Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Campylobacter coli

NCTC Number: NCTC 11366
Current Name: Campylobacter coli
Original Strain Reference: 1407
Other Collection No: ATCC 33559; 1407; Brussels; CCUG 11283; CIP 7080; CIP 7080; DSM 4689; LMG 6440; WDCM 00072
Previous Catalogue Name: Campylobacter coli
Type Strain: Yes
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar penner serotype 4
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, microaerophilic
Conditions for growth on liquid media: nutrient broth,37, microaerophilic
Isolated From: mammal, pig
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS956172
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF372092|LMG 6440|Campylobacter coli strain LMG 6440 16S ribosomal RNA gene, partialsequence.| agtgaacgctggcgg...
23S rRNA Gene Sequence: >gb|X67764|LMG 6440|C.coli (LMG 6440) 23S rRNA gene.| gcgtaatagctcact...
Extended Bibliography: showhide Show bibliography
Ref #: 32263
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animals Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Humans Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 10206715
Ref #: 95450
Author(s): Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Genetic relatedness within the genus Campylobacter inferred from rpoB sequences
Volume: 56
Page(s): 937-45
Year: 2006
Keyword(s): Bacterial Proteins/*genetics Base Sequence Campylobacter/*classification/*genetics DNA Primers DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Statistics
Remarks: The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.
URL: 16627635
Ref #: 12362
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animal Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Comparative Study Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Human Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 99140141
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 4220
Author(s): Vandamme,P.;Falsen,E.;Rossau,R.;Hoste,B.;Segers,P.;Tytgat,R.;DeLey,J.
Journal: Int. J. Syst. Bacteriol.
Title: Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.
Volume: 41
Page(s): 88-103
Year: 1991
Ref #: 6325
Author(s): Véron,M.;Chatelain,R.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic study of the genus Campylobacter Sebald and Véron and designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Véron.
Volume: 23
Page(s): 122-134
Year: 1973
Data: (CIP 7080) Type strain / CIP, in 1980 / A. Florent, Brussels / Pig / Penner serotype 4
Accession Date: 01/01/1980
History: ISOLATED BY FLORENT A,BRUSSELS
Authority: (Doyle 1948) Veron and Chatelain 1973
Depositor: COLLECTION INSTITUT PASTEUR
Taxonomy: TaxLink: S667 (Campylobacter coli (Doyle 1948) Veron and Chatelain 1973) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.