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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Burkholderia cepacia

NCTC Number: NCTC 10743
Current Name: Burkholderia cepacia
Original Strain Reference: 717
Other Collection No: ATCC 25416; DSM 7288; 717
Previous Catalogue Name: Burkholderia cepacia
Type Strain: Yes
Family: Burkholderiaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: not recorded
16S rRNA Gene Sequence: >gb|AF097530|ATCC25416|Burkholderia cepacia strain ATCC25416 16S ribosomal RNA gene,partial sequence.| agtttgatcctggct... >gb|U96927|ATCC 25416T|Burkholderia cepacia 16S ribosomal RNA gene, partial sequence.| atccaagtcgaacgg... >gb|AY728197|ATCC 25416|Burkholderia cepacia strain ATCC 25416 16S ribosomal RNA gene,partial sequence.| tactgggcgtaaagc... >gb|D13046|ATCC25416| EY 645|Pseudomonas cepacia 16S rRNA, partial sequence.| gggcttcacacgtca...
Bibliography: BURKHOLDER W H 1950 PHYTOPATHOLOGY 40 115; BALLARD R W ET. AL. 1970
Extended Bibliography: showhide Show bibliography
Ref #: 30826
Author(s): Viallard,V.;Poirier,I.;Cournoyer,B.;Haurat,J.;Wiebkin,S.;Ophel-Keller,K.;Balandreau,J.
Journal: Int J Syst Bacteriol
Title: Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia
Volume: 48 Pt 2
Page(s): 549-63
Year: 1998
Keyword(s): GENBANK/U96927 GENBANK/U96928 GENBANK/U96929 GENBANK/U96930 GENBANK/U96931 GENBANK/U96932 GENBANK/U96933 GENBANK/U96934 GENBANK/U96935 GENBANK/U96936 GENBANK/U96937 GENBANK/U96938 GENBANK/U96939 GENBANK/U96940 GENBANK/U96941 Base Sequence Burkholderia/*classification/genetics/metabolism Carbohydrate Metabolism DNA, Bacterial Fatty Acids/analysis Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny Pseudomonas/*classification Triticum/microbiology Zea mays/microbiology
Remarks: In a survey of soil and wheat or maize rhizoplane bacteria isolated using a medium containing azelaic acid and tryptamine as sole carbon and nitrogen sources, respectively, a large proportion of Burkholderia-like bacteria were found. Among them, a homogeneous group of strains was identifiable based on phenotypic properties, fatty acid composition, DNA-DNA hybridizations and 16S rDNA sequences. According to molecular data, this group belongs to the genus Burkholderia but its weak similarity to previously described species suggests that it belongs to a novel species. Closest 16S rDNA phylogenetic neighbours of this species are Burkholderia caryophylli and two previously named Pseudomonas species which clearly appear to be part of the Burkholderia genus and were thus named Burkholderia glathei comb. nov. and Burkholderia phenazinium comb. nov. Strains of the new species are oxidase- and catalase-positive, produce indole and gelatinase, and use L-xylose, lactose, rhamnose, trehalose, D-lyxose, L-arabitol, xylitol and D-raffinose as sole carbon source. This novel taxon is named Burkholderia graminis. In the course of this study, [Pseudomonas] pyrrocinia also proved to be a member of the Burkholderia genus.
URL: 9731297
Ref #: 31060
Author(s): LiPuma,J.J.;Dulaney,B.J.;McMenamin,J.D.;Whitby,P.W.;Stull,T.L.;Coenye,T.;Vandamme,P.
Journal: J Clin Microbiol
Title: Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients
Volume: 37
Page(s): 3167-70
Year: 1999
Keyword(s): GENBANK/AF097530 GENBANK/AF097531 GENBANK/AF097532 GENBANK/AF097533 GENBANK/AF097534 Base Sequence Burkholderia cepacia/classification/*isolation & purification Cystic Fibrosis/*microbiology Humans Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sensitivity and Specificity
Remarks: PCR assays targeting rRNA genes were developed to identify species (genomovars) within the Burkholderia cepacia complex. Each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. These isolates were from clinical and environmental sources and included 107 B. cepacia complex strains, 23 Burkholderia gladioli strains, 20 Ralstonia pickettii strains, 10 Pseudomonas aeruginosa strains, 8 Stenotrophomonas maltophilia strains, and 9 isolates belonging to nine other species. The sensitivity and specificity of the 16S rRNA-based assay for Burkholderia multivorans (genomovar II) were 100 and 99%, respectively; for Burkholderia vietnamiensis (genomovar V), sensitivity and specificity were 87 and 92%, respectively. An assay based on 16S and 23S rRNA gene analysis of B. cepacia ATCC 25416 (genomovar I) was useful in identifying genomovars I, III, and IV as a group (sensitivity, 100%, and specificity, 99%). Another assay, designed to be specific at the genus level, identified all but one of the Burkholderia and Ralstonia isolates tested (sensitivity, 99%, and specificity, 96%). The combined use of these assays offers a significant improvement over previously published PCR assays for B. cepacia.
URL: 10488171
Ref #: 31033
Author(s): Maeda,Y.;Shinohara,H.;Kiba,A.;Ohnishi,K.;Furuya,N.;Kawamura,Y.;Ezaki,T.;Vandamme,P.;Tsushima,S.;Hikichi,Y.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences
Volume: 56
Page(s): 1031-8
Year: 2006
Keyword(s): GENBANK/AB190570 GENBANK/AB190571 GENBANK/AB190572 GENBANK/AB190573 GENBANK/AB190574 GENBANK/AB190575 GENBANK/AB190576 GENBANK/AB190577 GENBANK/AB190578 GENBANK/AB190579 GENBANK/AB190580 GENBANK/AB190581 GENBANK/AB190582 GENBANK/AB190583 GENBANK/AB190584 GENBANK/AB190585 GENBANK/AB190586 GENBANK/AB190587 GENBANK/AB190588 GENBANK/AB190589 GENBANK/AB190590 GENBANK/AB190591 GENBANK/AB190592 GENBANK/AB190593 GENBANK/AB190594 GENBANK/AB190595 GENBANK/AB190596 GENBANK/AB190597 GENBANK/AB190598 GENBANK/AB190599 GENBANK/AB190600 GENBANK/AB190601 GENBANK/AB190602 GENBANK/AB190603 GENBANK/AB190604 GENBANK/AB190605 GENBANK/AB190606 GENBANK/AB190607 GENBANK/AB190608 GENBANK/AB190609 GENBANK/AB190610 GENBANK/AB190611 GENBANK/AB190612 GENBANK/AB190613 GENBANK/AB190614 GENBANK/AB190615 GENBANK/AB190616 GENBANK/AB190617 GENBANK/AB190618 GENBANK/AB190619 GENBANK/AB190620 GENBANK/AB190621 GENBANK/AB190622 GENBANK/AB190623 GENBANK/AB190624 GENBANK/AB190625 GENBANK/AB190626 GENBANK/AB190627 GENBANK/AB190628 GENBANK/AB190629 GENBANK/AB190630 GENBANK/AB190631 GENBANK/AB190632 GENBANK/AB190633 GENBANK/AB190634 GENBANK/AB190635 GENBANK/AB190636 GENBANK/AB190637 GENBANK/AB190638 GENBANK/AB190639 GENBANK/AB190640 GENBANK/AB190641 GENBANK/AB190642 GENBANK/AB190643 GENBANK/AB190644 GENBANK/AB190645 GENBANK/AB190646 GENBANK/AB190647 GENBANK/AB190648 GENBANK/AB190649 GENBANK/AB190650 GENBANK/AB190651 GENBANK/AB190652 GENBANK/AB190653 GENBANK/AB190654 GENBANK/AB190655 GENBANK/AB190656 GENBANK/AB190657 GENBANK/AB190658 GENBANK/AB190659 GENBANK/AB190660 GENBANK/AB190661 GENBANK/AB190662 GENBANK/AB190663 GENBANK/AB190664 GENBANK/AB190665 GENBANK/AB190666 GENBANK/AB190667 GENBANK/AB190668 GENBANK/AB190669 GENBANK/AB190670 GENBANK/AB190671 GENBANK/AB190672 GENBANK/AB190673 GENBANK/AB190674 GENBANK/AB190675 GENBANK/AB190676 GENBANK/AB190677 GENBANK/AB190678 GENBANK/AB190679 GENBANK/AB190680 GENBANK/AB190681 GENBANK/AB190682 GENBANK/AB190683 GENBANK/AB190684 GENBANK/AB190685 GENBANK/AB190686 GENBANK/AB190687 GENBANK/AB190688 GENBANK/AB190689 GENBANK/AB190690 GENBANK/AB190691 GENBANK/AB190692 GENBANK/AB190693 GENBANK/AB190694 GENBANK/AB190695 GENBANK/AB190696 GENBANK/AB190697 GENBANK/AB190698 GENBANK/AB190699 GENBANK/AB190700 GENBANK/AB190701 GENBANK/AB190702 GENBANK/AB190703 GENBANK/AB190704 GENBANK/AB190705 GENBANK/AB190706 GENBANK/AB190707 GENBANK/AB190708 GENBANK/AB190709 GENBANK/AB190710 GENBANK/AB190711 GENBANK/AB190712 GENBANK/AB190713 GENBANK/AB190714 GENBANK/AB190715 GENBANK/AB190716 GENBANK/AB190717 GENBANK/AB190718 GENBANK/AB190719 GENBANK/AB190720 GENBANK/AB190721 GENBANK/AB190722 GENBANK/AB190723 GENBANK/AB190724 GENBANK/AB190725 GENBANK/AB190726 GENBANK/AB190727 GENBANK/AB190728 GENBANK/AB190729 GENBANK/AB190730 GENBANK/AB190731 GENBANK/AB190732 GENBANK/AB190733 GENBANK/AB190734 GENBANK/AB190735 GENBANK/AB190736 GENBANK/AB190737 GENBANK/AB190738 GENBANK/AB190739 GENBANK/AB190740 GENBANK/AB190741 GENBANK/AB190742 GENBANK/AB190743 GENBANK/AB190744 GENBANK/AB190745 GENBANK/AB190746 GENBANK/AB190747 GENBANK/AB190748 GENBANK/AB190749 GENBANK/AB190750 GENBANK/AB190751 GENBANK/AB190752 GENBANK/AB190753 GENBANK/AB190754 GENBANK/AB190755 GENBANK/AB190756 GENBANK/AB190757 GENBANK/AB190758 GENBANK/AB190759 GENBANK/AB190760 GENBANK/AB190761 GENBANK/AB190762 GENBANK/AB190763 GENBANK/AB220897 GENBANK/AB220900 GENBANK/AB220901 GENBANK/AB220902 GENBANK/AB220903 GENBANK/AB220904 GENBANK/AB220908 GENBANK/AB220911 GENBANK/AB220912 GENBANK/AB220913 GENBANK/AB220914 GENBANK/AB239176 GENBANK/AB239177 GENBANK/AB239178 GENBANK/AB239179 GENBANK/AB239180 GENBANK/AB239181 GENBANK/AB239182 GENBANK/AB239183 GENBANK/AB239184 GENBANK/AB239185 GENBANK/AB239186 GENBANK/AB239187 Bacterial Proteins/genetics Burkholderia/classification/*genetics/*isolation & purification DNA Gyrase/*genetics DNA Primers DNA, Bacterial/*analysis/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data Oryza sativa/microbiology Phylogeny Polymerase Chain Reaction Seeds/microbiology Sensitivity and Specificity Sequence Analysis, DNA Sigma Factor/*genetics
Remarks: In order to develop a detection method for the rice pathogens Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli, the phylogeny of six plant-pathogenic Burkholderia species was analysed using the combined nucleotide sequences of gyrB and rpoD. B. plantarii, B. glumae and B. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. The high sequence similarity revealed a close phylogenetic relationship between B. glumae and B. plantarii. B. plantarii strains were divided into three subclusters comprising rice strains, whereas the single Vanda strain occupied a unique position in the phylogenetic tree. The gyrB and rpoD sequences of all B. glumae strains examined were highly conserved. In contrast, B. gladioli strains demonstrated a far greater sequence diversity, but this diversity did not correlate with pathovar, host plant or geographical origin of the strains. A multiplex-PCR protocol using specific primers from the gyrB sequences was designed that allowed the specific detection and identification of B. plantarii, B. glumae and B. gladioli in rice seeds infected with these pathogenic species.
URL: 16627650
Ref #: 12328
Author(s): Viallard,V.;Poirier,I.;Cournoyer,B.;Haurat,J.;Wiebkin,S.;Ophel-Keller,K.;Balandreau,J.
Journal: Int J Syst Bacteriol
Title: Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia
Volume: 48 Pt 2
Page(s): 549-63
Year: 1998
Keyword(s): GENBANK/U96927 GENBANK/U96928 GENBANK/U96929 GENBANK/U96930 GENBANK/U96931 GENBANK/U96932 GENBANK/U96933 GENBANK/U96934 GENBANK/U96935 GENBANK/U96936 GENBANK/U96937 GENBANK/U96938 GENBANK/U96939 GENBANK/U96940 GENBANK/U96941 Base Sequence Burkholderia/*classification/genetics/metabolism Carbohydrates/metabolism DNA, Bacterial Fatty Acids/analysis Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny Pseudomonas/*classification Support, Non-U.S. Gov't Triticum/microbiology Zea mays/microbiology
Remarks: In a survey of soil and wheat or maize rhizoplane bacteria isolated using a medium containing azelaic acid and tryptamine as sole carbon and nitrogen sources, respectively, a large proportion of Burkholderia-like bacteria were found. Among them, a homogeneous group of strains was identifiable based on phenotypic properties, fatty acid composition, DNA-DNA hybridizations and 16S rDNA sequences. According to molecular data, this group belongs to the genus Burkholderia but its weak similarity to previously described species suggests that it belongs to a novel species. Closest 16S rDNA phylogenetic neighbours of this species are Burkholderia caryophylli and two previously named Pseudomonas species which clearly appear to be part of the Burkholderia genus and were thus named Burkholderia glathei comb. nov. and Burkholderia phenazinium comb. nov. Strains of the new species are oxidase- and catalase-positive, produce indole and gelatinase, and use L-xylose, lactose, rhamnose, trehalose, D-lyxose, L-arabitol, xylitol and D-raffinose as sole carbon source. This novel taxon is named Burkholderia graminis. In the course of this study, [Pseudomonas] pyrrocinia also proved to be a member of the Burkholderia genus.
URL: 98401484
Ref #: 12332
Author(s): LiPuma,J.J.;Dulaney,B.J.;McMenamin,J.D.;Whitby,P.W.;Stull,T.L.;Coenye,T.;Vandamme,P.
Journal: J Clin Microbiol
Title: Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients
Volume: 37
Page(s): 3167-70
Year: 1999
Keyword(s): GENBANK/AF097530 GENBANK/AF097531 GENBANK/AF097532 GENBANK/AF097533 GENBANK/AF097534 Base Sequence Burkholderia cepacia/classification/*isolation & purification Cystic Fibrosis/*microbiology Human Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sensitivity and Specificity Support, Non-U.S. Gov't
Remarks: PCR assays targeting rRNA genes were developed to identify species (genomovars) within the Burkholderia cepacia complex. Each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. These isolates were from clinical and environmental sources and included 107 B. cepacia complex strains, 23 Burkholderia gladioli strains, 20 Ralstonia pickettii strains, 10 Pseudomonas aeruginosa strains, 8 Stenotrophomonas maltophilia strains, and 9 isolates belonging to nine other species. The sensitivity and specificity of the 16S rRNA-based assay for Burkholderia multivorans (genomovar II) were 100 and 99%, respectively; for Burkholderia vietnamiensis (genomovar V), sensitivity and specificity were 87 and 92%, respectively. An assay based on 16S and 23S rRNA gene analysis of B. cepacia ATCC 25416 (genomovar I) was useful in identifying genomovars I, III, and IV as a group (sensitivity, 100%, and specificity, 99%). Another assay, designed to be specific at the genus level, identified all but one of the Burkholderia and Ralstonia isolates tested (sensitivity, 99%, and specificity, 96%). The combined use of these assays offers a significant improvement over previously published PCR assays for B. cepacia.
URL: 99419133
Ref #: 1786
Author(s): Palleroni,N.J.;Holmes,B.
Journal: Int. J. Syst. Bacteriol.
Title: Pseudomonas cepacia sp. nov., nom. rev.
Volume: 31
Page(s): 479-481
Year: 1981
Ref #: 4854
Author(s): Palleroni,N.J.;Kunisawa,R.;Contopoulou,R.;Doudoroff,M.
Journal: Int. J. Syst. Bacteriol.
Title: Nucleic acid homologies in the genus Pseudomonas.
Volume: 23
Page(s): 333-339
Year: 1973
Ref #: 4855
Author(s): Meyer,J.-M.;Hohnadel,D.;Hallé,F.
Journal: J. Gen. Microbiol.
Title: Cepabactin from Pseudomonas cepacia, a new type of siderophore.
Volume: 135
Page(s): 1479-1487
Year: 1989
Ref #: 4899
Author(s): Dewhirst,F.E.;Paster,B.J.;Bright,P.L.
Journal: Int. J. Syst. Bacteriol.
Title: Chromobacterium, Eikenella, Kingella, Neisseria, Simonsiella, and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Nei
Volume: 39
Page(s): 258-266
Year: 1989
Ref #: 5966
Author(s): Gillis,M.;Van,T.V.;Bardin,R.;Goor,M.;Hebbar,P.;Willems,A.;Segers,P.;Kersters,K.;Heulin,T.;Fernandez,M.P.
Journal: Int. J. Syst. Bacteriol.
Title: Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam.
Volume: 45
Page(s): 274-289
Year: 1995
Data: (ATCC 25416) ATCC in 1971 / R. W. Ballard / M. P. Starr / W. H. Burkholder / Onion / Burkholder, W. H. (1950) Phytopathology, 40, 115 / Ballard, R. W. et al. (1970) J. gen. Microbiol. 60, 199
Accession Date: 01/01/1971
History: BALLARD W H ,STARR M P ,ISOLATED BY BURKHOLDER W H ,1950 PRE:FR
Authority: (Palleroni and Holmes 1981) Yabuuchi et al. 1993
Depositor: ATCC
Taxonomy: TaxLink: S2421 (Pseudomonas cepacia) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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