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Bacteria and Mycoplasmas detail

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Bacteria Collection: Enterococcus faecalis

NCTC Number: NCTC 775
Current Name: Enterococcus faecalis
Original Strain Reference: Tissier
Other Collection No: ATCC 19433; DSM 20478; NCDO 581; NCIB 775; WDCM 00009
Previous Catalogue Name: Enterococcus faecalis
Type Strain: Yes
Family: Enterococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group d
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1043812
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|Y12905|ATCC 19433 (CCUG 19916)|E.faecalis 16S rRNA gene, partial (strain ATCC 19433 (CCUG 19916)).| atcctttgaccactc... >gb|DQ411814|ATCC 19433|Enterococcus faecalis strain ATCC 19433 16S ribosomal RNA gene,partial sequence.| gacgaacgctggcgg... >gb|AY351321|ATCC 19433|Enterococcus faecalis strain ATCC 19433 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ctaaggaatattacg... >gb|D31675|NCDO581|Enterococcus faecalis gene for 16S ribosomal RNA, partial sequence.| gggatgacgtcaaat... >gb|X87182|ATCC 19433|E.faecalis 16S-23S rRNA spacer DNA.| ctaaggaatattacg...
23S rRNA Gene Sequence: >gb|AY351321|ATCC 19433|Enterococcus faecalis strain ATCC 19433 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ctaaggaatattacg... >gb|X87182|ATCC 19433|E.faecalis 16S-23S rRNA spacer DNA.| ctaaggaatattacg...
Bibliography: INSTITUT PASTEUR PARIS 1949 SHATTOCK P M F J GEN MICROBIOL 3 80
Extended Bibliography: showhide Show bibliography
Ref #: 43856
Author(s): Naimi,A.;Beck,G.;Branlant,C.
Journal: Microbiology
Title: Primary and secondary structures of rRNA spacer regions in enterococci
Volume: 143 ( Pt 3)
Page(s): 823-34
Year: 1997
Keyword(s): GENBANK/X87177 GENBANK/X87178 GENBANK/X87179 GENBANK/X87180 GENBANK/X87181 GENBANK/X87182 GENBANK/X87183 GENBANK/X87184 GENBANK/X87185 GENBANK/X87186 GENBANK/X87187 GENBANK/X87188 GENBANK/X87189 GENBANK/X87190 GENBANK/X87191 Base Sequence Enterococcus/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry/*genetics RNA, Ribosomal/chemistry/*genetics RNA, Transfer/genetics Sequence Alignment
Remarks: The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
URL: 9084166
Ref #: 95521
Author(s): Monstein,H.J.;Quednau,M.;Samuelsson,A.;Ahrne,S.;Isaksson,B.;Jonasson,J.
Journal: Microbiology
Title: Division of the genus Enterococcus into species groups using PCR-based molecular typing methods
Volume: 144 ( Pt 5)
Page(s): 1171-9
Year: 1998
Keyword(s): GENBANK/Y12905 GENBANK/Y12906 GENBANK/Y12907 GENBANK/Y12908 GENBANK/Y12909 GENBANK/Y12910 GENBANK/Y12911 GENBANK/Y12912 GENBANK/Y12913 GENBANK/Y12914 GENBANK/Y12915 GENBANK/Y12916 GENBANK/Y12917 GENBANK/Y12918 GENBANK/Y12919 GENBANK/Y12920 GENBANK/Y12921 GENBANK/Y12922 GENBANK/Y12923 GENBANK/Y12924 *Bacterial Typing Techniques Base Sequence Blotting, Southern DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Electrophoresis, Agar Gel Enterococcus/*classification/genetics/isolation & purification Epidemiology, Molecular Gram-Positive Bacterial Infections/*microbiology Humans Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Random Amplified Polymorphic DNA Technique Sequence Alignment Sequence Analysis, DNA Species Specificity
Remarks: Broad-range 16S rDNA PCR (BR-PCR) applied to DNA from 32 clinical enterococcal isolates and 12 other enterococci from a clinical reference collection followed by species-specific hybridization analysis identified 25 strains of Enterococcus faecalis and 19 Enterococcus species. Randomly amplified polymorphic DNA (RAPD) analysis using UPGMA clustering on the same material revealed four different clusters at a similarity level of 49%. Based on partial 16S rDNA sequence analysis of variable regions V4 and V9, it was possible to divide the 19 type strains specifying the genus Enterococcus into 12 different 16S rDNA species groups. The type strain distribution then served as a template for the analysis of the other 44 strains which were assigned to four different species groups (a-d) based on their 16S rDNA motifs. There was good agreement with the RAPD clusters. Species group a was an individual species line containing 25 strains that were identified as E. faecalis. Group b also represented an individual species line of 12 strains identified as E. faecium. The remaining seven strains that formed species groups c and d could not be fully identified to species by this analysis. It was concluded that BR-PCR of 16S rDNA followed by partial sequence analysis of the PCR products is a reliable technique for the identification and classification of enterococci. Further division of unresolved species groups should be achievable if regions other than V4 and V9 of 16S rDNA are also analysed.
URL: 9611791
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 13717
Author(s): Monstein,H.J.;Quednau,M.;Samuelsson,A.;Ahrne,S.;Isaksson,B.;Jonasson,J.
Journal: Microbiology
Title: Division of the genus Enterococcus into species groups using PCR-based molecular typing methods
Volume: 144 ( Pt 5)
Page(s): 1171-9
Year: 1998
Keyword(s): GENBANK/Y12905 GENBANK/Y12906 GENBANK/Y12907 GENBANK/Y12908 GENBANK/Y12909 GENBANK/Y12910 GENBANK/Y12911 GENBANK/Y12912 GENBANK/Y12913 GENBANK/Y12914 GENBANK/Y12915 GENBANK/Y12916 GENBANK/Y12917 GENBANK/Y12918 GENBANK/Y12919 GENBANK/Y12920 GENBANK/Y12921 GENBANK/Y12922 GENBANK/Y12923 GENBANK/Y12924 *Bacterial Typing Techniques Base Sequence Blotting, Southern DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Electrophoresis, Agar Gel Enterococcus/*classification/genetics/isolation & purification Epidemiology, Molecular Gram-Positive Bacterial Infections/*microbiology Human Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Random Amplified Polymorphic DNA Technique Sequence Alignment Sequence Analysis, DNA Species Specificity Support, Non-U.S. Gov't
Remarks: Broad-range 16S rDNA PCR (BR-PCR) applied to DNA from 32 clinical enterococcal isolates and 12 other enterococci from a clinical reference collection followed by species-specific hybridization analysis identified 25 strains of Enterococcus faecalis and 19 Enterococcus species. Randomly amplified polymorphic DNA (RAPD) analysis using UPGMA clustering on the same material revealed four different clusters at a similarity level of 49%. Based on partial 16S rDNA sequence analysis of variable regions V4 and V9, it was possible to divide the 19 type strains specifying the genus Enterococcus into 12 different 16S rDNA species groups. The type strain distribution then served as a template for the analysis of the other 44 strains which were assigned to four different species groups (a-d) based on their 16S rDNA motifs. There was good agreement with the RAPD clusters. Species group a was an individual species line containing 25 strains that were identified as E. faecalis. Group b also represented an individual species line of 12 strains identified as E. faecium. The remaining seven strains that formed species groups c and d could not be fully identified to species by this analysis. It was concluded that BR-PCR of 16S rDNA followed by partial sequence analysis of the PCR products is a reliable technique for the identification and classification of enterococci. Further division of unresolved species groups should be achievable if regions other than V4 and V9 of 16S rDNA are also analysed.
URL: 98274725
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2392
Author(s): Ottogalli,G.;Galli,A.;Dellaglio,F.
Journal: J. Dairy Res.
Title: Taxonomic relationships between Streptococcus thermophilus and some other streptococci.
Volume: 46
Page(s): 127-131
Year: 1979
Ref #: 2681
Author(s): Schleifer,K.H.;Kilpper-Bälz,R.
Journal: Int. J. Syst. Bacteriol.
Title: Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.
Volume: 34
Page(s): 31-34
Year: 1984
Data: (ATCC 19433, DSM 20478) Type strain / C. L. Hannay, NIRD Shinfield in 1950 / Enterococcus Tissier from Institut Pasteur, Redeposition of original strain from H. C. Brown, Wellcome Bureau of Scientific Research in 1950 / Institut Pasteur, Paris / Shattock, P. M. F. (1949) J. gen. Microbiol. 3, 80
Accession Date: 01/01/1950
History: PASTEUR INST 1920 - BROWN H C WELLCOME - REDEPOSITED 1950 PRE:FR
Authority: (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984
Depositor: HANNAY C L
Taxonomy: TaxLink: S1144 (Streptococcus faecalis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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