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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Fusobacterium nucleatum subsp. polymorphum

NCTC Number: NCTC 10562
Current Name: Fusobacterium nucleatum subsp. polymorphum
Original Strain Reference: 555A
Other Collection No: ATCC 10953; CCUG 9126; DSM 20482; JCM 12990
Previous Catalogue Name: Fusobacterium nucleatum subsp. polymorphum
Other Names: FUSOBACTERIUM POLYMORPHUM
Type Strain: Yes
Family: Fusobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: viande-leure broth,37, strict anaerobe
Isolated From: human, inflamed gingiva
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/SAMEA2479572
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF342832|ATCC 10953|Fusobacterium nucleatum subsp. polymorphum strain ATCC 10953 16Sribosomal RNA gene, partial sequence; internal transcribed spacer1, complete sequence; and 23S ribosomal RNA gene, partial sequence.| gaagtagcaggccta... >gb|AF287812|ATCC 10953|Fusobacterium nucleatum subsp. polymorphum 16S ribosomal RNA gene,partial sequence.| tgaacgaagagtttg...
23S rRNA Gene Sequence: >gb|AJ307974|DSM 20482|Fusobacterium nucleatum 23S rRNA gene, strain DSM 20482.| taggttaaaataatt... >gb|AF342832|ATCC 10953|Fusobacterium nucleatum subsp. polymorphum strain ATCC 10953 16Sribosomal RNA gene, partial sequence; internal transcribed spacer1, complete sequence; and 23S ribosomal RNA gene, partial sequence.| gaagtagcaggccta...
Bibliography: HOFFMANN H 1955 OHIO J SCI 53 141;HOFFMANN H 1951 J BACT 61 241
Extended Bibliography: showhide Show bibliography
Ref #: 46426
Author(s): Conrads,G.;Claros,M.C.;Citron,D.M.;Tyrrell,K.L.;Merriam,V.;Goldstein,E.J.
Journal: Int J Syst Evol Microbiol
Title: 16S-23S rDNA internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Fusobacterium
Volume: 52
Page(s): 493-9
Year: 2002
Keyword(s): GENBANK/AF342829 GENBANK/AF342830 GENBANK/AF342831 GENBANK/AF342832 GENBANK/AF342833 GENBANK/AF342834 GENBANK/AF342835 GENBANK/AF342836 GENBANK/AF342837 GENBANK/AF342838 GENBANK/AF342839 GENBANK/AF342840 GENBANK/AF342841 GENBANK/AF342842 GENBANK/AF342843 GENBANK/AF342844 GENBANK/AF342845 GENBANK/AF342846 GENBANK/AF342847 GENBANK/AF342848 GENBANK/AF342849 GENBANK/AF342850 GENBANK/AF342851 GENBANK/AF342852 GENBANK/AF342853 GENBANK/AF342854 GENBANK/AF342855 GENBANK/AF342856 GENBANK/AF342857 GENBANK/AF342858 GENBANK/AF342859 GENBANK/AF342860 GENBANK/AF342861 Base Sequence DNA Primers DNA, Ribosomal Spacer/*genetics Fusobacterium/*classification/genetics Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry Species Specificity
Remarks: The 16S-23S rDNA internal transcribed spacer (ITS) regions of all currently defined Fusobacterium species and related taxa such as Leptotrichia buccalis, Sebaldella termitidis and Streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. For the ITS-amplification, a new eubacterial universal primer pair was designed and used. The majority of the Fusobacterium strains, along with L. buccalis showed one major, and two to three weaker, distinct bands (short and long versions) with lengths of 800-830 bp and 1000-1100 bp. Nevertheless, six other patterns were also found within the genus Fusobacterium, demonstrating its heterogeneity. The ITS region was sequenced and found to consist both of conserved motifs, which functioned as a framework for alignment, and of variable sites, which provided high phylogenetic resolution. Analyses of the ITS-DNA sequences and ITS relative length (short version) allowed species and subspecies differentiation in most cases. The results confirmed the strikingly distant relationship between Fusobacterium prausnitzii and the genus Fusobacterium. Fusobacterium nucleatum subspecies, along with Fusobacterium naviforme, Fusobacterium simiae and Fusobacterium periodonticum, formed a cluster with an inherently high potential for diversification. Other clusters were formed by Fusobacterium necrophorum subspecies with Fusobacterium gonidaformans and by Fusobacterium varium with Fusobacterium mortiferum and Fusobacterium ulcerans. Fusobacterium russii as well as Fusobacterium perfoetens formed separate branches. Fusobacterium necrophorum subspp. necrophorum and funduliforme on the one hand, and Fusobacterium varium and Fusobacterium mortiferum on the other, were found to be very similar, even at the high-resolution ITS level.
URL: 11931161
Ref #: 50644
Author(s): Brune,A.;Evers,S.;Kaim,G.;Ludwig,W.;Schink,B.
Journal: Int J Syst Evol Microbiol
Title: Ilyobacter insuetus sp. nov., a fermentative bacterium specialized in the degradation of hydroaromatic compounds
Volume: 52
Page(s): 429-32
Year: 2002
Keyword(s): GENBANK/AJ307976 GENBANK/AJ307980 Biodegradation, Environmental DNA, Bacterial/chemistry DNA, Ribosomal/chemistry Fermentation Gram-Negative Aerobic Rods and Cocci/*classification/genetics/metabolism Molecular Sequence Data Phylogeny Quinic Acid/metabolism RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry Sequence Analysis, DNA Shikimic Acid/metabolism
Remarks: The mesophilic, anaerobic bacterium strain VenChi2T was isolated with quinic acid (1,3,4,5-tetrahydroxy-cyclohexane-1-carboxylic acid) as the sole source of carbon and energy. Of more than 30 substrates tested, only quinic acid and shikimic acid (3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid) were utilized, yielding acetate, propionate, butyrate, H2 and CO2 as fermentation products. Sugars, alcohols, (di-)carboxylic acids, amino acids and aromatic compounds were not fermented and no external electron acceptors were used. Strain VenChi2T is a gram-negative, strictly anaerobic, coccoid rod; it does not employ the classical hydroaromatic pathway of aerobic bacteria for the degradation of hydroaromatic compounds (no aromatic intermediates involved). Comparative 16S and 23S rDNA sequence analyses placed strain VenChi2T in the fusobacteria phylum, with the closest relatives among species of the genera Ilyobacter and Propionigenium. The results indicate that, disregarding the taxonomically misplaced Ilyobacter delafieldii, which is a member of the clostridia, the validly described Ilyobacter and Propionigenium species are phylogenetically intermixed. Based on its phenotypic properties, strain VenChi2T (= DSM 6831T = ATCC BAA-291T) is assigned to the genus Ilyobacter as the type strain of a novel species, Ilyobacter insuetus sp. nov.
URL: 11931152
Ref #: 25413
Author(s): Paster,B.J.;Boches,S.K.;Galvin,J.L.;Ericson,R.E.;Lau,C.N.;Levanos,V.A.;Sahasrabudhe,A.;Dewhirst,F.E.
Journal: J Bacteriol
Title: Bacterial diversity in human subgingival plaque
Volume: 183
Page(s): 3770-83
Year: 2001
Keyword(s): Bacteria/*classification/genetics/isolation & purification DNA, Bacterial/analysis DNA, Ribosomal/analysis Dental Plaque/*microbiology Gingiva/*microbiology Humans Periodontal Diseases/*microbiology Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/analysis Sequence Analysis, DNA
Remarks: The purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species of human oral bacteria. Subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16S ribosomal DNA (rDNA) bacterial genes from DNA isolated from subgingival plaque samples were PCR amplified with all-bacterial or selective primers and cloned into Escherichia coli. The sequences of cloned 16S rDNA inserts were used to determine species identity or closest relatives by comparison with sequences of known species. A total of 2,522 clones were analyzed. Nearly complete sequences of approximately 1,500 bases were obtained for putative new species. About 60% of the clones fell into 132 known species, 70 of which were identified from multiple subjects. About 40% of the clones were novel phylotypes. Of the 215 novel phylotypes, 75 were identified from multiple subjects. Known putative periodontal pathogens such as Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola were identified from multiple subjects, but typically as a minor component of the plaque as seen in cultivable studies. Several phylotypes fell into two recently described phyla previously associated with extreme natural environments, for which there are no cultivable species. A number of species or phylotypes were found only in subjects with disease, and a few were found only in healthy subjects. The organisms identified only from diseased sites deserve further study as potential pathogens. Based on the sequence data in this study, the predominant subgingival microbial community consisted of 347 species or phylotypes that fall into 9 bacterial phyla. Based on the 347 species seen in our sample of 2,522 clones, we estimate that there are 68 additional unseen species, for a total estimate of 415 species in the subgingival plaque. When organisms found on other oral surfaces such as the cheek, tongue, and teeth are added to this number, the best estimate of the total species diversity in the oral cavity is approximately 500 species, as previously proposed.
URL: 11371542
Ref #: 12738
Author(s): Paster,B.J.;Boches,S.K.;Galvin,J.L.;Ericson,R.E.;Lau,C.N.;Levanos,V.A.;Sahasrabudhe,A.;Dewhirst,F.E.
Journal: J Bacteriol
Title: Bacterial diversity in human subgingival plaque
Volume: 183
Page(s): 3770-83
Year: 2001
Keyword(s): Bacteria/*classification/genetics/isolation & purification DNA, Bacterial/analysis DNA, Ribosomal/analysis Dental Plaque/*microbiology Gingiva/*microbiology Human Periodontal Diseases/*microbiology Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/analysis Sequence Analysis, DNA Support, U.S. Gov't, P.H.S.
Remarks: The purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species of human oral bacteria. Subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16S ribosomal DNA (rDNA) bacterial genes from DNA isolated from subgingival plaque samples were PCR amplified with all-bacterial or selective primers and cloned into Escherichia coli. The sequences of cloned 16S rDNA inserts were used to determine species identity or closest relatives by comparison with sequences of known species. A total of 2,522 clones were analyzed. Nearly complete sequences of approximately 1,500 bases were obtained for putative new species. About 60% of the clones fell into 132 known species, 70 of which were identified from multiple subjects. About 40% of the clones were novel phylotypes. Of the 215 novel phylotypes, 75 were identified from multiple subjects. Known putative periodontal pathogens such as Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola were identified from multiple subjects, but typically as a minor component of the plaque as seen in cultivable studies. Several phylotypes fell into two recently described phyla previously associated with extreme natural environments, for which there are no cultivable species. A number of species or phylotypes were found only in subjects with disease, and a few were found only in healthy subjects. The organisms identified only from diseased sites deserve further study as potential pathogens. Based on the sequence data in this study, the predominant subgingival microbial community consisted of 347 species or phylotypes that fall into 9 bacterial phyla. Based on the 347 species seen in our sample of 2,522 clones, we estimate that there are 68 additional unseen species, for a total estimate of 415 species in the subgingival plaque. When organisms found on other oral surfaces such as the cheek, tongue, and teeth are added to this number, the best estimate of the total species diversity in the oral cavity is approximately 500 species, as previously proposed.
URL: 21264390
Ref #: 12703
Author(s): Conrads,G.;Claros,M.C.;Citron,D.M.;Tyrrell,K.L.;Merriam,V.;Goldstein,E.J.
Journal: Int J Syst Evol Microbiol
Title: 16S-23S rDNA internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Fusobacterium
Volume: 52
Page(s): 493-9
Year: 2002
Keyword(s): GENBANK/AF342829 GENBANK/AF342830 GENBANK/AF342831 GENBANK/AF342832 GENBANK/AF342833 GENBANK/AF342834 GENBANK/AF342835 GENBANK/AF342836 GENBANK/AF342837 GENBANK/AF342838 GENBANK/AF342839 GENBANK/AF342840 GENBANK/AF342841 GENBANK/AF342842 GENBANK/AF342843 GENBANK/AF342844 GENBANK/AF342845 GENBANK/AF342846 GENBANK/AF342847 GENBANK/AF342848 GENBANK/AF342849 GENBANK/AF342850 GENBANK/AF342851 GENBANK/AF342852 GENBANK/AF342853 GENBANK/AF342854 GENBANK/AF342855 GENBANK/AF342856 GENBANK/AF342857 GENBANK/AF342858 GENBANK/AF342859 GENBANK/AF342860 GENBANK/AF342861 Base Sequence DNA Primers DNA, Ribosomal Spacer/*genetics Fusobacterium/*classification/genetics Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry Species Specificity Support, Non-U.S. Gov't
Remarks: The 16S-23S rDNA internal transcribed spacer (ITS) regions of all currently defined Fusobacterium species and related taxa such as Leptotrichia buccalis, Sebaldella termitidis and Streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. For the ITS-amplification, a new eubacterial universal primer pair was designed and used. The majority of the Fusobacterium strains, along with L. buccalis showed one major, and two to three weaker, distinct bands (short and long versions) with lengths of 800-830 bp and 1000-1100 bp. Nevertheless, six other patterns were also found within the genus Fusobacterium, demonstrating its heterogeneity. The ITS region was sequenced and found to consist both of conserved motifs, which functioned as a framework for alignment, and of variable sites, which provided high phylogenetic resolution. Analyses of the ITS-DNA sequences and ITS relative length (short version) allowed species and subspecies differentiation in most cases. The results confirmed the strikingly distant relationship between Fusobacterium prausnitzii and the genus Fusobacterium. Fusobacterium nucleatum subspecies, along with Fusobacterium naviforme, Fusobacterium simiae and Fusobacterium periodonticum, formed a cluster with an inherently high potential for diversification. Other clusters were formed by Fusobacterium necrophorum subspecies with Fusobacterium gonidaformans and by Fusobacterium varium with Fusobacterium mortiferum and Fusobacterium ulcerans. Fusobacterium russii as well as Fusobacterium perfoetens formed separate branches. Fusobacterium necrophorum subspp. necrophorum and funduliforme on the one hand, and Fusobacterium varium and Fusobacterium mortiferum on the other, were found to be very similar, even at the high-resolution ITS level.
URL: 21928123
Ref #: 12904
Author(s): Brune,A.;Evers,S.;Kaim,G.;Ludwig,W.;Schink,B.
Journal: Int J Syst Evol Microbiol
Title: Ilyobacter insuetus sp. nov., a fermentative bacterium specialized in the degradation of hydroaromatic compounds
Volume: 52
Page(s): 429-32
Year: 2002
Keyword(s): GENBANK/AJ307976 GENBANK/AJ307980 Biodegradation DNA, Bacterial/chemistry DNA, Ribosomal/chemistry Fermentation Gram-Negative Aerobic Rods and Cocci/*classification/genetics/metabolism Molecular Sequence Data Phylogeny Quinic Acid/metabolism RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry Sequence Analysis, DNA Shikimic Acid/metabolism Support, Non-U.S. Gov't
Remarks: The mesophilic, anaerobic bacterium strain VenChi2T was isolated with quinic acid (1,3,4,5-tetrahydroxy-cyclohexane-1-carboxylic acid) as the sole source of carbon and energy. Of more than 30 substrates tested, only quinic acid and shikimic acid (3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid) were utilized, yielding acetate, propionate, butyrate, H2 and CO2 as fermentation products. Sugars, alcohols, (di-)carboxylic acids, amino acids and aromatic compounds were not fermented and no external electron acceptors were used. Strain VenChi2T is a gram-negative, strictly anaerobic, coccoid rod; it does not employ the classical hydroaromatic pathway of aerobic bacteria for the degradation of hydroaromatic compounds (no aromatic intermediates involved). Comparative 16S and 23S rDNA sequence analyses placed strain VenChi2T in the fusobacteria phylum, with the closest relatives among species of the genera Ilyobacter and Propionigenium. The results indicate that, disregarding the taxonomically misplaced Ilyobacter delafieldii, which is a member of the clostridia, the validly described Ilyobacter and Propionigenium species are phylogenetically intermixed. Based on its phenotypic properties, strain VenChi2T (= DSM 6831T = ATCC BAA-291T) is assigned to the genus Ilyobacter as the type strain of a novel species, Ilyobacter insuetus sp. nov.
URL: 21928114
Ref #: 1355
Author(s): Kato,K.;Umemoto,T.;Sagawa,H.;Kotani,S.
Journal: Curr. Microbiol.
Title: Lanthionine as an essential constituent of cell wall peptidoglycan of Fusobacterium nucleatum.
Volume: 3
Page(s): 147-152
Year: 1979
Ref #: 3893
Author(s): Dzink,J.L.;Sheenan,M.T.;Socransky,S.S.
Journal: Int. J. Syst. Bacteriol.
Title: Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincen
Volume: 40
Page(s): 74-78
Year: 1990
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 10953) Type strain / ATCC in 1968 / H. Hoffman, Ohio, USA in 1951 / Inflamed gingiva / Fusobacterium polymorphum / Hoffman H. (1951) J. Bact. 61, 241 / Hoffman H. (1955) Ohio. J. Sci. 53, 141 / Dzink, J. et al. (1990) Int. J. syst. Bact. 40, 74
Accession Date: 01/01/1968
History: ISOLATED BY HOFFMANN H OHIO USA IN 1951
Authority: Dzink et al. 1990
Depositor: ATCC
Taxonomy: TaxLink: S1334 (Fusobacterium nucleatum subsp. nucleatum Knorr 1922) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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