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Bacteria Collection: Acinetobacter lwoffii

NCTC Number: NCTC 5866
Current Name: Acinetobacter lwoffii
Original Strain Reference: Lwoff (1)
Other Collection No: ATCC 15309; CIP 644.10; DSM 2403; LWOFF(1)
Previous Catalogue Name: Acinetobacter lwoffii
Other Names: MORAXELLA LWOFFII VAR BREVIS, (AUDUREAU, 1940) BRISOU AND PREVOT, 1954
Type Strain: Yes
Family: Moraxellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1068200
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ247205|DSM 2403|Acinetobacter lwoffii 16S rRNA gene, partial.| catgcaagtcgagcg... >gb|X81665|DSM 2403|A.lwoffii 16S rRNA gene (DSM2403).| aacgctggcggcagg...
23S rRNA Gene Sequence: >gb|AF124616|DSM 2403|Acinetobacter lwoffii strain DSM 2403 23S ribosomal RNA gene,partial sequence.| aatacttctgtgtaa...
Bibliography: AUDUREAU A 1940 ANNLS INST PASTEUR PARIS 64 126; STEEL K & COWAN S T 1964
Extended Bibliography: showhide Show bibliography
Ref #: 15329
Author(s): Rainey,F.A.;Lang,E.;Stackebrandt,E.
Journal: FEMS Microbiol Lett
Title: The phylogenetic structure of the genus Acinetobacter
Volume: 124
Page(s): 349-53
Year: 1995
Keyword(s): GENBANK/X81657 GENBANK/X81658 GENBANK/X81659 GENBANK/X81660 GENBANK/X81661 GENBANK/X81662 GENBANK/X81663 GENBANK/X81664 GENBANK/X81665 GENBANK/X81666 GENBANK/X81667 GENBANK/X81668 Acinetobacter/*classification/genetics DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Sequence Analysis, DNA
Remarks: 16S rDNA sequence analysis was performed on the type strains of all validly described Acinetobacter species and five unnamed Acinetobacter strains. The phylogenetic analyses confirm that Acinetobacter is a coherent genus within the gamma subclass of Proteobacteria and that the species are phylogenetically well defined. A. calcoaceticus, A. lwoffii, A. johnsonii and A. haemolyticus form one cluster of closely related species, the pair A. junii and A. baumannii forms a second cluster. A. radioresistens stands phylogenetically isolated. The study reveals that three undescribed strains can be assigned to individually described species, while strains DSM 30009 and DSM 590 may represent two novel Acinetobacter species.
URL: 7851741
Ref #: 15318
Author(s): Yamamoto,S.;Harayama,S.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of Acinetobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products
Volume: 46
Page(s): 506-11
Year: 1996
Keyword(s): GENBANK/D73412 GENBANK/D73413 GENBANK/D73414 GENBANK/D73415 GENBANK/D73416 GENBANK/D73417 GENBANK/D73418 GENBANK/D73419 GENBANK/D73420 GENBANK/D73421 GENBANK/D73422 GENBANK/D73423 GENBANK/D73424 GENBANK/D73425 GENBANK/D73426 GENBANK/D73427 GENBANK/D73428 GENBANK/D73429 GENBANK/D73430 GENBANK/D73431 GENBANK/D73432 GENBANK/D73433 GENBANK/D73434 GENBANK/D73435 GENBANK/D73436 GENBANK/D73437 GENBANK/D73438 GENBANK/D73439 GENBANK/D73440 GENBANK/D73441 Acinetobacter/classification/*genetics Amino Acid Sequence Bacterial Proteins/genetics DNA Gyrase DNA Topoisomerases, Type II/*genetics Genes, Bacterial Molecular Sequence Data Phylogeny
Remarks: Partial nucleotide sequences of the gyrB genes (DNA gyrase B subunit genes) of 15 Acinetobacter strains, including the type and reference strains of genomic species 1 to 12 (A. calcoaceticus [genomic species 1], A. baumannii [genomic species 2], Acinetobacter genomic species 3, A. haemolyticus [genomic species 4], A. junii [genomic species 5], Acinetobacter genomic species 6, A. johnsonii [genomic species 7], A. lwoffii [genomic species 8], Acinetobacter genomic species 9, Acinetobacter genomic species 10, Acinetobacter genomic species 11, and A. radioresistens [genomic species 12]), were determined by sequencing the PCR-amplified fragments of gyrB. The gyrB sequence homology among these Acinetobacter strains ranged from 69.6 to 99.7%. A phylogenetic analysis, using the gyrB sequences, indicates that genomic species 1, 2, and 3 formed one cluster (87.3 to 90.3% identity), while genomic species 8 and 9 formed another cluster (99.7% identity). These results are consistent with those of DNA-DNA hybridization and of biochemical systematics. On the other hand, the topology of the published phylogenetic tree based on the 16S rRNA sequences of the Acinetobacter strains was quite different from that of the gyrB-based tree. The numbers of substitution in the 16S rRNA gene sequences were not high enough to construct a reliable phylogenetic tree. The gyrB-based analysis indicates that the genus Acinetobacter is highly diverse and that a reclassification of this genus would be required.
URL: 8934907
Ref #: 15405
Author(s): Yamamoto,S.;Bouvet,P.J.;Harayama,S.
Journal: Int J Syst Bacteriol
Title: Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization
Volume: 49 Pt 1
Page(s): 87-95
Year: 1999
Keyword(s): GENBANK/AB008684 GENBANK/AB008685 GENBANK/AB008686 GENBANK/AB008687 GENBANK/AB008688 GENBANK/AB008689 GENBANK/AB008690 GENBANK/AB008691 GENBANK/AB008692 GENBANK/AB008693 GENBANK/AB008694 GENBANK/AB008695 GENBANK/AB008696 GENBANK/AB008697 GENBANK/AB008698 GENBANK/AB008699 GENBANK/AB008700 GENBANK/AB008701 GENBANK/AB008702 GENBANK/AB008703 GENBANK/AB008704 GENBANK/AB008705 GENBANK/AB008706 GENBANK/AB008707 GENBANK/AB008708 GENBANK/AB008709 GENBANK/AB008710 GENBANK/AB008711 GENBANK/AB008712 GENBANK/AB008713 Acinetobacter/*classification/genetics Base Sequence DNA Gyrase DNA Topoisomerases, Type II/*genetics DNA, Bacterial/*analysis Molecular Sequence Data *Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S/chemistry
Remarks: The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.
URL: 10028249
Ref #: 11981
Author(s): Rainey,F.A.;Lang,E.;Stackebrandt,E.
Journal: FEMS Microbiol Lett
Title: The phylogenetic structure of the genus Acinetobacter
Volume: 124
Page(s): 349-53
Year: 1995
Keyword(s): GENBANK/X81657 GENBANK/X81658 GENBANK/X81659 GENBANK/X81660 GENBANK/X81661 GENBANK/X81662 GENBANK/X81663 GENBANK/X81664 GENBANK/X81665 GENBANK/X81666 GENBANK/X81667 GENBANK/X81668 Acinetobacter/*classification/genetics DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Sequence Analysis, DNA
Remarks: 16S rDNA sequence analysis was performed on the type strains of all validly described Acinetobacter species and five unnamed Acinetobacter strains. The phylogenetic analyses confirm that Acinetobacter is a coherent genus within the gamma subclass of Proteobacteria and that the species are phylogenetically well defined. A. calcoaceticus, A. lwoffii, A. johnsonii and A. haemolyticus form one cluster of closely related species, the pair A. junii and A. baumannii forms a second cluster. A. radioresistens stands phylogenetically isolated. The study reveals that three undescribed strains can be assigned to individually described species, while strains DSM 30009 and DSM 590 may represent two novel Acinetobacter species.
URL: 95154670
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 4025
Author(s): Bouvet,P.J.M.;Grimont,P.A.D.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and emended descriptions of Acinetobacter calcoaceticus
Volume: 36
Page(s): 228-240
Year: 1986
Ref #: 6586
Author(s): Rainey,F.A.;Lang,E.;Stackebrandt,E.
Journal: FEMS Microbiol. Lett.
Title: The phylogenetic structure of the genus Acinetobacter.
Volume: 124
Page(s): 349-354
Year: 1994
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 15309) Type strain / A. Lwoff, Paris in 1939 / Moraxella lwoffii var. brevis / Audureau, A. (1940) Annls. Inst. Pasteur, Paris, 64, 126 / Steel, K. & Cowan, S. T. (1964) Annls. Inst. Pasteur, Paris 106, 479
Accession Date: 01/01/1939
History: LWOFF A PASTEUR INSTITUTE PARIS
Authority: (Audureau 1940) Brisou and Prévot 1954 emend. Bouvet and Grimont 1986
Depositor: LWOFF A
Taxonomy: TaxLink: S75 (Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 emend. Bouvet and Grimont 1986) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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