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Bacteria and Mycoplasmas detail

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Bacteria Collection: Klebsiella pneumoniae subsp. pneumoniae

NCTC Number: NCTC 9633
Current Name: Klebsiella pneumoniae subsp. pneumoniae
Original Strain Reference: CDC 298/53
Other Collection No: ATCC 13883; CDCD 298/53; DSM 30104; IAM 14200; JCM 1662; WDCM 00097
Previous Catalogue Name: Klebsiella pneumoniae
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|AF130981|ATCC13883|Klebsiella pneumoniae 16S ribosomal RNA gene, partial sequence.| atcctggctcagatt... >gb|AJ233420|DSM 30104|Klebsiella pneumoniae 16S rRNA gene (strain DSM 30104).| ggcaggcctaacaca... >gb|Y17656|ATCC13883T|Klebsiella pneumoniae 16S rRNA gene, strain ATCC13883T, partial.| tgaacgctggcggca... >gb|AB004753|JCM1662|Klebsiella pneumoniae gene for 16S ribosomal RNA, partial sequence.| acgctggcggcaggc... >gb|X87276|DSM 30104 T|K.pneumoniae 16S rRNA gene.| agagtttgatnntgg...
23S rRNA Gene Sequence: >gb|X87284|ATCC 13883 T|K.pneumoniae 23S rRNA gene, ATCC 13883 T.| ggttaagcgactaag... >gb|AJ549512|TYPE STRAIN: DSM 30104|Klebsiella pneumoniae subsp. pneumoniae partial 23S rRNA gene, typestrain DSM 30104T.| catttaaagaaagcg...
Bibliography: COWAN S T ET AL 1960 J GEN MICROBIOL 23 601
Extended Bibliography: showhide Show bibliography
Ref #: 45324
Author(s): Sproer,C.;Mendrock,U.;Swiderski,J.;Lang,E.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae
Volume: 49 Pt 4
Page(s): 1433-8
Year: 1999
Keyword(s): GENBANK/AJ233400 GENBANK/AJ233401 GENBANK/AJ233402 GENBANK/AJ233403 GENBANK/AJ233404 GENBANK/AJ233405 GENBANK/AJ233406 GENBANK/AJ233407 GENBANK/AJ233408 GENBANK/AJ233409 GENBANK/AJ233410 GENBANK/AJ233411 GENBANK/AJ233412 GENBANK/AJ233413 GENBANK/AJ233414 GENBANK/AJ233415 GENBANK/AJ233416 GENBANK/AJ233417 GENBANK/AJ233418 GENBANK/AJ233419 GENBANK/AJ233420 GENBANK/AJ233421 GENBANK/AJ233422 GENBANK/AJ233423 GENBANK/AJ233424 GENBANK/AJ233425 GENBANK/AJ233426 GENBANK/AJ233427 GENBANK/AJ233428 GENBANK/AJ233429 etc. DNA, Ribosomal/genetics Enterobacteriaceae/classification/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Serratia/classification/*genetics
Remarks: The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis. Several sequences of strains from the genera Citrobacter, Erwinia, Pantoea, Proteus, Rahnella and Serratia, analysed in this study, have been analysed previously. However, as the sequences of this study differ slightly from the published ones, they were included in the analysis. Of the 23 enterobacterial genera included in an overview dendrogram of relatedness, members of the genera Xenorhabdus, Photorhabdus, Proteus and Plesiomonas were used as a root. The other genera formed two groups which could be separated, although not exclusively, by signature nucleotides at positions 590-649 and 600-638. Group A contains species of Brenneria, Buttiauxella, Citrobacter, Escherichia, Erwinia, Klebsiella, Pantoea, Pectobacterium and Salmonella. All seven type strains of Buttiauxella share 16S rDNA similarities greater than 99%. Group B embraces two phylogenetically separate Serratia clusters, a lineage containing Yersinia species, Rahnella aquatica, Ewingella americana, and also the highly related pair Hafnia alvei and Obesumbacterium proteus.
URL: 10555323
Ref #: 50886
Author(s): Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume: 51
Page(s): 925-32
Year: 2001
Keyword(s): GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/*classification/genetics Erwinia/classification/genetics Klebsiella/*classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks: The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL: 11411716
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 12300
Author(s): Sproer,C.;Mendrock,U.;Swiderski,J.;Lang,E.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae
Volume: 49 Pt 4
Page(s): 1433-8
Year: 1999
Keyword(s): GENBANK/AJ233400 GENBANK/AJ233401 GENBANK/AJ233402 GENBANK/AJ233403 GENBANK/AJ233404 GENBANK/AJ233405 GENBANK/AJ233406 GENBANK/AJ233407 GENBANK/AJ233408 GENBANK/AJ233409 GENBANK/AJ233410 GENBANK/AJ233411 GENBANK/AJ233412 GENBANK/AJ233413 GENBANK/AJ233414 GENBANK/AJ233415 GENBANK/AJ233416 GENBANK/AJ233417 GENBANK/AJ233418 GENBANK/AJ233419 GENBANK/AJ233420 GENBANK/AJ233421 GENBANK/AJ233422 GENBANK/AJ233423 GENBANK/AJ233424 GENBANK/AJ233425 GENBANK/AJ233426 GENBANK/AJ233427 GENBANK/AJ233428 GENBANK/AJ233429 etc. DNA, Ribosomal/genetics Enterobacteriaceae/classification/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Serratia/classification/*genetics
Remarks: The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis. Several sequences of strains from the genera Citrobacter, Erwinia, Pantoea, Proteus, Rahnella and Serratia, analysed in this study, have been analysed previously. However, as the sequences of this study differ slightly from the published ones, they were included in the analysis. Of the 23 enterobacterial genera included in an overview dendrogram of relatedness, members of the genera Xenorhabdus, Photorhabdus, Proteus and Plesiomonas were used as a root. The other genera formed two groups which could be separated, although not exclusively, by signature nucleotides at positions 590-649 and 600-638. Group A contains species of Brenneria, Buttiauxella, Citrobacter, Escherichia, Erwinia, Klebsiella, Pantoea, Pectobacterium and Salmonella. All seven type strains of Buttiauxella share 16S rDNA similarities greater than 99%. Group B embraces two phylogenetically separate Serratia clusters, a lineage containing Yersinia species, Rahnella aquatica, Ewingella americana, and also the highly related pair Hafnia alvei and Obesumbacterium proteus.
URL: 20023023
Ref #: 12920
Author(s): Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume: 51
Page(s): 925-32
Year: 2001
Keyword(s): GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/*classification/genetics Erwinia/classification/genetics Klebsiella/*classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks: The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL: 21304435
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 13883) Type strain / P. R. Edwards, CDC Atlanta in 1955 / Capsular serotype 3 / Cowan, S. T. et al. (1960) J. gen. Microbiol. 23, 601
Accession Date: 01/01/1955
History: RECEIVED FROM EDWARDS P R CDC ATLANTA
Authority: (Schroeter 1886) Trevisan 1887 (AL)
Depositor: EDWARDS P R
Taxonomy: TaxLink: S1519 (Klebsiella pneumoniae subsp. pneumoniae (Schroeter 1886) Trevisan 1887) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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