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Bacteria Collection: Listeria ivanovii subsp. ivanovii

NCTC Number: NCTC 11846
Current Name: Listeria ivanovii subsp. ivanovii
Original Strain Reference: SV5 SLCC 2739
Other Collection No: ATCC 19119; SLCC 2379; SV 5; WDCM 00018
Previous Catalogue Name: Listeria ivanovii subsp. Ivanovii
Type Strain: Yes
Family: Listeriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, sheep
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1273870
16S rRNA Gene Sequence: >gb|U78981|ATCC 19119|Listeria ivanovii 16S ribosomal RNA gene, partial sequence, 16S/23Sribosomal RNA intergenic spacer region, complete sequence and 23Sribosomal RNA gene, partial sequence.| agtcgtaacaaggta... >gb|U57913|ATCC 19119|Listeria ivanovii 16S and 23S ribosomal RNA genes, partialsequence, Ile-tRNA and Ala-tRNA genes, complete sequence.| agtcgtaacaaggta... >gb|X56151|NCTC11846|L.ivanovii gene for 16S ribosomal RNA.| taaagagagtttgat...
23S rRNA Gene Sequence: >gb|U78981|ATCC 19119|Listeria ivanovii 16S ribosomal RNA gene, partial sequence, 16S/23Sribosomal RNA intergenic spacer region, complete sequence and 23Sribosomal RNA gene, partial sequence.| agtcgtaacaaggta... >gb|U57913|ATCC 19119|Listeria ivanovii 16S and 23S ribosomal RNA genes, partialsequence, Ile-tRNA and Ala-tRNA genes, complete sequence.| agtcgtaacaaggta... >gb|Y07639|ATCC 19119|L.ivanovii 23S rRNA, 5S rRNA, tRNA-Asn, tRNA-Thr, ORF Z, inlD, andinlC genes.| cgctagcatacgagg... >gb|AJ549930|TYPE STRAIN: ATCC 19119|Listeria ivanovii partial 23S rRNA gene, strain ATCC 19119.| gaacgaacggaggaa...
Bibliography: SEELIGER H P R ET AL 1984 INT J SYST 34 336
Extended Bibliography: showhide Show bibliography
Ref #: 55063
Author(s): Graham,T.A.;Golsteyn-Thomas,E.J.;Thomas,J.E.;Gannon,V.P.
Journal: Int J Syst Bacteriol
Title: Inter- and intraspecies comparison of the 16S-23S rRNA operon intergenic spacer regions of six Listeria spp
Volume: 47
Page(s): 863-9
Year: 1997
Keyword(s): GENBANK/U44061 GENBANK/U44062 GENBANK/U57912 GENBANK/U57913 GENBANK/U57914 GENBANK/U57915 GENBANK/U57916 GENBANK/U57917 GENBANK/U57918 GENBANK/U78980 GENBANK/U78981 GENBANK/U78982 GENBANK/U78983 GENBANK/U78984 Animals DNA, Bacterial/analysis Humans Listeria/*classification/*genetics Molecular Sequence Data Operon/genetics RNA, Ribosomal, 16S/*analysis RNA, Ribosomal, 23S/*analysis Sequence Homology, Nucleic Acid Species Specificity
Remarks: The 16S-23S rRNA intergenic spacer (IGS) regions found in six Listeria species were characterized. PCR amplification of the 16S-23S IGS with a "generic primer" set generated products of about 340 bp (small) and 550 to 590 bp (large) with DNA from all Listeria strains tested. Seven Listeria monocytogenes serotype 4b strains and one L. monocytogenes serotype 4d strain also had an additional PCR product of ca. 360 bp. The 360-bp PCR product from one of these L. monocytogenes serotype 4b strains was identical in nucleotide sequence to the small 340-bp IGS, except that it contained an 18-bp tandem repeat. The small rRNA IGSs of L. innocua, L. ivanovii, L. seeligeri, L. welshimeri, and L. grayi were 83 to 99% homologous to that of L. monocytogenes. The large rRNA IGS of L. monocytogenes was 81 to 96% homologous to those of the other Listeria species and agreed with current taxonomic division among these species. The nucleotide sequences of the central 274 bp of the large rRNA IGS of strains from seven different L. monocytogenes serotypes were highly homologous; however, serotype-specific differences were noted, and four groups were identified within L. monocytogenes based on this analysis.
URL: 9226919
Ref #: 55089
Author(s): Collins,M.D.;Wallbanks,S.;Lane,D.J.;Shah,J.;Nietupski,R.;Smida,J.;Dorsch,M.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA
Volume: 41
Page(s): 240-6
Year: 1991
Keyword(s): GENBANK/X54447 GENBANK/X56148 GENBANK/X56149 GENBANK/X56150 GENBANK/X56151 GENBANK/X56152 GENBANK/X56153 GENBANK/X56154 GENBANK/X56155 GENBANK/X56156 Base Sequence Lactobacillaceae/classification/genetics Listeria/*classification/genetics Listeria monocytogenes/classification/genetics Molecular Sequence Data *Phylogeny RNA, Bacterial/chemistry/metabolism RNA, Ribosomal, 16S/*chemistry/metabolism RNA-Directed DNA Polymerase/*metabolism
Remarks: The phylogenetic interrelationships of members of the genus Listeria were investigated by using reverse transcriptase sequencing of 16S rRNA. The sequence data indicate that at the intrageneric level the genus Listeria consists of the following two closely related but distinct lines of descent: (i) the Listeria monocytogenes group of species (including Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) and (ii) the species Listeria grayi and Listeria murrayi. At the intergeneric level a specific phylogenetic relationship between the genera Listeria and Brochothrix was evident. The sequence data clearly demonstrated that the genus Listeria is phylogenetically remote from the genus Lactobacillus and should not be included in an extended family Lactobacillaceae.
URL: 1713054
Ref #: 55331
Author(s): Paillard,D.;Dubois,V.;Duran,R.;Nathier,F.;Guittet,C.;Caumette,P.;Quentin,C.
Journal: Appl Environ Microbiol
Title: Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments
Volume: 69
Page(s): 6386-92
Year: 2003
Keyword(s): GENBANK/AJ535693 GENBANK/AJ535694 GENBANK/AJ535695 GENBANK/AJ535696 GENBANK/AJ535697 GENBANK/AJ535698 GENBANK/AJ535699 GENBANK/AJ535700 GENBANK/AJ535701 GENBANK/AJ549928 GENBANK/AJ549929 GENBANK/AJ549930 *Bacterial Typing Techniques Genes, rRNA Listeria/*classification/genetics Molecular Sequence Data Polymerase Chain Reaction/*methods *Polymorphism, Restriction Fragment Length RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Sewage/microbiology Time Factors Waste Disposal, Fluid/methods Water Microbiology
Remarks: A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or CfoI and, if needed, S1 was digested by either AluI or ClaI. This method was first optimized with six reference strains and then applied to 182 isolates collected from effluents of treatment plants. All isolates were also identified by the API Listeria kit, hemolysis, and phosphatidylinositol-specific phospholipase C production (PI-PLC) on ALOA medium. The PCR-RFLP method unambiguously identified 160 environmental strains, including 131 in concordance with the API system, and revealed that 22 isolates were mixed cultures of Listeria monocytogenes and Listeria innocua. Discrepant results were resolved by a multiplex PCR on the iap gene, which confirmed the PCR-RFLP data for 49 of the 51 discordances, including the 22 mixed cultures. Sequencing of the 16S rRNA gene for 12 selected strains and reconstruction of a phylogenetic tree validated the molecular methods, except for two unclassifiable strains. The 158 single identifiable isolates were 92 L. monocytogenes (including seven nonhemolytic and PI-PLC-negative strains), 61 L. innocua, 4 Listeria seeligeri, and 1 Listeria welshimeri strain. The PCR-RFLP method proposed here provides rapid, easy-to-use, inexpensive, and reliable identification of the six Listeria species. Moreover, it can detect mixtures of Listeria species and thus is particularly adapted to environmental and food microbiology.
URL: 14602590
Ref #: 55248
Author(s): Engelbrecht,F.;Dickneite,C.;Lampidis,R.;Gotz,M.;DasGupta,U.;Goebel,W.
Journal: Mol Gen Genet
Title: Sequence comparison of the chromosomal regions encompassing the internalin C genes (inlC) of Listeria monocytogenes and L. ivanovii
Volume: 257
Page(s): 186-97
Year: 1998
Keyword(s): GENBANK/Y07639 GENBANK/Y07640 Amino Acid Sequence Bacterial Proteins/*genetics/physiology Base Sequence Chromosome Mapping Chromosomes, Bacterial/*genetics DNA, Bacterial/genetics Gene Expression Regulation, Bacterial *Genes, Bacterial Listeria/classification/*genetics/pathogenicity Listeria monocytogenes/genetics/pathogenicity Molecular Sequence Data Multigene Family Nucleic Acid Conformation Peptide Termination Factors Polymerase Chain Reaction Repressor Proteins/genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity Trans-Activators/physiology Virulence
Remarks: We have recently cloned and characterized the inlC gene of Listeria monocytogenes which belongs to the listerial internalin multigene family and codes for a 30-kDa secreted protein containing five consecutive leucine-rich repeats. Here, we show that in L. monocytogenes inlC is located between the rplS gene (encoding the 50S ribosomal protein L19), and the infC gene (encoding the translation initiation factor 3). By direct and inverse polymerase chain reactions (PCR), we cloned a 5.4-kb region containing a homologous gene (termed i-inlC) from L. ivanovii, the other pathogenic member of the genus Listeria. In this microorganism, the i-inlC gene is preceded by another internalin gene, i-inlD, which seems to be specific for L. ivanovii, as this gene could not be detected in L. monocytogenes by Southern hybridization with an i-inlD gene probe. The i-inlD gene also encodes a small secretory internalin (i-InlD), which shares extended homology with (i-)InlC. Upstream of i-inlD are genes for 23S rRNA and 5S rRNA, and two tRNA genes [Asn-tDNA (GTT) and Thr-tDNA(GTT)]. The 3' terminus of the Thr-tRNA gene appears to be the site of an insertion of a genetic element including i-inlC and i-inlD. A putative transcriptional regulator gene, the product of which contains the TetR family signature, is located downstream of i-inlC. This chromosomal position of the two inlC genes on their respective chromosomes may be due to horizontal transfer of this gene. Transcription of i-inlC and i-inlD is strictly dependent on the transcriptional activator PrfA, which regulates transcription of most of the known virulence genes (including inlC) of L. monocytogenes and of L. ivanovii.
URL: 9491077
Ref #: 12314
Author(s): Collins,M.D.;Wallbanks,S.;Lane,D.J.;Shah,J.;Nietupski,R.;Smida,J.;Dorsch,M.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA
Volume: 41
Page(s): 240-6
Year: 1991
Keyword(s): GENBANK/X54447 GENBANK/X56148 GENBANK/X56149 GENBANK/X56150 GENBANK/X56151 GENBANK/X56152 GENBANK/X56153 GENBANK/X56154 GENBANK/X56155 GENBANK/X56156 Base Sequence Lactobacillaceae/classification/genetics Listeria/*classification/genetics Listeria monocytogenes/classification/genetics Molecular Sequence Data *Phylogeny RNA, Bacterial/chemistry/metabolism RNA, Ribosomal, 16S/*chemistry/metabolism RNA-Directed DNA Polymerase/*metabolism
Remarks: The phylogenetic interrelationships of members of the genus Listeria were investigated by using reverse transcriptase sequencing of 16S rRNA. The sequence data indicate that at the intrageneric level the genus Listeria consists of the following two closely related but distinct lines of descent: (i) the Listeria monocytogenes group of species (including Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) and (ii) the species Listeria grayi and Listeria murrayi. At the intergeneric level a specific phylogenetic relationship between the genera Listeria and Brochothrix was evident. The sequence data clearly demonstrated that the genus Listeria is phylogenetically remote from the genus Lactobacillus and should not be included in an extended family Lactobacillaceae.
URL: 91307972
Ref #: 13011
Author(s): Graham,T.A.;Golsteyn-Thomas,E.J.;Thomas,J.E.;Gannon,V.P.
Journal: Int J Syst Bacteriol
Title: Inter- and intraspecies comparison of the 16S-23S rRNA operon intergenic spacer regions of six Listeria spp
Volume: 47
Page(s): 863-9
Year: 1997
Keyword(s): GENBANK/U44061 GENBANK/U44062 GENBANK/U57912 GENBANK/U57913 GENBANK/U57914 GENBANK/U57915 GENBANK/U57916 GENBANK/U57917 GENBANK/U57918 GENBANK/U78980 GENBANK/U78981 GENBANK/U78982 GENBANK/U78983 GENBANK/U78984 Animal Comparative Study DNA, Bacterial/analysis Human Listeria/*classification/*genetics Molecular Sequence Data Operon/genetics RNA, Ribosomal, 16S/*analysis RNA, Ribosomal, 23S/*analysis Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't
Remarks: The 16S-23S rRNA intergenic spacer (IGS) regions found in six Listeria species were characterized. PCR amplification of the 16S-23S IGS with a "generic primer" set generated products of about 340 bp (small) and 550 to 590 bp (large) with DNA from all Listeria strains tested. Seven Listeria monocytogenes serotype 4b strains and one L. monocytogenes serotype 4d strain also had an additional PCR product of ca. 360 bp. The 360-bp PCR product from one of these L. monocytogenes serotype 4b strains was identical in nucleotide sequence to the small 340-bp IGS, except that it contained an 18-bp tandem repeat. The small rRNA IGSs of L. innocua, L. ivanovii, L. seeligeri, L. welshimeri, and L. grayi were 83 to 99% homologous to that of L. monocytogenes. The large rRNA IGS of L. monocytogenes was 81 to 96% homologous to those of the other Listeria species and agreed with current taxonomic division among these species. The nucleotide sequences of the central 274 bp of the large rRNA IGS of strains from seven different L. monocytogenes serotypes were highly homologous; however, serotype-specific differences were noted, and four groups were identified within L. monocytogenes based on this analysis.
URL: 97370613
Data: (ATCC 19119) Type strain / D. Jones, University of Leicester in 1984 / Sheep / Seeliger, H.P.R. et al. (1984) Int. J. syst. Bact. 34, 336
Accession Date: 01/01/1984
History: UNIVERSITY OF LEICESTER
Authority: Seeliger et al. 1984 (ALI)
Depositor: JONES D
Taxonomy: TaxLink: S1690 (Listeria ivanovii subsp. ivanovii Seeliger et al. 1984) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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