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Bacteria Collection: Listeria monocytogenes

NCTC Number: NCTC 5214
Current Name: Listeria monocytogenes
Original Strain Reference: 101
Other Collection No: ATCC 19114; WDCM 00110; 101
Previous Catalogue Name: Listeria monocytogenes
Type Strain: No
Family: Listeriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar 4a
Conditions for growth on solid media: blood agar,37, facultative anaerobe
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, brain sheep circling disease
16S rRNA Gene Sequence: >gb|U84148|ATCC 19114| DD 0648|Listeria monocytogenes 16S ribosomal RNA gene, partial sequence.| acacgtgggcaacct...
23S rRNA Gene Sequence: >gb|X85937|ATCC 19114|L.monocytogenes partial 23S rRNA gene (strain ATCC19114).| taaggtttcctgagg...
Bibliography: PATERSON J S 1940 J PATH BACT 51 427
Extended Bibliography: showhide Show bibliography
Ref #: 95441
Author(s): Rasmussen,O.F.;Skouboe,P.;Dons,L.;Rossen,L.;Olsen,J.E.
Journal: Microbiology
Title: Listeria monocytogenes exists in at least three evolutionary lines: evidence from flagellin, invasive associated protein and listeriolysin O genes
Volume: 141 ( Pt 9)
Page(s): 2053-61
Year: 1996
Keyword(s): GENBANK/X85813 GENBANK/X85814 GENBANK/X85815 GENBANK/X85816 GENBANK/X85817 GENBANK/X85818 GENBANK/X85819 GENBANK/X85820 GENBANK/X85821 GENBANK/X85822 GENBANK/X85823 GENBANK/X85824 GENBANK/X85825 GENBANK/X85826 GENBANK/X85827 GENBANK/X85828 GENBANK/X85829 GENBANK/X85830 GENBANK/X85831 GENBANK/X85832 GENBANK/X85833 GENBANK/X85834 GENBANK/X85835 GENBANK/X85836 GENBANK/X85837 GENBANK/X85838 GENBANK/X85839 GENBANK/X85840 GENBANK/X85841 GENBANK/X85842 Animals Bacterial Proteins/*genetics *Bacterial Toxins Bacterial Typing Techniques Base Sequence Evolution, Molecular Flagellin/*genetics *Genes, Bacterial Heat-Shock Proteins/*genetics Hemolysin Proteins Humans Listeria monocytogenes/*classification/genetics Molecular Sequence Data RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Repetitive Sequences, Nucleic Acid Sequence Alignment Sequence Homology, Nucleic Acid Species Specificity
Remarks: Regions of the genes encoding flagellin (flaA), the invasive associated protein (iap), listeriolysin O (hly) and 23S rRNA were sequenced for a range of Listeria monocytogenes isolates of different origin and serotypes. Several nucleotide sequence variations were found in the flaA, iap and hly genes. No differences were found for the rRNA genes, but our approach does not exclude the existence of differences between single copies of these genes. Based on the sequence differences, the L. monocytogenes strains can be divided into three distinct sequence types. Further, the presence of only a small number of sequence differences within each group indicates a strong degree of conservation within the groups. There was a complete correspondence among the groups of strains formed according to the analysis of the flaA, iap and hly genes, and the grouping correlates with serotype, pulsed field gel electrophoretic and multilocus enzyme electrophoretic data. Analysis of the region encoding the threonine-asparagine repeat units in the iap gene revealed some striking features. Sequence type 1 strains were found to have 16-17 repeats, sequence type 2 strains had 16-20 repeats whereas the two sequence type 3 strains analysed had only 11 repeats. Furthermore, within a 19 bp segment there was a 37% difference between the sequences of type 1 and 2 strains and that segment was absent in type 3 strains. Within the threonine-asparagine repeat region the nucleotide differences gave rise to four amino acid changes; however, all were changes among the three amino acids present in the repeat structure indicating a strong selective pressure on the composition of this region.
URL: 7496516
Data: (ATCC 19114) E. G. D. Murray, Montreal in 1937 / Brain, sheep circling disease, New Zealand in 1931 / Serovar 4a / Paterson, J. S. (1940) J. path. Bact. 51, 427
Accession Date: 01/01/1937
History: ISOLATED BY GILL A NEW ZEALAND 1931-MURRAY E G D CANADA
Authority: (Murray et al. 1926) Pirie 1940 (AL)
Depositor: MURRAY E G D
Taxonomy: TaxLink: S1691 (Listeria monocytogenes (Murray et al. 1926) Pirie 1940) - Date of change: 5/02/2003
Other: IMPORTANT NOTICE: Listeria monocytogenesThe Type Strain of L. monocytogenes is NCTC 10357, but it is atypical in the CAMP test (with Staphylococcus aureus NCTC 1803) and is non-haemolytic: phenotypically, it identifies as Listeria innocua. This culture is also non-motile. For a typical strain of L. monocytogenes we recommend NCTC 7973. (See also: Jones, D. & Seeliger, H. P. R. (1983) Int. J. syst. Bact 33, 429)
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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