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Bacteria and Mycoplasmas detail

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Bacteria Collection: Streptococcus agalactiae

NCTC Number: NCTC 8181
Current Name: Streptococcus agalactiae
Original Strain Reference: G 19
Other Collection No: ATCC 13813; DSM 2134; G 19
Previous Catalogue Name: Streptococcus agalactiae
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group b
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: milk
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1003590
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AB002479|ATCC 13813| NCTC 8181|Streptococcus agalactiae DNA for 16S rRNA, strain ATCC 13813, NCTC8181.| agaacgcgaggttgg... >gb|U02908|ATCC 13813|Streptococcus agalactiae ATCC 13813 16S rRNA gene, partialsequence.| ttgccatcattaagt... >gb|DQ303183|ATCC 13813|Streptococcus agalactiae strain ATCC 13813 16S ribosomal RNA gene,partial sequence.| ctggcggcgtgccta... >gb|AY347539|ATCC 13813|Streptococcus agalactiae strain ATCC 13813 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggataaggaaacc...
23S rRNA Gene Sequence: >gb|AB096754|ATCC 13813|Streptococcus agalactiae gene for 23S rRNA, complete sequence,strain:ATCC 13813.| ggttaagttaataag... >gb|AY347539|ATCC 13813|Streptococcus agalactiae strain ATCC 13813 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggataaggaaacc...
Bibliography: LANCEFIELD R C 1934 J EXP MED 59 441;SUBCOMMITEE 1963 INT BULL BACT NOM-
Extended Bibliography: showhide Show bibliography
Ref #: 19216
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid
Volume: 32
Page(s): 335-51
Year: 1994
Keyword(s): GENBANK/U02893 GENBANK/U02894 GENBANK/U02895 GENBANK/U02896 GENBANK/U02897 GENBANK/U02898 GENBANK/U02899 GENBANK/U02900 GENBANK/U02901 GENBANK/U02902 GENBANK/U02903 GENBANK/U02904 GENBANK/U02905 GENBANK/U02906 GENBANK/U02907 GENBANK/U02908 GENBANK/U02909 GENBANK/U02910 GENBANK/U02911 GENBANK/U02912 GENBANK/U02913 GENBANK/U02914 GENBANK/U02915 GENBANK/U02916 GENBANK/U02917 GENBANK/U02918 GENBANK/U02919 GENBANK/U02920 GENBANK/U02921 GENBANK/U02922 Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Humans Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF.
URL: 7512093
Ref #: 83019
Author(s): Kawata,K.;Minakami,T.;Mori,Y.;Katsumi,M.;Kataoka,Y.;Ezawa,A.;Kikuchi,N.;Takahashi,T.
Journal: Int J Syst Evol Microbiol
Title: rDNA sequence analyses of Streptococcus dysgalactiae subsp. equisimilis isolates from pigs
Volume: 53
Page(s): 1941-6
Year: 2003
Keyword(s): Animals Base Sequence DNA Primers DNA, Bacterial/genetics DNA, Ribosomal/*genetics *Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Alignment Sequence Homology, Nucleic Acid Streptococcus/*classification/*genetics/isolation & purification Swine/*microbiology
Remarks: The nucleotide sequences of 16S and 23S rRNA genes (rDNA) were determined for 11 isolates of Streptococcus dysgalactiae subsp. equisimilis from slaughtered pigs with endocarditis, arthritis or lymphadenitis and strain ATCC 35666, designated as a strain of subspecies equisimilis. The sequences of each of the genes were compared phylogenetically with the corresponding sequences of S. dysgalactiae subsp. dysgalactiae ATCC 43078(T) and ATCC 27957, which were also determined in this study. Based on the 16S rDNA analysis, the isolates of S. dysgalactiae subsp. equisimilis were divided into two distinct groups, designated groups 1 and 2. S. dysgalactiae subsp. equisimilis ATCC 35666 was closely related to the group 2 strains. The S. dysgalactiae subsp. dysgalactiae strains seemed to be associated with the group 1 strains, which was not consistent with the conventional subspecific classification of S. dysgalactiae. In contrast, the 23S rDNA analysis distinguished S. dysgalactiae subsp. dysgalactiae strains from subsp. equisimilis strains. This inconsistency between phylogenies based on 16S and 23S rDNA indicates that 23S rDNA is a more rigid marker for determining the phylogenetic relationships and taxonomic position of these organisms than is 16S rDNA.
URL: 14657127
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 84110
Author(s): Bekal,S.;Gaudreau,C.;Laurence,R.A.;Simoneau,E.;Raynal,L.
Journal: J Clin Microbiol
Title: Streptococcus pseudoporcinus sp. nov., a novel species isolated from the genitourinary tract of women
Volume: 44
Page(s): 2584-6
Year: 2006
Keyword(s): GENBANK/DQ303183 GENBANK/DQ303184 GENBANK/DQ303185 GENBANK/DQ303186 GENBANK/DQ303187 GENBANK/DQ303188 GENBANK/DQ303189 GENBANK/DQ303190 GENBANK/DQ303191 GENBANK/DQ303192 GENBANK/DQ303193 GENBANK/DQ303194 GENBANK/DQ303195 GENBANK/DQ303196 GENBANK/DQ303197 GENBANK/DQ303198 GENBANK/DQ303199 GENBANK/DQ303200 GENBANK/DQ303201 GENBANK/DQ303202 GENBANK/DQ303203 GENBANK/DQ303204 GENBANK/DQ303205 GENBANK/DQ303206 GENBANK/DQ303207 GENBANK/DQ303208 GENBANK/DQ303209 GENBANK/DQ340843 Cluster Analysis DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Female Female Urogenital Diseases/*microbiology Genes, rRNA Humans Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Streptococcal Infections/*microbiology Streptococcus/*classification/genetics/*isolation & purification Urogenital System/*microbiology
Remarks: Streptococcus strains from animal and human sources identified biochemically as Streptococcus porcinus were investigated by 16S rRNA gene sequencing. The nine human strains isolated between 1997 and 2005 formed a single cluster with more than 2.1% dissimilarity with S. porcinus strains from animal sources. A novel species, Streptococcus pseudoporcinus sp. nov., is proposed.
URL: 16825387
Ref #: 13687
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic
Volume: 32
Page(s): 335-351
Year: 1994
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Human Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were
URL: 94201356
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 13813, DSM 2134) Type strain / A. W. Stableforth, Weybridge in 1949 / Milk, N. S. W. / Lancefield, R. C. (1934) J. exp. Med. 59, 441 / Stewart, D. F. (1937) J. path. Bact 45, 279 / Subcommittee (1961) Int. Bull. bact. Nomencl. Taxon. 11, 21; (1963) 13, 37
Accession Date: 01/01/1949
Authority: Lehmann and Neumann 1896 (AL)
Depositor: STABLEFORTH A W
Taxonomy: TaxLink: S2822 (Streptococcus agalactiae Lehmann and Neumann 1896) - Date of change: 5/02/2003
Other: Stableforths (1932, J. comp. Path. Ther. 45, 185; 1937, p. Path. Bact. 45, 163)Serological Group BType1c
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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