Bacteria Collection: Listeria monocytogenes

NCTC Number:	NCTC 7973
Current Name:	Listeria monocytogenes
Original Strain Reference:	58 xxiii
Other Collection No:	ATCC 35152; CCM 5576; DSM 12464; SLCC 2371; WDCM 00109; 58 XXIII
Previous Catalogue Name:	Listeria monocytogenes
Type Strain:	No
Family:	Listeriaceae
Hazard Group (ACDP):	2
Release Restrictions:	Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties:	serovar 1a
Conditions for growth on solid media:	blood agar,37, facultative anaerobe
Conditions for growth on liquid media:	nutrient broth,37, facultative anaerobe
Isolated From:	mammal, guinea pig mesenteric lymph node
Bibliography:	MURRAY E G D ET AL 1926 J PATH BACT 29 407;MURRAY E G D 1963 2ND SYMPOSIUM
Extended Bibliography:	Show bibliography Ref #: 95463 Author(s): Morse,R.;O'Hanlon,K.;Virji,M.;Collins,M.D. Journal: J Clin Microbiol Title: Isolation of rifampin-resistant mutants of Listeria monocytogenes and their characterization by rpoB gene sequencing, temperature sensitivity for growth, and interaction with an epithelial cell line Volume: 37 Page(s): 2913-9 Year: 1999 Keyword(s): GENBANK/Y16468 Antibiotics, Antitubercular/*pharmacology Base Sequence Caco-2 Cells/microbiology Drug Resistance, Microbial *Genes, Bacterial Humans Listeria monocytogenes/drug effects/*genetics/growth & development Microbial Sensitivity Tests Molecular Sequence Data Mutation Plant Proteins/*genetics Rifampin/*pharmacology Temperature Remarks: The sequence of the rpoB gene from Listeria monocytogenes was determined. Rifampin-resistant (Rif(r)) mutants arising from L. monocytogenes cultures exposed to rifampin were isolated, and by partial sequencing of their rpoB genes, seven different point mutations affecting five different amino acids (473Asp-->Asn or Gly, 479Gly-->Asp, 483His-->Tyr or Leu, 528Ile-->Phe, and 530Ser-->Tyr), which led to MICs of 0.5 to 100 microg/ml for the organisms, were determined. These mutants showed various deficiencies for growth at 42 degrees C, with only one being comparable to the wild-type strain. The interaction of these Rif(r) mutants with human Caco-2 cells was examined by using an immunofluorescence technique. Three mutants failed to interact, while three showed a reduced interaction compared to that of the wild type. It is believed that these pleiotropic phenotypes have arisen as a result of mutations within the DNA-dependent RNA polymerase holoenzyme. URL: 10449475 Ref #: 69143 Author(s): Morse,R.;O'Hanlon,K.;Collins,M.D. Journal: Int J Syst Evol Microbiol Title: Phylogenetic, amino acid content and indel analyses of the beta subunit of DNA-dependent RNA polymerase of gram-positive and gram-negative bacteria Volume: 52 Page(s): 1477-84 Year: 2002 Keyword(s): GENBANK/AE001944 GENBANK/Y16466 GENBANK/Y16467 GENBANK/Y16468 GENBANK/Y16469 GENBANK/Y16470 GENBANK/Y16471 GENBANK/Y16472 GENBANK/Y19223 Amino Acid Sequence Amino Acids/analysis Base Composition Base Sequence DNA Transposable Elements DNA, Bacterial/chemistry/genetics DNA-Directed RNA Polymerases/*chemistry/*genetics Drug Resistance, Bacterial/genetics Genes, Bacterial Gram-Negative Bacteria/classification/*enzymology/*genetics Gram-Positive Bacteria/classification/*enzymology/*genetics Molecular Sequence Data Phylogeny Protein Subunits RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Rifampin/pharmacology Sequence Deletion Sequence Homology, Amino Acid Remarks: In this study, we have sequenced the rpoB gene, encoding the beta subunit of DNA-dependent RNA polymerase, from a selection of gram-positive and gram-negative bacteria. The presence of insertions and deletions (indels) in the beta subunit separate the gram-positive and gram-negative bacteria from each other and support the division of the gram-positive organisms into two clades based on DNA G+C content. Phylogenetic and amino acid content analyses further separate the clostridia from bacilli, leuconostocs, listeriae and relatives, forming an early branch after the common gram-positive ancestor. The occurrence in the beta subunit of Asn-Ala at positions 471-472 in Porphyromonas cangingivalis and Asn at position 372 in Weissella paramesenteroides are postulated to be the cause of the natural rifampicin resistance of these species. URL: 12361249
Data:	(ATCC 35152) R. A. Webb, London in 1949 / Guinea-pig mesenteric lymph node in 1924 / Serovar 1/2a / Murray, E. G. D. et al. (1926) J. path. Bact. 29, 407 / Murray, E. G. D. (1963) Second Symposium on Listeric Infection, Ed. M. L. Gray, Montana State College, Bozeman / Spontaneously produces non-haemolytic variants due to deletion of part prfA gene which controls a series of genes associated with virulence / Pine, L. et al. J. clin. Microbiol. (1987) 25, 2247 / Gormley et al. (1987) Res. Microbiol. 140, 631 / Leimeister-Wachter, M. & Chakraborty, T. (1989) Infect. Immun. 57, 2350 / Portnoy, D.A. et al. Infect. Immun. (1992) 60, 1263
Accession Date:	01/01/1949
Authority:	(Murray et al. 1926) Pirie 1940 (AL)
Depositor:	WEBB R A
Taxonomy:	TaxLink: S1691 (Listeria monocytogenes (Murray et al. 1926) Pirie 1940) - Date of change: 5/02/2003
Other:	IMPORTANT NOTICE: Listeria monocytogenesThe Type Strain of L. monocytogenes is NCTC 10357, but it is atypical in the CAMP test (with Staphylococcus aureus NCTC 1803) and is non-haemolytic: phenotypically, it identifies as Listeria innocua. This culture is also non-motile. For a typical strain of L. monocytogenes we recommend NCTC 7973. (See also: Jones, D. & Seeliger, H. P. R. (1983) Int. J. syst. Bact 33, 429)
Biosafety Responsibility:	It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.