Culture Collections

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Bacteria Collection: Pasteurella multocida

NCTC Number: NCTC 10322
Current Name: Pasteurella multocida
Original Strain Reference: W-9217
Other Collection No: ATCC 43137; DSM 16031
Previous Catalogue Name: Pasteurella multocida
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar carters group a
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, porcine
16S rRNA Gene Sequence: >gb|AY078999|NCTC 10322|Pasteurella multocida subsp. multocida strain NCTC 10322 16Sribosomal RNA gene, partial sequence.| tcagattgaacgctg... >gb|AF176289|NCTC10322|Pasteurella multocida strain NCTC10322 16S ribosomal RNA gene,partial sequence.| ggttaagctatctac...
Bibliography: CARTER G R 1963 VET REC 75 1264;CARTER G R 1963 VET REC 75 1264
Extended Bibliography: showhide Show bibliography
Ref #: 67565
Author(s): Liu,S.L.;Schryvers,A.B.;Sanderson,K.E.;Johnston,R.N.
Journal: J Bacteriol
Title: Bacterial phylogenetic clusters revealed by genome structure
Volume: 181
Page(s): 6747-55
Year: 1999
Keyword(s): GENBANK/AF176279 GENBANK/AF176280 GENBANK/AF176281 GENBANK/AF176282 GENBANK/AF176283 GENBANK/AF176284 GENBANK/AF176285 GENBANK/AF176286 GENBANK/AF176287 GENBANK/AF176288 GENBANK/AF176289 GENBANK/AF176290 GENBANK/AF176291 GENBANK/AF176292 GENBANK/AF176293 GENBANK/AF176294 GENBANK/AF176295 GENBANK/AF176296 GENBANK/AF176297 GENBANK/AF176298 GENBANK/AF176299 GENBANK/AF176300 GENBANK/AF176301 Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/genetics/metabolism Electrophoresis, Gel, Pulsed-Field Endodeoxyribonucleases/metabolism *Genome, Bacterial Molecular Sequence Data Pasteurella/classification/*genetics *Phylogeny RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Salmonella/classification/*genetics
Remarks: Current bacterial taxonomy is mostly based on phenotypic criteria, which may yield misleading interpretations in classification and identification. As a result, bacteria not closely related may be grouped together as a genus or species. For pathogenic bacteria, incorrect classification or misidentification could be disastrous. There is therefore an urgent need for appropriate methodologies to classify bacteria according to phylogeny and corresponding new approaches that permit their rapid and accurate identification. For this purpose, we have devised a strategy enabling us to resolve phylogenetic clusters of bacteria by comparing their genome structures. These structures were revealed by cleaving genomic DNA with the endonuclease I-CeuI, which cuts within the 23S ribosomal DNA (rDNA) sequences, and by mapping the resulting large DNA fragments with pulsed-field gel electrophoresis. We tested this experimental system on two representative bacterial genera: Salmonella and Pasteurella. Among Salmonella spp., I-CeuI mapping revealed virtually indistinguishable genome structures, demonstrating a high degree of structural conservation. Consistent with this, 16S rDNA sequences are also highly conserved among the Salmonella spp. In marked contrast, the Pasteurella strains have very different genome structures among and even within individual species. The divergence of Pasteurella was also reflected in 16S rDNA sequences and far exceeded that seen between Escherichia and Salmonella. Based on this diversity, the Pasteurella haemolytica strains we analyzed could be divided into 14 phylogenetic groups and the Pasteurella multocida strains could be divided into 9 groups. If criteria for defining bacterial species or genera similar to those used for Salmonella and Escherichia coli were applied, the striking phylogenetic diversity would allow bacteria in the currently recognized species of P. multocida and P. haemolytica to be divided into different species, genera, or even higher ranks. On the other hand, strains of Pasteurella ureae and Pasteurella pneumotropica are very similar to those of P. multocida in both genome structure and 16S rDNA sequence and should be regarded as strains within this species. We conclude that large-scale genome structure can be a sensitive indicator of phylogenetic relationships and that, therefore, I-CeuI-based genomic mapping is an efficient tool for probing the phylogenetic status of bacteria.
URL: 10542177
Ref #: 67387
Author(s): Petersen,K.D.;Christensen,H.;Bisgaard,M.;Olsen,J.E.
Journal: Microbiology
Title: Genetic diversity of Pasteurella multocida fowl cholera isolates as demonstrated by ribotyping and 16S rRNA and partial atpD sequence comparisons
Volume: 147
Page(s): 2739-48
Year: 2001
Keyword(s): GENBANK/AF326323 GENBANK/AF326324 GENBANK/AF326325 Animals Bacterial Proton-Translocating ATPases/genetics Bird Diseases/*microbiology Birds DNA, Ribosomal/analysis/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Pasteurella Infections/microbiology/*veterinary Pasteurella multocida/*classification/*genetics Phylogeny RNA, Ribosomal, 16S/genetics Ribotyping Sequence Analysis, DNA *Variation (Genetics)
Remarks: The genetic diversity of Pasteurella multocida, the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of P. multocida were also included. Avian isolates of P. multocida subsp. multocida and P. multocida subsp. septica did not represent separate lines by HpaII ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of P. multocida subspp. multocida and septica. By ribotyping, all P. multocida subsp. gallicida strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of P. multocida subsp. gallicida are genetically related and may be distantly related to other P. multocida isolates, including those of avian origin. By 16S rRNA and atpD sequence comparisons of selected strains, including both P. multocida isolated from birds and mammals and selected distantly related Pasteurella species associated with birds and mammals, it was found that P. multocida is monophyletic. Extended DNA-DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of P. multocida fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.
URL: 11577153
Ref #: 67383
Author(s): Davies,R.L.
Journal: Microbiology
Title: Genetic diversity among Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of the 16S rRNA gene
Volume: 150
Page(s): 4199-210
Year: 2004
Keyword(s): GENBANK/AY078996 GENBANK/AY078998 GENBANK/AY078999 GENBANK/AY079000 GENBANK/AY299304 GENBANK/AY299305 GENBANK/AY299306 GENBANK/AY299307 GENBANK/AY299308 GENBANK/AY299309 GENBANK/AY299310 GENBANK/AY299311 GENBANK/AY299312 GENBANK/AY299313 GENBANK/AY299314 GENBANK/AY299315 GENBANK/AY299316 GENBANK/AY299317 GENBANK/AY299318 GENBANK/AY299319 GENBANK/AY324032 Animals Base Sequence Cats Cattle/microbiology England Galactitol/metabolism Genes, rRNA Molecular Sequence Data Pasteurella Infections/microbiology/+ACo-veterinary Pasteurella multocida/+ACo-classification/genetics/metabolism Phylogeny Poultry/microbiology RNA, Ribosomal, 16S/+ACo-genetics Sequence Analysis, DNA Sheep/microbiology Sorbitol/metabolism Swine/microbiology +ACo-Variation (Genetics) Wales
Remarks: Genetic diversity among 86 Pasteurella multocida isolates was investigated by comparative sequence analysis of a 1468 bp fragment of the 16S rRNA gene. The strains included 79 field isolates recovered from birds (poultry) (22), cattle (21), pigs (26) and sheep (10) within England and Wales, four Asian isolates associated with bovine haemorrhagic septicaemia, and the type strains of the three subspecies of P. multocida. Dulcitol and sorbitol fermentation patterns were also determined to establish correlations between subspecies status and phylogenetic relatedness. Nineteen 16S rRNA types were identified, but these were clustered into two distinct phylogenetic lineages, A and B. Sequences within lineages A and B had a mean number of nucleotide differences of 21.123.90. Isolates within lineage A were associated with birds, cattle, pigs and sheep, whereas those belonging to lineage B were recovered from birds and a cat. Eighty-seven per cent of the isolates were classified as P. multocida subsp. multocida by dulcitol and sorbitol fermentation patterns, but these have diverse 16S rRNA gene sequences that were represented in both lineages A and B. Avian P. multocida subsp. septica isolates were associated exclusively with lineage B, but bovine P. multocida subsp. septica isolates were present in lineage A. P. multocida subsp. gallicida isolates of avian, bovine and porcine origin represent a homogeneous group within lineage A, but they have the same 16S rRNA type as certain P. multocida subsp. multocida isolates. These findings provide strong support for the view that dulcitol and sorbitol fermentation patterns are inaccurate indicators of genetic relatedness among P. multocida strains. Avian capsular type B isolates and capsular type B and E isolates associated with haemorrhagic septicaemia of cattle and water buffaloes are closely related and form a distinct cluster within lineage A. The current subspecies nomenclature of P. multocida neither accurately reflects the 16S rRNA-based phylogenetic relationships among isolates nor does it adequately encompass the full range of diversity within the species. The study provides a 16S rRNA-based evolutionary framework that will form the basis of further studies into the genetic diversity of P. multocida and will also help in the reclassification of the species.
URL: 15583172
Ref #: 13710
Author(s): Liu,S.L.;Schryvers,A.B.;Sanderson,K.E.;Johnston,R.N.
Journal: J Bacteriol
Title: Bacterial phylogenetic clusters revealed by genome structure
Volume: 181
Page(s): 6747-55
Year: 1999
Keyword(s): GENBANK/AF176279 GENBANK/AF176280 GENBANK/AF176281 GENBANK/AF176282 GENBANK/AF176283 GENBANK/AF176284 GENBANK/AF176285 GENBANK/AF176286 GENBANK/AF176287 GENBANK/AF176288 GENBANK/AF176289 GENBANK/AF176290 GENBANK/AF176291 GENBANK/AF176292 GENBANK/AF176293 GENBANK/AF176294 GENBANK/AF176295 GENBANK/AF176296 GENBANK/AF176297 GENBANK/AF176298 GENBANK/AF176299 GENBANK/AF176300 GENBANK/AF176301 Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/genetics/metabolism Electrophoresis, Gel, Pulsed-Field Endodeoxyribonucleases/metabolism *Genome, Bacterial Molecular Sequence Data Pasteurella/classification/*genetics *Phylogeny RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Salmonella/classification/*genetics Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Remarks: Current bacterial taxonomy is mostly based on phenotypic criteria, which may yield misleading interpretations in classification and identification. As a result, bacteria not closely related may be grouped together as a genus or species. For pathogenic bacteria, incorrect classification or misidentification could be disastrous. There is therefore an urgent need for appropriate methodologies to classify bacteria according to phylogeny and corresponding new approaches that permit their rapid and accurate identification. For this purpose, we have devised a strategy enabling us to resolve phylogenetic clusters of bacteria by comparing their genome structures. These structures were revealed by cleaving genomic DNA with the endonuclease I-CeuI, which cuts within the 23S ribosomal DNA (rDNA) sequences, and by mapping the resulting large DNA fragments with pulsed-field gel electrophoresis. We tested this experimental system on two representative bacterial genera: Salmonella and Pasteurella. Among Salmonella spp., I-CeuI mapping revealed virtually indistinguishable genome structures, demonstrating a high degree of structural conservation. Consistent with this, 16S rDNA sequences are also highly conserved among the Salmonella spp. In marked contrast, the Pasteurella strains have very different genome structures among and even within individual species. The divergence of Pasteurella was also reflected in 16S rDNA sequences and far exceeded that seen between Escherichia and Salmonella. Based on this diversity, the Pasteurella haemolytica strains we analyzed could be divided into 14 phylogenetic groups and the Pasteurella multocida strains could be divided into 9 groups. If criteria for defining bacterial species or genera similar to those used for Salmonella and Escherichia coli were applied, the striking phylogenetic diversity would allow bacteria in the currently recognized species of P. multocida and P. haemolytica to be divided into different species, genera, or even higher ranks. On the other hand, strains of Pasteurella ureae and Pasteurella pneumotropica are very similar to those of P. multocida in both genome structure and 16S rDNA sequence and should be regarded as strains within this species. We conclude that large-scale genome structure can be a sensitive indicator of phylogenetic relationships and that, therefore, I-CeuI-based genomic mapping is an efficient tool for probing the phylogenetic status of bacteria.
URL: 20011342
Ref #: 13368
Author(s): Petersen,K.D.;Christensen,H.;Bisgaard,M.;Olsen,J.E.
Journal: Microbiology
Title: Genetic diversity of Pasteurella multocida fowl cholera isolates as demonstrated by ribotyping and 16S rRNA and partial atpD sequence comparisons
Volume: 147
Page(s): 2739-48
Year: 2001
Keyword(s): GENBANK/AF326323 GENBANK/AF326324 GENBANK/AF326325 Animal Bacterial Proton-Translocating ATPases/genetics Bird Diseases/*microbiology Birds Comparative Study DNA, Ribosomal/analysis/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Pasteurella Infections/microbiology/*veterinary Pasteurella multocida/*classification/*genetics Phylogeny RNA, Ribosomal, 16S/genetics Ribotyping Sequence Analysis, DNA Support, Non-U.S. Gov't *Variation (Genetics)
Remarks: The genetic diversity of Pasteurella multocida, the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of P. multocida were also included. Avian isolates of P. multocida subsp. multocida and P. multocida subsp. septica did not represent separate lines by HpaII ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of P. multocida subspp. multocida and septica. By ribotyping, all P. multocida subsp. gallicida strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of P. multocida subsp. gallicida are genetically related and may be distantly related to other P. multocida isolates, including those of avian origin. By 16S rRNA and atpD sequence comparisons of selected strains, including both P. multocida isolated from birds and mammals and selected distantly related Pasteurella species associated with birds and mammals, it was found that P. multocida is monophyletic. Extended DNA-DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of P. multocida fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.
URL: 21461099
Data: Type strain of ss multocida (ATCC 43137) G. R. Carter, Quebec in 1962 / Porcine / Carter, G. R. (1963) Vet. Rec. 75, 1264 / Subsp. Multocida
Accession Date: 01/01/1962
History: CARTER G R, ANIMAL DIS RES INST, HULL, QEUBEC, CANADA
Authority: (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939 (AL)
Depositor: CARTER G R
Taxonomy: TaxLink: S2224 (Pasteurella multocida subsp. multocida (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939) - Date of change: 5/02/2003
Other: Carters group A
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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