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Bacteria Collection: Klebsiella aerogenes

NCTC Number: NCTC 10006
Current Name: Klebsiella aerogenes
Original Strain Reference: CDC 819-56
Other Collection No: ATCC 13048; CDC 819-56; DSM 30053; WDCM 00175
Previous Catalogue Name: Enterobacter aerogenes
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: sputum
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS462975
16S rRNA Gene Sequence: >gb|AJ001237|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 5 end, strainNCTC 10006 T.| cgctggcggcaggct... >gb|AJ001238|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 3 end, NCTC10006 T.| cgatgtcgacttgga... >gb|AJ251468|NCTC10006T|Enterobacter aerogenes partial 16S rRNA gene, strain NCTC10006T.| tgaacgctggcggca... >gb|AJ001237|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 5' end, strainNCTC 10006 T.| cgctggcggcaggct... >gb|AJ001238|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 3' end, NCTC10006 T.| cgatgtcgacttgga...
23S rRNA Gene Sequence: >gb|AY116918|ATCC 13048|Enterobacter aerogenes strain ATCC 13048 23S ribosomal RNA gene,partial sequence.| ggttaagcgactaag...
Miscellaneous Sequence Data: >gb|AJ300554|CIP 60.86T| ATCC 13048T|Enterobacter aerogenes partial gyrB gene for DNA gyrase B subunitstrain CIP 60.86T, ATCC 13048T.| ataaatttgatgata...
Bibliography: HORMAECHE E & EDWARDS P R 1960 INT BULL BACT NOMEN CL TAXON 10 71;
Extended Bibliography: showhide Show bibliography
Ref #: 95525
Author(s): Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F.
Journal: Br J Biomed Sci
Title: Problems in identifying blood culture isolates
Volume: 55
Page(s): 12-3
Year: 1998
Keyword(s): GENBANK/AJ001236 GENBANK/AJ001237 GENBANK/AJ001238 GENBANK/AJ001239 GENBANK/AJ001240 GENBANK/AJ001241 GENBANK/AJ001242 GENBANK/AJ001243 GENBANK/AJ001244 GENBANK/AJ001245 Adolescent *Bacterial Typing Techniques Blood/*microbiology Female Gram-Negative Bacteria/*classification Humans Phylogeny RNA, Bacterial/genetics Sequence Analysis, RNA
URL: 9684412
Ref #: 95492
Author(s): Fukushima,M.;Kakinuma,K.;Kawaguchi,R.
Journal: J Clin Microbiol
Title: Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli strains on the basis of the gyrB gene sequence
Volume: 40
Page(s): 2779-85
Year: 2002
Keyword(s): DNA Gyrase/*genetics DNA, Bacterial/analysis DNA, Ribosomal/analysis Escherichia coli/classification/*genetics Humans Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Salmonella/classification/*genetics Shigella/classification/*genetics
Remarks: Phylogenetic analysis of about 200 strains of Salmonella, Shigella, and Escherichia coli was carried out using the nucleotide sequence of the gene for DNA gyrase B (gyrB), which was determined by directly sequencing PCR fragments. The results establish a new phylogenetic tree for the classification of Salmonella, Shigella, and Escherichia coli in which Salmonella forms a cluster separate from but closely related to Shigella and E. coli. In comparison with 16S rRNA analysis, the gyrB sequences indicated a greater evolutionary divergence for the bacteria. Thus, in screening for the presence of bacteria, the gyrB gene might be a useful tool for differentiating between closely related species of bacteria such as Shigella spp. and E. coli. At present, 16S rRNA sequence analysis is an accurate and rapid method for identifying most unknown bacteria to the genus level because the highly conserved 16S rRNA region is easy to amplify; however, analysis of the more variable gyrB sequence region can identify unknown bacteria to the species level. In summary, we have shown that gyrB sequence analysis is a useful alternative to 16S rRNA analysis for constructing the phylogenetic relationships of bacteria, in particular for the classification of closely related bacterial species.
URL: 12149329
Ref #: 48709
Author(s): Dauga,C.
Journal: Int J Syst Evol Microbiol
Title: Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies
Volume: 52
Page(s): 531-47
Year: 2002
Keyword(s): GENBANK/AJ300528 GENBANK/AJ300529 GENBANK/AJ300530 GENBANK/AJ300531 GENBANK/AJ300532 GENBANK/AJ300533 GENBANK/AJ300534 GENBANK/AJ300535 GENBANK/AJ300536 GENBANK/AJ300537 GENBANK/AJ300538 GENBANK/AJ300539 GENBANK/AJ300540 GENBANK/AJ300541 GENBANK/AJ300542 GENBANK/AJ300543 GENBANK/AJ300544 GENBANK/AJ300545 GENBANK/AJ300546 GENBANK/AJ300547 GENBANK/AJ300548 GENBANK/AJ300549 GENBANK/AJ300550 GENBANK/AJ300551 GENBANK/AJ300552 GENBANK/AJ300553 GENBANK/AJ300554 DNA Gyrase/*genetics Enterobacteriaceae/*classification/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Phenotype RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry
Remarks: Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships.
URL: 11931166
Ref #: 12648
Author(s): Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F.
Journal: Br J Biomed Sci
Title: Problems in identifying blood culture isolates
Volume: 55
Page(s): 12-3
Year: 1998
Keyword(s): GENBANK/AJ001236 GENBANK/AJ001237 GENBANK/AJ001238 GENBANK/AJ001239 GENBANK/AJ001240 GENBANK/AJ001241 GENBANK/AJ001242 GENBANK/AJ001243 GENBANK/AJ001244 GENBANK/AJ001245 Adolescent *Bacterial Typing Techniques Blood/*microbiology Case Report Female Gram-Negative Bacteria/*classification Human Phylogeny RNA, Bacterial/genetics Sequence Analysis, RNA Support, Non-U.S. Gov't
URL: 98349037
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 4300
Author(s): Jagnow,G.;Haider,K.;Ellwardt,P.-C.
Journal: Arch. Microbiol.
Title: Anaerobic dechlorination and degradation of hexachlorocyclohexane isomers by anaerobic and facultative anaerobic bacteria.
Volume: 115
Page(s): 285-292
Year: 1977
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (Cloaca B, ATCC 13048) Type strain / P. R. Edwards, CDC Atlanta in 1958 / Sputum / Hormaeche, E. & Edwards, P. R. (1958) Int. Bull. bact. Nomencl. Taxon. 8, 111 and (1960) 10, 71
Accession Date: 01/01/1958
History: EDWARDS P R CDC ATLANTA
Authority: Hormaeche and Edwards 1960, comb. nov.
Depositor: EDWARDS P R
Taxonomy: TaxLink: S1128 (Enterobacter aerogenes Hormaeche and Edwards 1960) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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