL: 8782674
Ref #: 55062
Author(s): Vaneechoutte,M.;Boerlin,P.;Tichy,H.V.;Bannerman,E.;Jager,B.;Bille,J.
Journal: Int J Syst Bacteriol
Title: Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates
Volume: 48 Pt 1
Page(s): 127-39
Year: 1998
Keyword(s): GENBANK/X98526 GENBANK/X98527 GENBANK/X98528 GENBANK/X98529 GENBANK/X98530 GENBANK/X98531 GENBANK/X98532 DNA Fingerprinting/*methods DNA, Bacterial/analysis Genes, Bacterial/genetics Listeria monocytogenes/*classification/*genetics/isolation & purification Molecular Sequence Data Phylogeny Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational RNA, Bacterial/analysis RNA, Ribosomal, 16S/analysis RNA, Transfer/analysis
Remarks: Four PCR-based DNA fingerprinting techniques were compared for their ability to identify at the species level a heterogeneous collection of isolates belonging to the six valid Listeria species. 16S rDNA-RFLP analysis identified all species and 16S rDNA-SSCP analysis identified almost all species. Also, isolates with unusual biochemical characteristics and/or unusual antigenic composition could be identified correctly. rRNA-intracistronic length polymorphism analysis suffered from high intraspecific variability, a limited number of fragments per profile, and small length differences between the spacers of different species. tRNA-intergenic length polymorphism analysis resulted in identification of all isolates but one, when fluorescent DNA capillary electrophoresis was used such that fragment length differences of 1 bp could be resolved. The four techniques yielded comparable results relevant to the taxonomy of Listeria. They all indicate a high degree of genetic relatedness between L. innocua and L. welshimeri, homogeneity of L. grayi, distinct but clear relatedness of L. grayi to the other Listeria species, a clear distinction between the two subspecies of L. ivanovii, and a clear distinction between Listeria isolates and isolates from closely related taxa or from species which are phenotypically difficult to distinguish from Listeria. New sequence determination of the 16S rRNA gene was necessary to obtain sequences in accordance with the findings of 16S rDNA-RFLP analysis.
URL: 9542083
Ref #: 55063
Author(s): Graham,T.A.;Golsteyn-Thomas,E.J.;Thomas,J.E.;Gannon,V.P.
Journal: Int J Syst Bacteriol
Title: Inter- and intraspecies comparison of the 16S-23S rRNA operon intergenic spacer regions of six Listeria spp
Volume: 47
Page(s): 863-9
Year: 1997
Keyword(s): GENBANK/U44061 GENBANK/U44062 GENBANK/U57912 GENBANK/U57913 GENBANK/U57914 GENBANK/U57915 GENBANK/U57916 GENBANK/U57917 GENBANK/U57918 GENBANK/U78980 GENBANK/U78981 GENBANK/U78982 GENBANK/U78983 GENBANK/U78984 Animals DNA, Bacterial/analysis Humans Listeria/*classification/*genetics Molecular Sequence Data Operon/genetics RNA, Ribosomal, 16S/*analysis RNA, Ribosomal, 23S/*analysis Sequence Homology, Nucleic Acid Species Specificity
Remarks: The 16S-23S rRNA intergenic spacer (IGS) regions found in six Listeria species were characterized. PCR amplification of the 16S-23S IGS with a "generic primer" set generated products of about 340 bp (small) and 550 to 590 bp (large) with DNA from all Listeria strains tested. Seven Listeria monocytogenes serotype 4b strains and one L. monocytogenes serotype 4d strain also had an additional PCR product of ca. 360 bp. The 360-bp PCR product from one of these L. monocytogenes serotype 4b strains was identical in nucleotide sequence to the small 340-bp IGS, except that it contained an 18-bp tandem repeat. The small rRNA IGSs of L. innocua, L. ivanovii, L. seeligeri, L. welshimeri, and L. grayi were 83 to 99% homologous to that of L. monocytogenes. The large rRNA IGS of L. monocytogenes was 81 to 96% homologous to those of the other Listeria species and agreed with current taxonomic division among these species. The nucleotide sequences of the central 274 bp of the large rRNA IGS of strains from seven different L. monocytogenes serotypes were highly homologous; however, serotype-specific differences were noted, and four groups were identified within L. monocytogenes based on this analysis.
URL: 9226919
Ref #: 55089
Author(s): Collins,M.D.;Wallbanks,S.;Lane,D.J.;Shah,J.;Nietupski,R.;Smida,J.;Dorsch,M.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA
Volume: 41
Page(s): 240-6
Year: 1991
Keyword(s): GENBANK/X54447 GENBANK/X56148 GENBANK/X56149 GENBANK/X56150 GENBANK/X56151 GENBANK/X56152 GENBANK/X56153 GENBANK/X56154 GENBANK/X56155 GENBANK/X56156 Base Sequence Lactobacillaceae/classification/genetics Listeria/*classification/genetics Listeria monocytogenes/classification/genetics Molecular Sequence Data *Phylogeny RNA, Bacterial/chemistry/metabolism RNA, Ribosomal, 16S/*chemistry/metabolism RNA-Directed DNA Polymerase/*metabolism
Remarks: The phylogenetic interrelationships of members of the genus Listeria were investigated by using reverse transcriptase sequencing of 16S rRNA. The sequence data indicate that at the intrageneric level the genus Listeria consists of the following two closely related but distinct lines of descent: (i) the Listeria monocytogenes group of species (including Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) and (ii) the species Listeria grayi and Listeria murrayi. At the intergeneric level a specific phylogenetic relationship between the genera Listeria and Brochothrix was evident. The sequence data clearly demonstrated that the genus Listeria is phylogenetically remote from the genus Lactobacillus and should not be included in an extended family Lactobacillaceae.
URL: 1713054
Ref #: 12314
Author(s): Collins,M.D.;Wallbanks,S.;Lane,D.J.;Shah,J.;Nietupski,R.;Smida,J.;Dorsch,M.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA
Volume: 41
Page(s): 240-6
Year: 1991
Keyword(s): GENBANK/X54447 GENBANK/X56148 GENBANK/X56149 GENBANK/X56150 GENBANK/X56151 GENBANK/X56152 GENBANK/X56153 GENBANK/X56154 GENBANK/X56155 GENBANK/X56156 Base Sequence Lactobacillaceae/classification/genetics Listeria/*classification/genetics Listeria monocytogenes/classification/genetics Molecular Sequence Data *Phylogeny RNA, Bacterial/chemistry/metabolism RNA, Ribosomal, 16S/*chemistry/metabolism RNA-Directed DNA Polymerase/*metabolism
Remarks: The phylogenetic interrelationships of members of the genus Listeria were investigated by using reverse transcriptase sequencing of 16S rRNA. The sequence data indicate that at the intrageneric level the genus Listeria consists of the following two closely related but distinct lines of descent: (i) the Listeria monocytogenes group of species (including Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) and (ii) the species Listeria grayi and Listeria murrayi. At the intergeneric level a specific phylogenetic relationship between the genera Listeria and Brochothrix was evident. The sequence data clearly demonstrated that the genus Listeria is phylogenetically remote from the genus Lactobacillus and should not be included in an extended family Lactobacillaceae.
URL: 91307972
Ref #: 13013
Author(s): Sallen,B.;Rajoharison,A.;Desvarenne,S.;Quinn,F.;Mabilat,C.
Journal: Int J Syst Bacteriol
Title: Comparative analysis of 16S and 23S rRNA sequences of Listeria species
Volume: 46
Page(s): 669-74
Year: 1996
Keyword(s): GENBANK/X92948 GENBANK/X92949 GENBANK/X92950 GENBANK/X92951 GENBANK/X92952 GENBANK/X92953 GENBANK/X92954 Base Sequence Comparative Study DNA Primers DNA, Bacterial DNA, Ribosomal Listeria/classification/*genetics Molecular Sequence Data Phylogeny *RNA, Bacterial *RNA, Ribosomal, 16S *RNA, Ribosomal, 23S Sequence Homology, Nucleic Acid
Remarks: In order to establish the taxonomic value of 16S rRNA and 23S rRNA for distinguishing Listeria species, the complete 23S rRNA sequences for all Listeria species were determined by using the type strains. We designed and experimentally validated a universal 23S rRNA sequencing method, which included PCR amplification of the rDNA gene and direct cycle sequencing of the amplicon with eubacterial primers. The results of our sequence comparison indicated that the genus Listeria can be divided into two subgroups; one subgroup is composed of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri, whereas the other subgroup includes Listeria grayi subsp. grayi and Listeria grayi subsp. murrayi. A phylogenetic analysis revealed that these species diverged recently. These results are consistent with 16S rRNA sequence analysis data. For application purposes, one 16S rRNA region that can be sued to distinguish each Listeria species except L. Monocytogenes and L. innocua has been described. In this study we found four 23S rRNA signature regions which, when used in combination, can be used to distinguish the species.
URL: 96376812
Ref #: 13012
Author(s): Vaneechoutte,M.;Boerlin,P.;Tichy,H.V.;Bannerman,E.;Jager,B.;Bille,J.
Journal: Int J Syst Bacteriol
Title: Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates
Volume: 48 Pt 1
Page(s): 127-39
Year: 1998
Keyword(s): GENBANK/X98526 GENBANK/X98527 GENBANK/X98528 GENBANK/X98529 GENBANK/X98530 GENBANK/X98531 GENBANK/X98532 Comparative Study DNA Fingerprinting/*methods DNA, Bacterial/analysis Genes, Bacterial/genetics Listeria monocytogenes/*classification/*genetics/isolation & purification Molecular Sequence Data Phylogeny Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational RNA, Bacterial/analysis RNA, Ribosomal, 16S/analysis RNA, Transfer/analysis
Remarks: Four PCR-based DNA fingerprinting techniques were compared for their ability to identify at the species level a heterogeneous collection of isolates belonging to the six valid Listeria species. 16S rDNA-RFLP analysis identified all species and 16S rDNA-SSCP analysis identified almost all species. Also, isolates with unusual biochemical characteristics and/or unusual antigenic composition could be identified correctly. rRNA-intracistronic length polymorphism analysis suffered from high intraspecific variability, a limited number of fragments per profile, and small length differences between the spacers of different species. tRNA-intergenic length polymorphism analysis resulted in identification of all isolates but one, when fluorescent DNA capillary electrophoresis was used such that fragment length differences of 1 bp could be resolved. The four techniques yielded comparable results relevant to the taxonomy of Listeria. They all indicate a high degree of genetic relatedness between L. innocua and L. welshimeri, homogeneity of L. grayi, distinct but clear relatedness of L. grayi to the other Listeria species, a clear distinction between the two subspecies of L. ivanovii, and a clear distinction between Listeria isolates and isolates from closely related taxa or from species which are phenotypically difficult to distinguish from Listeria. New sequence determination of the 16S rRNA gene was necessary to obtain sequences in accordance with the findings of 16S rDNA-RFLP analysis.
URL: 98203186
Ref #: 13011
Author(s): Graham,T.A.;Golsteyn-Thomas,E.J.;Thomas,J.E.;Gannon,V.P.
Journal: Int J Syst Bacteriol
Title: Inter- and intraspecies comparison of the 16S-23S rRNA operon intergenic spacer regions of six Listeria spp
Volume: 47
Page(s): 863-9
Year: 1997
Keyword(s): GENBANK/U44061 GENBANK/U44062 GENBANK/U57912 GENBANK/U57913 GENBANK/U57914 GENBANK/U57915 GENBANK/U57916 GENBANK/U57917 GENBANK/U57918 GENBANK/U78980 GENBANK/U78981 GENBANK/U78982 GENBANK/U78983 GENBANK/U78984 Animal Comparative Study DNA, Bacterial/analysis Human Listeria/*classification/*genetics Molecular Sequence Data Operon/genetics RNA, Ribosomal, 16S/*analysis RNA, Ribosomal, 23S/*analysis Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't
Remarks: The 16S-23S rRNA intergenic spacer (IGS) regions found in six Listeria species were characterized. PCR amplification of the 16S-23S IGS with a "generic primer" set generated products of about 340 bp (small) and 550 to 590 bp (large) with DNA from all Listeria strains tested. Seven Listeria monocytogenes serotype 4b strains and one L. monocytogenes serotype 4d strain also had an additional PCR product of ca. 360 bp. The 360-bp PCR product from one of these L. monocytogenes serotype 4b strains was identical in nucleotide sequence to the small 340-bp IGS, except that it contained an 18-bp tandem repeat. The small rRNA IGSs of L. innocua, L. ivanovii, L. seeligeri, L. welshimeri, and L. grayi were 83 to 99% homologous to that of L. monocytogenes. The large rRNA IGS of L. monocytogenes was 81 to 96% homologous to those of the other Listeria species and agreed with current taxonomic division among these species. The nucleotide sequences of the central 274 bp of the large rRNA IGS of strains from seven different L. monocytogenes serotypes were highly homologous; however, serotype-specific differences were noted, and four groups were identified within L. monocytogenes based on this analysis.
URL: 97370613
Ref #: 2494
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 10.
Volume: 33
Page(s): 438-440
Year: 1983
Ref #: 2701
Author(s): Seeliger,H.P.R.
Journal: Zentralbl. Bakteriol. Hyg. I Abt. Orig. A
Title: Apathogene Listerien: Listeria innocua sp. nov. (Seeliger and Schoofs, 1977).
Volume: 249
Page(s): 487-493
Year: 1981
Ref #: 3265
Author(s): Rocourt,J.;Grimont,F.;Grimont,P.A.D.;Seeliger,H.P.R.
Journal: Curr. Microbiol.
Title: DNA relatedness among serovars of Listeria monocytogenes (sensu lato).
Volume: 7
Page(s): 383-388
Year: 1983
Ref #: 3266
Author(s): Fiedler,F.;Seger,J.;Schrettenbrunner,A.;Seeliger,H.P.R.
Journal: System. Appl. Microbiol.
Title: The biochemistry of murein and cell wall teichoid acids in the genus Listeria.
Volume: 5
Page(s): 360-376
Year: 1984
Data: (SLCC 3379, ATCC 33090) Type strain / H. Seeliger, Wurzburg in 1979 / Brain of cow / Serovar 6a / Schoofs, M. (1978) Doctoral thesis, University of Wurzburg
Accession Date: 01/01/1979
History: ISOLATED BY WEIS 1971-SEELIGER H WURZBURG
Authority: Seeliger 1983 (ALI)
Depositor: SEELIGER H
Taxonomy: TaxLink: S1689 (Listeria innocua Seeliger 1983) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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